Article
Environmental Sciences
Zhongquan Jiang, Yutong Sun, Huize Guan, Danqing Sun, Shu Fang, Xuening Ma, Zhongyang Wang, Zhen Li, Chunhua Zhang, Ying Ge
Summary: The study found that when exposed to low concentrations of As(V), Chlamydomonas reinhardtii accumulated starch and produced more extracellular polysaccharides, while under high concentrations of As(V), the cells redirected carbon flux and transformed lipids into polysaccharides, indicating the critical involvement of polysaccharides in As(V) resistance in C. reinhardtii.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Aoxue Sun, Limei Xu, Gaoxiang Zhou, Erqin Yin, Tiantian Chen, Yong Wang, Xiaochen Li
Summary: This study investigated the impacts of micro-plastics as carriers on the toxicity of heavy metals. It found that nano-sized plastics showed higher adsorption capacities and lower desorption rates for Pb2+ compared to micro-sized plastics. The presence of micro-plastics decreased the bioavailability of Pb2+ to Chlamydomonas reinhardtii and suppressed membrane damage and oxidative stress. The study also observed that the size of micro-plastics influenced their capacity to carry heavy metal ions and their toxicity to C. reinhardtii.
Article
Multidisciplinary Sciences
Tomokazu Kurita, Masako Iwai, Keishi Moroi, Kumiko Okazaki, Seiji Nomura, Fumihiko Saito, Shinichiro Maeda, Akihide Takami, Atsushi Sakamoto, Hiroyuki Ohta, Tetsushi Sakuma, Takashi Yamamoto
Summary: Algal lipids, produced through photosynthesis and carbon dioxide assimilation, are expected to be the basis for sustainable fuels. However, the use of genetically modified organisms (GMOs) in outdoor culture is restricted. Recent research has discovered that plasmids containing yeast centromere and autonomous replication sequence can behave as episomes in Nannochloropsis species. This study aims to develop a genome editing system that allows the removal of expression vectors after introducing mutations, thereby creating mutated Nannochloropsis without any transgenes.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Environmental
Vera Slaveykova, Sanghamitra Majumdar, Nicole Regier, Weiwei Li, Arturo A. Keller
Summary: Metabolomics was used to study the response of green alga to sublethal concentrations of inorganic mercury and monomethylmercury. The alga adjusted its metabolism by increasing levels of various metabolites involved in different pathways after exposure to low concentrations of mercury compounds. Exposure to inorganic mercury resulted in more pronounced metabolic perturbations in comparison to monomethylmercury, while most metabolic disturbances were common between the two treatments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ashish K. Maurya, Piali Sengupta
Summary: The paper introduces a novel cilia regulator called XBX-4, which modulates the CCRK and MAK kinase pathway in C. elegans. Mutations associated with Joubert syndrome in the XBX-4 DUF3719 domain also result in cilia elongation.
Article
Plant Sciences
Shun Tamaki, Kazunari Ozasa, Toshihisa Nomura, Marumi Ishikawa, Koji Yamada, Kengo Suzuki, Keiichi Mochida
Summary: Zeaxanthin is required for the formation of functional photoreactive organelles in Euglena gracilis, highlighting the evolutionary differences between euglenophytes and chlorophytes in the photosensing mechanism. The study demonstrates the metabolic regulation of photoreactive organelle formation in Euglena gracilis and its importance in eyespot formation.
Article
Biotechnology & Applied Microbiology
Antonia Schad, Sonja Roessler, Raimund Nagel, Heiko Wagner, Christian Wilhelm
Summary: Research has shown that efficient excretion of glycolate can be achieved by mutating the carbon concentrating mechanisms and the C2 cycle in Chlamydomonas reinhardtii. This is significant for the production of chemical building blocks and the advancement of algal-based biotechnology.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Xiaosong Gu, Li Cao, Xiaoying Wu, Yanhua Li, Qiang Hu, Danxiang Han
Summary: This study identified galactosyl hydrolases (CrGH) in lipid bodies of Chlamydomonas reinhardtii and found that deficiency of CrGH led to an increase in MGDG and DGDG content and a decrease in TAG content under high light stress conditions. The up-regulation of CrGH under high light stress suggests its involvement in the conversion of photosynthetic membranes to TAGs, revealing a new paradigm for lipid metabolism in microalgae.
