Article
Microbiology
Joseph T. Smith Jr, Brianna Tylec, Arunasalam Naguleswaran, Isabel Roditi, Laurie K. Read
Summary: This study reveals the importance of mitochondrial mRNA editing in the developmental regulation of Trypanosoma brucei. The researchers found that temperature reduction and depletion of the differentiation-repressive kinase RDK1 can affect the metabolism of Trypanosoma brucei by altering the editing of mitochondrial cytochrome mRNAs.
Review
Genetics & Heredity
Pedro Morais, Hironori Adachi, Yi-Tao Yu
Summary: snRNAs are crucial components of the spliceosome, forming snRNPs with proteins to participate in splicing by base-pairing interactions with pre-mRNA. They undergo various RNA modifications, contributing to their unique properties and biological relevance in the splicing process.
FRONTIERS IN GENETICS
(2021)
Article
Immunology
Manon Geerts, Nick Van Reet, Sander Leyten, Raf Berghmans, Kat S. Rock, Theresa H. T. Coetzer, Lauren E-A Eyssen, Philippe Buscher
Summary: The newly developed T. b. gambiense inhibition ELISA (g-iELISA) based on the principle of antibody binding showed high specificity and sensitivity, making it suitable for regional laboratories in gHAT endemic countries for monitoring and surveillance.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemical Research Methods
Shasha Shi, Xueni Li, Rui Zhao
Summary: Researchers have identified a potential exon-definition complex and explored its role in the generation of circRNAs by examining the structure of the yeast spliceosomal E complex and detecting back-splicing products and intermediates. This study provides valuable insights into the mechanism of circRNA biogenesis in eukaryotic species.
Review
Plant Sciences
Maria Camila Rodriguez Gallo, R. Glen Uhrig
Summary: This mini-review summarizes the current knowledge of plant-specific protein kinase families involved in alternative splicing (AS) regulation and compares them to their counterparts in animals. The extensive phosphorylation of splicing-related proteins in plants is highlighted, underscoring the importance of investigating the impact of AS on protein and post-translational modifications.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sinclair Cooper, Elizabeth S. Wadsworth, Achim Schnaufer, Nicholas J. Savill
Summary: The mitochondrial DNA of protists of order Kinetoplastida consists of thousands of interlinked circular molecules. It includes two types of molecules, minicircles and maxicircles. Minicircles encode guide RNA (gRNA) genes that mediate post-transcriptional editing of maxicircle encoded genes. The human sleeping sickness parasite Trypanosoma brucei has a diverse set of minicircle classes, some of which contain decaying remnants of once functional gRNA genes. The sequencing and annotation of minicircle classes in T. brucei subspecies and isolates have provided valuable data for understanding their structure and transcription.
Article
Oncology
Yifan Li, Qiaozhen Zhu, Shuchang Zhou, Jiangtao Chen, Aoyu Du, Changjiang Qin
Summary: In this study, we identified a prognostic and immune-related marker for hepatocellular carcinoma (HCC) from the RNA splicing pathway. This marker, thioredoxin like 4A (TXNL4A), was found to be highly expressed in HCC tumors and correlated with HCC survival. Additionally, TXNL4A was found to be associated with CD8 T cell infiltration in HCC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Microbiology
Gustavo Bravo Ruiz, Michele Tinti, Melanie Ridgway, David Horn
Summary: VSG expression plays a crucial role in parasite virulence and is a fascinating subject in extreme biology. This study identified three candidate VSG regulators and demonstrated the role of CFB2 in controlling VSG expression through the VSG 3' UTR. Additionally, insights into the connections between VSG expression control, ribosomal protein expression, and cytokinesis were revealed.
