Article
Microbiology
Yisheng Chen, Yongqin Wu, Kaiyi Lulou, Dongting Yao, Chunmei Ying
Summary: This study investigated the antifungal susceptibility and molecular epidemiology of vaginal and non-vaginal Candida glabrata isolates. The results showed high resistance rates to fluconazole, itraconazole, and voriconazole, while all isolates remained susceptible to amphotericin B. Genotyping revealed partially shared genotypes between vaginal and non-vaginal samples, with additional genotypes found in vaginal samples. The majority of resistant strains correlated with the predominant genotype.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Eun Jeong Won, Min Ji Choi, Seok Hoon Jeong, Dokyun Kim, Kyeong Seob Shin, Jeong Hwan Shin, Young Ree Kim, Hyun Soo Kim, Young Ah Kim, Young Uh, Namhee Ryoo, Jeong Su Park, Kyoung Un Park, Seung A. Byun, Ga Yeong Lee, Soo Hyun Kim, Jong Hee Shin
Summary: This study incorporated nationwide Candida antifungal surveillance into the Korea Global Antimicrobial Resistance Surveillance System. The results showed a high proportion of Candida species among blood pathogens, with a majority originating in hospitals and occurring in intensive care unit patients. Furthermore, non-susceptibility and resistance to commonly used antifungal drugs were identified in certain Candida isolates.
Article
Microbiology
Pao-Yu Chen, Yu-Chung Chuang, Un-In Wu, Hsin-Yun Sun, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, Shan-Chwen Chang
Summary: The study found that azole resistance and trailing in Candida tropicalis differ phenotypically and genotypically, mainly due to mutations and up-regulation of ERG11 and MDR1 genes.
Article
Microbiology
Erick Martinez-Herrera, Maria Guadalupe Frias-De-Leon, Rigoberto Hernandez-Castro, Eduardo Garcia-Salazar, Roberto Arenas, Esther Ocharan-Hernandez, Carmen Rodriguez-Cerdeira
Summary: There is variability in the rates of resistance to antifungal agents in Candida glabrata in different regions worldwide. Limited data are available on the antifungal resistance in C. glabrata in Ibero-America. A systematic review was conducted to determine the antifungal resistance in C. glabrata in this region over the last five years. The use of azoles and echinocandins varied among different countries. The limited information emphasizes the need for species identification and antifungal susceptibility tests to prevent the development of antifungal resistance.
Article
Microbiology
Irene Stefanini, Emily Stoakes, Houdini H. T. Wu, Li Xu-McCrae, Abid Hussain, John Moat, Christopher G. Dowson, Miruna D. David, Chrystala Constantinidou
Summary: By analyzing a large set of strains using multiple methods, this study revealed strict relationships between large chromosomal rearrangements in C. glabrata and phylogenetic clades, genes subjected to different selective pressures, and new sets of genes associated with resistance to antifungals. These findings contribute to our understanding of C. glabrata evolution and epidemiology and provide a foundation for the future development of tailored therapeutic approaches.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Judith Diaz-Garcia, Aina Mesquida, Ana Gomez, Marina Machado, Pablo Martin-Rabadan, Luis Alcala, Carlos Sanchez-Carrillo, Elena Reigadas, Teresa Vicente, Patricia Munoz, Pilar Escribano, Jesus Guinea
Summary: The study identified compartmentalization of antifungal resistance in Candida isolates, with higher resistance rates observed in abdominal cavity samples, especially in Candida glabrata. Resistance was mainly due to antifungal treatment rather than the spread of resistant clones.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Qiqi Wang, Yun Li, Xuan Cai, Ruoyu Li, Bo Zheng, Ence Yang, Tianyu Liang, Xinyu Yang, Zhe Wan, Wei Liu
Summary: In this study, multidrug-resistant isolates of Candida glabrata exhibited resistance through different mechanisms. Overexpression of CDR1 and ERG11 in one isolate, and only overexpression of CDR1 in the other, may mediate resistance to POS. The S663P mutation in FKS2 and up-regulation of FKS2 in both isolates may contribute to echinocandin resistance.
Article
Infectious Diseases
Kento Furuya, Kenta Ito, Kyohei Sugiyama, Satoshi Tokuda, Hideyuki Kanemoto, Katsuhiko Kamei, Toshio Shimada
Summary: This is a case report of a 73-year-old male patient who developed a pancreaticoduodenal fistula infection after pancreaticoduodenectomy and had co-infectious bloodstream infection with S. cerevisiae and Candida glabrata. It is rare to detect both fungi in blood cultures together. Micafungin was initially used for treatment, but S. cerevisiae was detected during its administration, suggesting that micafungin may not be effective against this strain.