Article
Chemistry, Analytical
Maria Teresa Giardi, Daniele Zappi, Mehmet Turemis, Gabriele Varani, Fabrizio Lo Celso, Giampaolo Barone, Pietro Calandra, Amina Antonacci, Viviana Scognamiglio
Summary: This passage describes a method to design and synthesize novel artificial peptides that mimic the plastoquinone binding niche of the D1 protein from Chlamydomonas reinhardtii, with the ability to bind herbicides. These peptides were then functionalized with quantum dots for the development of a hybrid optosensor for the detection of atrazine, meeting E.U. legislation requirements with detection limits in the mu g/L concentration range.
Article
Plant Sciences
Yaqi An, Dong Wang, Jingxia Du, Xinwei Wang, Jianwei Xiao
Summary: The carbon dioxide emitted by human accounts for only a small fraction of global photosynthesis consumption, half of which is due to microalgae. The high efficiency of algae photosynthesis is attributed to the pyrenoid-based CO2-concentrating mechanism (CCM). The formation of pyrenoid which has a variety of Rubisco-binding proteins mainly depends on liquid-liquid phase separation (LLPS) of Rubisco, a CO2 fixing enzyme. The current research on the structure, assembly and application of Chlamydomonas reinhardtii pyrenoids is summarized in this article, providing new ideas for improving crop photosynthetic performance and yield.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Nan Lil, Sakuya Nakamura, Silvia Ramundo, Yoshiki Nishimura, Shinya Hagihara, Masanori Izumi
Summary: The study investigated the role of autophagy in chloroplast protein degradation in Chlamydomonas cells, revealing that chloroplast proteotoxic stress induced by inhibition of intraorganellar protease led to transport of chloroplast proteins to vacuoles, which was delayed by autophagy inhibitors. The findings suggest that autophagy contributes to chloroplast protein degradation in Chlamydomonas cells.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Julian Wichmann, Annibel Eggert, Liam D. H. Elbourne, Ian T. Paulsen, Kyle J. Lauersen, Olaf Kruse
Summary: In this study, the accessibility of different isoprenoid precursor pools for sesquiterpenoid production in Chlamydomonas reinhardtii was investigated. It was found that both the cytoplasm and chloroplast can be utilized for the generation of sesquiterpenoids. Targeting the fusion protein to the chloroplast and increasing gene dosage significantly improved sesquiterpene production. In addition, potential prenyl unit transporters were proposed based on bioinformatic analyses, which could enhance the capabilities of sesquiterpenoid production.
MICROBIAL CELL FACTORIES
(2022)
Article
Environmental Sciences
Yan Huang, Jinlong Lai, Yang Huang, Xuegang Luo, Xu Yang, Zewei Liu, Yue Duan, Chen Li
Summary: This study investigated the effects of nano-BiOI on freshwater microalgae and its toxicological mechanisms. The results showed that nano-BiOI stimulated algal growth at low concentrations but inhibited growth at higher concentrations. Nano-BiOI induced the generation of intracellular reactive oxygen species and interfered with the mineral nutrient metabolism of the algae. Metabolomics analysis revealed significant alterations in metabolic pathways under high concentrations of nano-BiOI.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Environmental Sciences
Fangbing Qi, Yu Gao, Jiaqi Liu, Xiangyu Yao, Kai Han, Ziyi Wu, Yingjuan Wang
Summary: Cadmium (Cd) pollution is a threatening challenge to the environment and human health, with serious impacts on natural organisms. Green algae, such as Chlamydomonas reinhardtii (C. reinhardtii), have the potential to be a safer and more cost-effective approach for treating heavy metal ions in wastewater due to their sorption properties. However, C. reinhardtii is affected by heavy metal ions when sorbed. Melatonin can protect plants from damage under stress conditions. In this study, the effects of melatonin on C. reinhardtii under Cd stress were investigated, including cell morphology, chlorophyll content, enzymatic activity, gene expression, and the AsA-GSH cycle. The presence of melatonin effectively protected the activity of photosynthetic system II, enhanced antioxidant activity, upregulated gene expression in the AsA-GSH cycle, and reduced the level of ROS, alleviating the damage caused by Cd toxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Zihui Huang, Liangliang Shen, Wenda Wang, Zhiyuan Mao, Xiaohan Yi, Tingyun Kuang, Jian-Ren Shen, Xing Zhang, Guangye Han
Summary: Photosystems I and II undergo state transitions to balance light energy distribution and optimize photosynthesis, with the cryo-EM structure of a PSI-LHCI-LHCII supercomplex from C. reinhardtii revealing subunit organization and energy transfer pathways.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Moyan Jia, Jacob Munz, Jenny Lee, Nolan Shelley, Yuan Xiong, Sunjoo Joo, EonSeon Jin, Jae-Hyeok Lee
Summary: Our study identified and characterized the bHLH factor NRI1 in Chlamydomonas, which is required to repress the use of alternative nitrogen sources. NRI1 functions as a critical repressor and coordinates diverse nitrogen starvation-induced responses, providing a crucial first step in understanding how plants and algae cope with fluctuating nitrogen availability.