Review
Cell Biology
James Budzak, Gloria Rudenko
Summary: The African trypanosome Trypanosoma brucei has a dense layer of Variant Surface Glycoprotein (VSG) coat that protects it from immune attack. High levels of RNA transcription and pre-mRNA processing, as well as chromatin rearrangement, are important for the abundant expression of VSG.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katherine Sortino, Brianna L. Tylec, Runpu Chen, Yijun Sun, Laurie K. Read
Summary: Uridine insertion/deletion RNA editing is a common post-transcriptional modification in kinetoplastid organisms. The RESC complex plays an important role in this process by modulating protein and RNA interactions. In this study, the roles of RESC13 and RESC14 are further explored using high-throughput sequencing on multiple transcripts. The findings suggest that RESC13 and RESC14 have both conserved and transcript-specific effects in editing.
Article
Biochemistry & Molecular Biology
Roberto Docampo, Anibal Eugenio Vercesi
Summary: This review discusses the mechanisms of mitochondrial oxidant generation and removal, and the involvement of Ca2+ in trypanosome cell death, highlighting the need for further studies on ROS generation, defense mechanisms, and mitochondrial permeability transition pore in trypanosomatids.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Multidisciplinary Sciences
Justin Y. Toh, Agathe Nkouawa, Saul Rojas Sanchez, Huafang Shi, Nikolay G. Kolev, Christian Tschudi
Summary: By targeting 86 transcripts through RNAi, 22 genes that positively or negatively regulate the progression towards infectivity in Trypanosoma brucei have been identified, including the previously unknown nucleic acid binding proteins CSD1 and CSD2. The study suggests a considerable overlap between the genes that regulate stumpy bloodstream form Trypanosoma and those that govern the development of metacyclic form parasites.
SCIENTIFIC REPORTS
(2021)
Article
Clinical Neurology
Ainara Elorza, Yamile Marquez, Jorge R. Cabrera, Jose Luis Sanchez-Trincado, Maria Santos-Galindo, Ivo H. Hernandez, Sara Pico, Juan Diaz-Hernandez, Ramon Garcia-Escudero, Manuel Irimia, Jose J. Lucas
Summary: Correction of mis-splicing events is a promising therapeutic approach for neurological diseases caused by splicing-affecting mutations. Next-generation RNA sequencing has enabled the investigation of global mis-splicing in diseased tissue to identify key pathogenic mis-spliced genes. Our new intersect-RNA-seq approach captures pathogenic global mis-splicing in the Huntington's disease striatum.
Article
Plant Sciences
Jingjing Zhang, Feifei Guo, Rui Zhou, Changpei Xiang, Yi Zhang, Jinhuan Gao, Guangzhao Cao, Hongjun Yang
Summary: This study utilized a combination of transcriptomics, proteomics, and network pharmacology analysis to identify the key transcription factors involved in the protective effects of Panax notoginseng saponins (PNS) against ischemia/reperfusion injury (IRI). The results indicated that PNS regulated nerve system development and inflammation while targeting critical TFs like ERCC2, NR4A3, and others. Additionally, network pharmacology analysis predicted the impact of PNS chemical components on these identified TFs.
Article
Clinical Neurology
Mahmoud-Reza Rafiee, Sara Rohban, Karen Davey, Jernej Ule, Nicholas M. Luscombe
Summary: Valosin-containing protein (VCP) is a hexameric ATPase associated with cellular activities and its mutations are linked to neurodegenerative diseases. The study reveals that VCP mutations impair the binding of transcriptional machinery and mRNA splicing proteins to RNA polymerase II (RNAPII), leading to aberrant alternative splicing and proteinopathy in neurodegeneration.
