期刊
EUKARYOTIC CELL
卷 13, 期 9, 页码 1143-1157出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.00092-14
关键词
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资金
- Slovak grant agency APVV [0035-11, 0123-10]
- Slovak grant agency VEGA [1/0311/12, 1/0405/11]
- National Institutes of Health [2R01ES013773-06A1]
- Japan Society for the Promotion of Science [22570213]
- Core Research for Evolutional Science and Technology (CREST)
- Grants-in-Aid for Scientific Research [22570213] Funding Source: KAKEN
Mitochondrial DNA (mtDNA) is highly compacted into DNA-protein structures termed mitochondrial nucleoids (mt-nucleoids). The key mt-nucleoid components responsible for mtDNA condensation are HMG box-containing proteins such as mammalian mitochondrial transcription factor A (TFAM) and Abf2p of the yeast Saccharomyces cerevisiae. To gain insight into the function and organization of mt-nucleoids in strictly aerobic organisms, we initiated studies of these DNA-protein structures in Yarrowia lipolytica. We identified a principal component of mt-nucleoids in this yeast and termed it YlMhb1p (Y.lipolytica mitochondrial HMG box-containing protein 1). YlMhb1p contains two putative HMG boxes contributing both to DNA binding and to its ability to compact mtDNA in vitro. Phenotypic analysis of a Delta mhb1 strain lacking YlMhb1p resulted in three interesting findings. First, although the mutant exhibits clear differences in mt-nucleoids accompanied by a large decrease in the mtDNA copy number and the number of mtDNA-derived transcripts, its respiratory characteristics and growth under most of the conditions tested are indistinguishable from those of the wild-type strain. Second, our results indicate that a potential imbalance between subunits of the respiratory chain encoded separately by nuclear DNA and mtDNA is prevented at a (post) translational level. Third, we found that mtDNA in the Delta mhb1 strain is more prone to mutations, indicating that mtHMG box-containing proteins protect the mitochondrial genome against mutagenic events.
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