Article
Multidisciplinary Sciences
Sunjoo Joo, Thamali Kariyawasam, Minjae Kim, EonSeon Jin, Ursula Goodenough, Jae-Hyeok Lee
Summary: OTU2p in Chlamydomonas is a protein that prevents the degradation of organelles in plus mating-type gametes, driving uniparental organelle inheritance without gametic dimorphism.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Leila R. Fletcher, Christine Scoffoni, Colin Farrell, Thomas N. Buckley, Matteo Pellegrini, Lawren Sack
Summary: Research has found that there is a decoupling between relative growth rate and adaptation to cold or dry climates in Arabidopsis ecotypes. This decoupling is consistent with the presence of multiple stress resistance and avoidance mechanisms, contributing to the wide geographic range and resilience of the species.
Article
Multidisciplinary Sciences
Julie Bethany, Shannon Lynn Johnson, Ferran Garcia-Pichel
Summary: This study describes a novel obligate, intracellular predatory bacterium that preys on soil cyanobacteria in biocrusts, impacting crucial biocrust properties and ecosystem productivity.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Dohun Kim, Jae-Hyeok Lee, Si-Wook Kim, Jong-Myeon Hong, Sung-Jin Kim, Minji Song, Jong-Mun Choi, Sun-Yeop Lee, Hongjun Yoon, Jin-Young Yoo
Summary: This study developed an AI model to estimate the pneumothorax amount on chest radiographs and analyzed the clinical outcomes. The results showed a high accuracy of the AI model, but with some differences compared to the true label.
Article
Microbiology
Fabio Palmieri, Pauline Udriet, Shannon L. Johnson, Karen Davenport, Patrick S. G. Chain, Saskia Bindschedler, Pilar Junier
Summary: In this study, we sequenced and analyzed the complete genome of the soil oxalotrophic bacterium Cupriavidus oxalaticus Ox1 and a genetically modified strain with mCherry tag. The genome is approximately 6.69 Mb in size, with a GC content of 66.9%. The genome of C. oxalaticus Ox1 contains a complete operon responsible for the degradation and assimilation of oxalate.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Genetics & Heredity
J. Mitchell McGrath, Andrew Funk, Paul Galewski, Shujun Ou, Belinda Townsend, Karen Davenport, Hajnalka Daligault, Shannon Johnson, Joyce Lee, Alex Hastie, Aude Darracq, Glenda Willems, Steve Barnes, Ivan Liachko, Shawn Sullivan, Sergey Koren, Adam Phillippy, Jie Wang, Tiffany Liu, Jane Pulman, Kevin Childs, Shengqiang Shu, Anastasia Yocum, Damian Fermin, Effie Mutasa-Gottgens, Piergiorgio Stevanato, Kazunori Taguchi, Rachel Naegele, Kevin M. Dorn
Summary: A contiguous assembly of the 'EL10' sugar beet genome was constructed using PacBio long-read sequencing, BioNano optical mapping, Hi-C scaffolding, and Illumina short-read error correction. The EL10.1 assembly is highly congruent with the published sugar beet reference genome, with 96.2% of the genome contained in nine chromosome-sized pseudomolecules.
Article
Biochemistry & Molecular Biology
Rory J. Craig, Sean D. Gallaher, Shengqiang Shu, Patrice A. Salome, Jerry W. Jenkins, Crysten E. Blaby-Haas, Samuel O. Purvine, Samuel O'Donnell, Kerrie Barry, Jane Grimwood, Daniela Strenkert, Janette Kropat, Chris Daum, Yuko Yoshinaga, David M. Goodstein, Olivier Vallon, Jeremy Schmutz, Sabeeha S. Merchant
Summary: The article presents a major update to the Chlamydomonas reference genome and structural annotations, revealing large genomic changes in laboratory culture. The authors improved assembly quality and annotations, generated chromosome-level assemblies for two laboratory strains, and validated their results through linkage analyses. They also updated gene symbols and textual annotation of functionally characterized genes, and discovered genomic instability in the classical reference strain. The resources presented in this article will pave the way for further research in Chlamydomonas genomics.