Article
Biochemistry & Molecular Biology
Ankita Srivastava, Nitika Badjatia, Ju Huck Lee, Bing Hao, Arthur Gunzl
NUCLEIC ACIDS RESEARCH
(2018)
Article
Plant Sciences
Mai Thanh Thi Nguyen, Phu Hoang Dang, Tu Ngoc Nguyen, Linh Thuy Thi Bui, Hai Xuan Nguyen, Tho Huu Le, Truong Nhat Van Do, Nhan Trung Nguyen
PHYTOCHEMISTRY LETTERS
(2018)
Article
Biochemistry & Molecular Biology
Ujwala Gosavi, Ankita Srivastava, Nitika Badjatia, Arthur Gunzl
MOLECULAR MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
K. Shanmugha Rajan, Yinzhou Zhu, Katerina Adler, Tirza Doniger, Smadar Cohen-Chalamish, Ankita Srivastava, Moran Shalev-Benami, Donna Matzov, Ron Unger, Christian Tschudi, Arthur Gunzl, Gordon G. Carmichael, Shulamit Michaeli
Article
Nursing
Fayette Nguyen Truax, Julie Low, Tessa Mochizuki, Setie Asfaha, Tu Ngoc Nguyen, Michael Carson, Shereen Katrak, Neha Shah, Duc Nguyen
Summary: The study aimed to increase LTBI screening, testing, and treatment among non-US-born Asian populations in community clinics. By implementing standardized risk assessment, provider training, and the use of a care coordinator, the number of LTBI patients started on treatment significantly increased within 4 months postintervention.
JOURNAL OF NURSING CARE QUALITY
(2022)
Article
Computer Science, Cybernetics
Tu Ngoc Nguyen, Chao Hong Shen, Phong Ba Le
Summary: This study explores the influence of transformational leadership on radical and incremental innovation, finding that TL has a greater effect on radical innovation. The mediating role of KMC between TL and innovation capability is supported, with the influence of KMC on specific aspects of innovation capability depending on the level of collaborative culture in an organization.
Article
Biochemistry & Molecular Biology
Ankita Srivastava, Daniela L. Ambrosio, Monika Tasak, Ujwala Gosavi, Arthur Gunzl
Summary: This passage discusses how the pre-mRNA splicing factor PRP19 is recruited into the spliceosome in humans and yeast, as well as the role of spliced leader trans splicing in Trypanosomatidae. It also highlights the discovery of essential proteins like PRC5 in cell viability and pre-mRNA splicing, leading to the formation of the PRP19-related complex (PRC) in trypanosomes, which interacts with unique proteins like SYF1, SYF3, and ISY1. These findings reveal a distinct pre-organization of proteins in trypanosomes compared to other organisms.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Geriatrics & Gerontology
Xiao Liu, Mai Khanh Le, Amber Yew Chen Lim, Emily Jiali Koh, Tu Ngoc Nguyen, Naveed Anjum Malik, Christopher Tsung Chien Lien, Jer En Lee, Lydia Shu Yi Au, James Alvin Yiew Hock Low, Shiou Liang Wee
Summary: This study aimed to explore the understanding and practice of frailty screening among different acute care professionals in Singapore and identify barriers and facilitators related to frailty screening and its implementation. The results showed that frailty was mainly understood as a physical and age-related concept but not fully comprehended. Screening for frailty in acute care was considered important for identifying high-risk patients, implementing targeted treatment and care, and supporting decision making and prognosis estimation. However, specific issues such as cooperation from patients/caregivers, acceptance from healthcare workers/hospital managers, the need for dedicated resources, guidelines for follow-up management, and consensus on measurement scope were identified as barriers and challenges.
Article
Veterinary Sciences
Megin C. Nichols, Paul Gacek, Quyen Phan, Kelly J. Gambino-Shirley, Lauren M. Gollarza, Morgan N. Schroeder, Alexandra Mercante, Jocelyn Mullins, Anna Blackstock, Mark E. Laughlin, Samantha M. Olson, Eugene Pizzo, Tu Ngoc Nguyen, Laurn Mank, Kimberly Holmes-Talbot, Alycia McNutt, Diane Noel, Anthony Muyombwe, Jafar H. Razeq, Mary Jane Lis, Bruce Sherman, Wayne Kasacek, Laura Whitlock, Nancy Strockbine, Haley Martin, Eshaw Vidyaprakash, Patrick McCormack, Matthew Cartter
Summary: The study aimed to identify sources of STEC O157 infection at Farm X and recommend public health measures. A case-control study found that patients were more likely to have sat on hay bales at the doe barn, and environmental samples from Farm X showed extensive contamination with STEC O157. Handwashing stations with soap, running water, and disposable towels should be provided at farms, and access to animal areas should be limited for children at risk. National recommendations are needed to reduce disease transmission.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Critical Care Medicine
Tung Phi Nguyen, Xuan Thi Phan, Tuan Huu Nguyen, Dai Quang Huynh, Linh Thanh Tran, Huy Minh Pham, Tu Ngoc Nguyen, Hieu Trung Kieu, Thao Thi Ngoc Pham
Summary: This study investigates the incidence of major bleeding in peripheral-ECMO patients and finds a correlation between major bleeding and higher in-hospital mortality. APTT > 72 seconds and fibrinogen < 2 g/L are identified as the strongest predicting factors for major bleeding events.
