期刊
ESTUARIES AND COASTS
卷 32, 期 6, 页码 1195-1204出版社
SPRINGER
DOI: 10.1007/s12237-009-9211-7
关键词
ELF-97 (R); Flow cytometry; Alkaline phosphatase; Coastal phytoplankton
资金
- EPA Long Island Sound Study [X 98 1613-01-1]
The enzyme-labeled fluorescence (ELF-97A (R)) substrate produces an insoluble, fluorescent yellow-green product at the site of alkaline-phosphatase (AP) activity. Fifteen coastal phytoplankton species were tested to determine if AP activity could be detected in phosphate-depleted media. All species tested, except Synechococcus bacillaris, expressed AP activity. Subsequently, threshold concentrations of soluble reactive phosphate (SRP) at which AP activity could be detected by ELF-97A (R) were determined for Chaetoceros neogracile, Chlorella autotrophica, Isochrysis sp., Prorocentrum minimum, and Tetraselmis chui. Microalgal species differed significantly in the SRP concentration at which AP activity was first detectable (10.1-16.4 A mu M), well above concentrations normally considered limiting for phytoplankton. P. minimum began to express AP activity at a higher SRP concentration than the other algal species; this may be attributable to a relatively high DNA/cell ratio in P. minimum, compared to the other phytoplankton. Thus, phytoplankton species may respond to phosphorus deficiency at high SRP concentrations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据