BMC INFECTIOUS DISEASES
(2023)
Article
Microbiology
Tsidiso G. Maphanga, Serisha D. Naicker, Stanford Kwenda, Jose F. Munoz, Erika van Schalkwyk, Jeannette Wadula, Trusha Nana, Arshad Ismail, Jennifer Coetzee, Chetna Govind, Phillip S. Mtshali, Ruth S. Mpembe, Nelesh P. Govender
Summary: A study on Candida auris isolates from South African hospitals revealed a high prevalence of multidrug resistance, particularly to azoles. Even phenotypically susceptible isolates showed mutations in resistance genes, indicating a complex pattern of antifungal resistance in this fungal pathogen.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Lance R. Fredericks, Mark D. Lee, Hannah R. Eckert, Shunji Li, Mason A. Shipley, Cooper R. Roslund, Dina A. Boikov, Emily A. Kizer, Jack D. Sobel, Paul A. Rowley
Summary: Candida glabrata is more susceptible to inhibition by Saccharomyces killer yeasts compared to other species of Candida yeasts, despite showing high resistance to antifungal therapeutics.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Mycology
A. Arastehfar, M. Marcet-Houben, F. Daneshnia, S. J. Taj-Aldeen, D. Batra, S. R. Lockhart, E. Shor, T. Gabaldon, D. S. Perlin
Summary: This study provides long-read based assemblies to simplify the multilocus sequence typing (MLST) scheme for C. glabrata. A comparative genomic analysis identified highly polymorphic loci (HPL) which proved to be highly resolutive for sequence typing (ST). A new typing approach combining single nucleotide polymorphisms (SNPs) and insertions and deletions (indels) at two loci significantly simplified identification of ST in C. glabrata.
STUDIES IN MYCOLOGY
(2021)
Article
Microbiology
Qiushi Zheng, Jing Liu, Juanxiu Qin, Bingjie Wang, Jian Bing, Han Du, Min Li, Fangyou Yu, Guanghua Huang
Summary: This study reports the population structure and ploidy states of 500 clinical isolates of Candida glabrata from China. Surprisingly, the ploidy of a subset of clinical isolates varied dramatically, with some able to switch to aneuploid, diploid, or hyperdiploid forms. Cells with different ploidy in C. glabrata exhibited differences in morphology, antifungal susceptibility, virulence, and global gene expression profile. Ploidy switching could be a strategy for rapid adaptation to environmental changes and an alternative to sexual reproduction in this fungus.
Article
Mycology
M. Doman, L. Makrai, Gy. Lengyel, R. Kovacs, L. Majoros, K. Banyai
Summary: The genetic diversity and population structure of avian origin Candida albicans isolates were characterized using multilocus sequence typing in this study. The results showed a fairly diverse genetic construction of the avian origin C. albicans population, with some degree of separation observed between bird and human strains in evolution.
Article
Infectious Diseases
Serisha D. Naicker, Liliwe Shuping, Thokozile G. Zulu, Ruth S. Mpembe, Mabatho Mhlanga, Ernest M. Tsotetsi, Tsidiso G. Maphanga, Nelesh P. Govender, GERMS SA
Summary: Nakaseomyces glabrata is a common cause of candidaemia in South Africa, particularly in older female patients admitted to public hospitals and post-surgery patients. Many of these patients are treated with suboptimal doses of fluconazole, and a small percentage of N. glabrata isolates show resistance to echinocandins and fluconazole. Echinocandins or polyenes are recommended for the treatment of confirmed N. glabrata candidaemia.
Article
Biochemistry & Molecular Biology
Noura Nouri, Shahla Roudbar Mohammadi, Justin Beardsley, Peyman Aslani, Fatemeh Ghaffarifar, Maryam Roudbary, Celia Fortuna Rodrigues
Summary: The number of Candida spp. infections and drug resistance are increasing globally, and new antifungal compounds are urgently needed. Thymoquinone (TQ), a bioactive compound in black cumin seed, was found to have antifungal and antibiofilm activity against Candida glabrata, a high-priority pathogen. TQ inhibited biofilm formation and reduced EPA6 gene expression in C. glabrata isolates.