Article
Multidisciplinary Sciences
Sangwoo Moon, Jae Hyeok Lee, Hyunju Choi, Sun Yeop Lee, Jiwoong Lee
Summary: This study aimed to develop a deep learning model to predict 10-2 visual field using wide-field swept-source optical coherence tomography images. By using the Inception-ResNet-V2 model and combining macular ganglion cell/inner plexiform layer thickness maps with retinal nerve fiber layer thickness maps, the model effectively predicted 10-2 visual field.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Marco Morselli, Ronan Bennett, Nikko-Ideen Shaidani, Marko Horb, Leonid Peshkin, Matteo Pellegrini
Summary: This study finds that age-associated changes in DNA methylation also exist in amphibians, and whole-genome bisulfite sequencing (WGBS) profiles from skin samples of nine frogs were used to characterize the gene- and chromosome-scale features of age-associated DNA methylation changes. The results suggest that the mechanism of DNA methylation changes in amphibians is similar to that in mammals. Additionally, a targeted DNA methylation analysis (TBSseq) assay was developed for future studies with larger cohorts of individuals. The findings of this study will contribute to understanding the relationship between DNA methylation and other hallmarks of aging using the unique resources available for Xenopus.
Article
Biology
Chang Ho Kang, Jae Hyeok Lee, Yeon-Ju Kim, Cha Young Kim, Soo In Lee, Jong Chan Hong, Chae Oh Lim
Summary: This study investigates the biochemical and molecular functions of the Arabidopsis thaliana BAG2 (AtBAG2) protein and its physiological role under stress conditions. The results show that AtBAG2 has molecular chaperone activity attributed to its BAG domain and this activity is essential for the heat stress response in Arabidopsis.
Correction
Endocrinology & Metabolism
David A. Salisbury, David Casero, Zhengyi Zhang, Dan Wang, Jason Kim, Xiaohui Wu, Laurent Vergnes, Aashiq H. Mirza, Paola Leon-Mimila, Kevin J. Williams, Adriana Huertas-Vazquez, Samie R. Jaffrey, Karen Reue, Jianjun Chen, Tamer Sallam
Article
Cell Biology
Giulia Protti, Liudmilla Rubbi, Tarik Goren, Ramazan Sabirli, Serkan Civlan, Ozgur Kurt, Ibrahim Turkcuer, Aylin Koseler, Matteo Pellegrini
Summary: This study aimed to investigate the association between the methylome of buccal epithelial cells and demographic and health-related variables. Multiple factors, including age, sex, blood immune composition, and hemoglobin levels, were found to accurately model DNA methylation variation. The identified genomic loci associated with these factors were enriched in relevant transcription factors, drugs, and diseases. This study contributes to a better understanding of human methylome plasticity and highlights the potential clinical implications of considering these physiological traits in medical epigenomic investigations.
PHYSIOLOGICAL GENOMICS
(2023)
Article
Multidisciplinary Sciences
Lital Davidi, Sean D. Gallaher, Eyal Ben-David, Samuel O. Purvine, Thomas L. Fillmore, Carrie D. Nicora, Rory J. Craig, Stefan Schmollinger, Sanja Roje, Crysten E. Blaby-Haas, Robert P. Auber, Jennifer H. Wisecaver, Sabeeha S. Merchant
Summary: This article investigates the adaptation mechanisms of two marine algae species under iron-deficient conditions. The study reveals multiple iron uptake routes in these species and their ability to adjust protein expression and structure to cope with iron deficiency.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Correction
Endocrinology & Metabolism
David A. Salisbury, David Casero, Zhengyi Zhang, Dan Wang, Jason Kim, Xiaohui Wu, Laurent Vergnes, Aashiq H. Mirza, Paola Leon-Mimila, Kevin J. Williams, Adriana Huertas-Vazquez, Samie R. Jaffrey, Karen Reue, Jianjun Chen, Tamer Sallam