期刊
JOURNAL OF SEPARATION SCIENCE
卷 39, 期 3, 页码 496-502出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201500915
关键词
Acanthopanax Senticosus; Constituents; Metabolites; Traditional Chinese medicine; Ultra high performance liquid chromatography
资金
- Key Program of Natural Science Foundation of State [81430093, 81173500, 81373930, 81302905, 81202639]
- National Key Subject of Drug Innovation [2015ZX09101043-005, 2015ZX09101043-011]
- Natural Science Foundation of Heilongjiang Province of China [H2015038]
- Youth Innovative Talent Program of Heilongjiang Province of China [UNPYSCT-2015118]
Acanthopanax Senticosus Harms. has been used widely in traditional Chinese medicine for the treatment of chronic bronchitis, neurasthenia, hypertension and ischemic heart disease. However, the in vivo constituents of the stem of Acanthopanax Senticosus remain unknown. In this paper, ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometry and the MarkerLynx (TM) software combined with multiple data processing approach were used to study the constituents in vitro and in vivo. The aqueous extract from the Acanthopanax Senticosus stem and the compositions in rat serum after intragastric administration were completely analyzed. Consequently, 115 compounds in the aqueous extract from Acanthopanax Senticosus stem and 41 compounds absorbed into blood were characterized. Of the 115 compounds in vitro, 54 were reported for first time, including sinapyl alcohol, sinapyl alcohol diglucoside, and 1-O-sinapoyl-beta-Dglucose. In the 41 compounds in vivo, 7 were prototype components and 34 were metabolites which were from 21 components of aqueous extract from Acanthopanax Senticosus stem, and the metabolic pathways of the metabolites were elucidated for first time. The results narrowed the range of screening the active components and provided a basis for the study of action mechanism and pharmacology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据