CRITICAL CARE RESEARCH AND PRACTICE
(2022)
Editorial Material
Multidisciplinary Sciences
Samantha L. Anderson, Peter G. Boyd, Andrzej Gladysiak, Tu N. Nguyen, Robert G. Palgrave, Dominik Kubicki, Lyndon Emsley, Darren Bradshaw, Matthew J. Rosseinsky, Berend Smit, Kyriakos C. Stylianou
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Zena Lapp, Rany Octaria, Sean M. M. O'Malley, Tu Ngoc Nguyen, Hannah Wolford, Ryan Crawford, Christina Moore, Paula Snippes Vagnone, Diane Noel, Nadezhda Duffy, Ali Pirani, Linda S. S. Thomas, Brittany Pattee, Claire Pearson, Sandra N. N. Bulens, Sophie Hoffman, Marion Kainer, Melissa Anacker, James Meek, Isaac See, Kyle J. J. Gontjes, Allison Chan, Ruth Lynfield, Meghan Maloney, Mary K. K. Hayden, Evan Snitkin, Rachel B. B. Slayton
Summary: Regional genomic surveillance can provide valuable insights into the transmission of carbapenem-resistant Enterobacterales (CRE) and identify the specific factors driving their spread in different regions. This study collected CRE isolates from healthcare facilities in three U.S. states to evaluate the potential of genomic surveillance in tracking bla(KPC)-carrying CRE (KPC-CRE). Whole-genome sequencing of 255 isolates from 214 patients revealed diverse transmission patterns and underlying drivers of KPC-CRE burden across the regions.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Geriatrics & Gerontology
Tam N. Nguyen, Tu N. Nguyen, Anh T. Nguyen, Hoa L. Nguyen, Robert J. Goldberg, Huong T. T. Nguyen, Thu T. H. Nguyen, Thanh X. Nguyen, Huyen T. T. Vu
Summary: The objective of this observational study was to examine the association between appendicular lean mass and frailty in adults aged 60 years and older. The study found that frail patients had significantly lower appendicular lean mass compared with non-frail outpatients, and higher appendicular lean mass was associated with significantly reduced odds for frailty. Therefore, assessing appendicular lean mass in older patients attending outpatient geriatric clinics is important.
JOURNAL OF FRAILTY & AGING
(2022)
Proceedings Paper
Geosciences, Multidisciplinary
Wei He, Fei Jiang, Weimin Ju, Tu Ngoc Nguyen, Meihong Fang, Qiaoning He, Chunhua Zhang
2019 IEEE INTERNATIONAL GEOSCIENCE AND REMOTE SENSING SYMPOSIUM (IGARSS 2019)
(2019)
Proceedings Paper
Engineering, Electrical & Electronic
Amr El-Keyi, Hamza Umit Sokun, Tu Ngoc Nguyen, Qiubo Ye, Haiying Julie Zhu, Halim Yanikomeroglu
2017 IEEE 28TH ANNUAL INTERNATIONAL SYMPOSIUM ON PERSONAL, INDOOR, AND MOBILE RADIO COMMUNICATIONS (PIMRC)
(2017)