期刊
EPIDEMIOLOGY AND INFECTION
卷 142, 期 6, 页码 1205-1213出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0950268813002094
关键词
Ehrlichia; epidemiology; PCR; moose; HGA; serology; tick-borne fever
资金
- Swedish Environmental Protection Agency
- SVA
- Environmental Monitoring and Assessment programme
- Faculty of Veterinary Medicine and Animal Science at the Swedish University of Agricultural Sciences
- Wildtech Project [European Union Seventh Framework Programme (FP7)] [222633]
The occurrence of Anaplasma phagocytophilum was investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence of A. phagocytophilum DNA by real-time PCR (n=263), and for Anaplasma antibodies with ELISA serology (n=234). All serum samples had antibodies against A. phagocytophilum. The mean DNA-based prevalence was 26 center dot 3%, and significant (P<0 center dot 01) temporal, and spatial variation was found. Island moose had significantly (P<0 center dot 001) higher prevalence of A. phagocytophilum DNA than moose from the mainland areas. Two samples were sequenced to determine genetic variation in the 16S rRNA and groESL genes. Genetic sequence similarity with the human granulocytic anaplasmosis agent, equine granulocytic ehrlichiosis agent, and different wildlife-associated A. phagocytophilum variants were observed in the 16S rRNA and groESL genes. Our study shows that moose are exposed to A. phagocytophilum in Sweden, and represent a potential wildlife reservoir of the pathogen.
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