Article
Engineering, Environmental
Vera Slaveykova, Sanghamitra Majumdar, Nicole Regier, Weiwei Li, Arturo A. Keller
Summary: Metabolomics was used to study the response of green alga to sublethal concentrations of inorganic mercury and monomethylmercury. The alga adjusted its metabolism by increasing levels of various metabolites involved in different pathways after exposure to low concentrations of mercury compounds. Exposure to inorganic mercury resulted in more pronounced metabolic perturbations in comparison to monomethylmercury, while most metabolic disturbances were common between the two treatments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Microbiology
Emma O. Fuentes-Ramirez, Miriam Vazquez-Acevedo, Alfredo Cabrera-Orefice, Sergio Guerrero-Castillo, Diego Gonzalez-Halphen
Summary: This study provides a morphological and proteomic characterization of the colorless alga Polytomella parva, identifying several plastid proteins involved in various metabolic pathways. The results contribute to a better understanding of the metabolism and biological activities of this unicellular, free-living alga.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Zihui Huang, Liangliang Shen, Wenda Wang, Zhiyuan Mao, Xiaohan Yi, Tingyun Kuang, Jian-Ren Shen, Xing Zhang, Guangye Han
Summary: Photosystems I and II undergo state transitions to balance light energy distribution and optimize photosynthesis, with the cryo-EM structure of a PSI-LHCI-LHCII supercomplex from C. reinhardtii revealing subunit organization and energy transfer pathways.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Ulrich Kueck, Olga Schmitt
Summary: This article reviews RNA trans-splicing process in eukaryotes, focusing on trans-splicing of discontinuous group II introns in lower eukaryotes and plants' chloroplasts and mitochondria. It also discusses the origin of intronic sequences and the evolutionary relationship between chloroplast ribonucleoprotein complexes and the nuclear spliceosome.
Article
Biotechnology & Applied Microbiology
Fan Bai, Yu Zhang, Jin Liu
Summary: bZIP transcription factors play important roles in lipid and pigment metabolisms in microalgae, as shown by the comparative analysis of crbzip2 mutant and wild-type strain in Chlamydomonas reinhardtii. The disruption of CrBZIP2 leads to reduced TAG accumulation, increased DGDG production, and up-regulated biosynthetic pathways of carotenoids and chlorophylls. These findings suggest that CrBZIP2 could be a potential target for genetic engineering to manipulate lipid and pigment production in microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Plant Sciences
Xiaosong Gu, Li Cao, Xiaoying Wu, Yanhua Li, Qiang Hu, Danxiang Han
Summary: This study identified galactosyl hydrolases (CrGH) in lipid bodies of Chlamydomonas reinhardtii and found that deficiency of CrGH led to an increase in MGDG and DGDG content and a decrease in TAG content under high light stress conditions. The up-regulation of CrGH under high light stress suggests its involvement in the conversion of photosynthetic membranes to TAGs, revealing a new paradigm for lipid metabolism in microalgae.
Article
Microbiology
Akihito Nakanishi, Nanami Ozawa, Masahiko Watanabe
Summary: UV-C irradiation can efficiently collect intracellular lipids from green algae cells without cell burst and microbial contamination, while also potentially affecting lipid synthesis and degradation pathways. This study provides the first report on the transcriptional response of C. reinhardtii to UV-C irradiation.
Article
Multidisciplinary Sciences
Vivien Hotter, David Zopf, Hak Joong Kim, Anja Silge, Michael Schmitt, Prasad Aiyar, Johanna Fleck, Christian Matthaus, Julian Hniopek, Qing Yan, Joyce Loper, Severin Sasso, Christian Hertweck, Jurgen Popp, Maria Mittag
Summary: The interaction between algae and bacteria involves a complex molecular strategy, with bacteria using protegencin and orfamide A to disrupt the visual system and behavior of algae. This study highlights the multifaceted ways in which microorganisms can impact the growth and development of algae in nature.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Robert A. Freudenberg, Luisa Wittemeier, Alexander Einhaus, Thomas Baier, Olaf Kruse
Summary: This study systematically investigated the native putrescine metabolism in the green microalga Chlamydomonas reinhardtii and successfully achieved CO2-based bio-production of putrescine. The study provides important insights into the putrescine biosynthesis pathway and offers a promising approach for sustainable biotechnology.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Engineering, Environmental
Ivan Liakh, Darya Harshkova, Pavel Hrouzek, Katerina Bisova, Anna Aksmann, Bartosz Wielgomas
Summary: The use of unicellular algae for removing xenobiotics from wastewaters is a rapidly developing field in environmental protection. Efficient phycoremediation requires biosorption, bioaccumulation, and biotransformation. However, the potential toxicity of biotransformation products and the resistance of analyzed algae strains to the mixture of compounds need to be considered. In this study, Chlamydomonas reinhardtii showed promise in removing diclofenac, a non-steroidal anti-inflammatory drug, through biosorption, bioaccumulation, and biotransformation processes.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Cell Biology
Darya Harshkova, Ivan Liakh, Vitali Bialevich, Kamila Ondrejmiskova, Anna Aksmann, Katerina Bisova
Summary: The study aimed to verify the hypothesis that diclofenac affects cell cycle progression in green algae. The research demonstrated that diclofenac delays cell division in the algae, resulting in a decrease in the number of daughter cells produced.
Article
Environmental Sciences
Yiling Li, Shanshan Shang, Jin Shang, Wen-Xiong Wang
Summary: The study systematically assessed the toxicity of multiple representative MOFs on a freshwater green alga model, finding that all tested MOFs significantly reduced chlorophyll content and inhibited algal growth, with NiCo-PYZ and Al-PMOF (Cu) being the most toxic materials. Different MOFs exhibited distinct toxic mechanisms, highlighting the need for attention to the ecotoxicological risks of MOFs.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biotechnology & Applied Microbiology
E. Hounslow, C. A. Evans, J. Pandhal, T. Sydney, N. Couto, T. K. Pham, D. James Gilmour, P. C. Wright
Summary: Physiological and proteomic changes between two green alga strains, C. reinhardtii and C. nivalis, under salinity stress were characterized. Results showed that C. nivalis accumulated more carbohydrates and fatty acids under salt stress compared to C. reinhardtii. The differences in protein abundance provided insights into the mechanism by which salt stress promotes fatty acid accumulation in C. nivalis.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Microbiology
Zhao Peng, Gai Liu, Kaiyao Huang
Summary: Research shows that compared to a model organism from the same genus, snow algae exhibit higher growth rates and photosynthetic efficiency in cold environments, indicating better cold tolerance. Snow algae adapt to rapid temperature decrease by efficiently regulating transcription of specific genes to optimize resource assimilation and metabolic pathways. Additionally, horizontally transferred genes critical for cold adaptation in other psychrophiles are also identified in snow algae.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Alexander Einhaus, Jasmin Steube, Robert Ansgar Freudenberg, Jonas Barczyk, Thomas Baier, Olaf Kruse
Summary: In this study, innovative synthetic biology and efficient metabolic engineering strategies were combined to redirect metabolic flux through the MEP pathway in the green microalgae, C. reinhardtii, for efficient heterologous diterpenoid synthesis. Engineering of key enzymes and overexpression of fusion proteins successfully increased the production of high-value diterpenoids. Through fully photoautotrophic high cell density cultivations, significant amounts of sclareol, a high-value diterpenoid, were produced, demonstrating the potential of green microalgae as a powerful phototrophic cell factory for industrial biotechnology.
METABOLIC ENGINEERING
(2022)
