Article
Chemistry, Multidisciplinary
Lingfeng Long, Yun Hu, Le Xie, Fubao Sun, Zhenghong Xu, Jinguang Hu
Summary: The study demonstrates a convenient and effective approach for designing a bacterial sensor platform based on bacterial cellulose through CBM2a-assisted cell adhesion strategy, achieving efficient immobilization and sensitive detection of target analyte.
Article
Biotechnology & Applied Microbiology
Sonia Nicchi, Maria Giuliani, Fabiola Giusti, Laura Pancotto, Domenico Maione, Isabel Delany, Cesira L. Galeotti, Cecilia Brettoni
Summary: This study evaluates three approaches for displaying heterologous and homologous lipoproteins on the surface of E. coli, with a focus on the importance of experimental conditions and carrier protein selection in achieving successful protein translocation. The Lpp'OmpA chimera is found to be effective for homologous lipoproteins, while AIDA-I is identified as the best system for mature lipoproteins (especially heterologous ones) without inhibiting growth.
MICROBIAL CELL FACTORIES
(2021)
Review
Microbiology
Gert Bange, Ditlev E. Brodersen, Anastasia Liuzzi, Wieland Steinchen
Summary: Under stressful growth conditions and nutrient starvation, bacteria adapt by synthesizing signaling molecules that profoundly reprogram cellular physiology, with (p)ppGpp playing a key role in this process. The synthesis and hydrolysis of this signaling molecule are carried out by specific enzymes, directly impacting intracellular effectors. Studies have revealed both common features and differences in the effects of (p)ppGpp on gram-negative and gram-positive bacteria.
ANNUAL REVIEW OF MICROBIOLOGY, VOL 75, 2021
(2021)
Article
Biochemistry & Molecular Biology
Aysel Aslanli, Maksim Domnin, Nikolay Stepanov, Elena Efremenko
Summary: The combination of Bacitracin and hexahistidine-containing organophosphate hydrolase (His(6)-OPH) showed improved antibacterial efficiency against both Gram-negative bacteria and yeast strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Ralph Schwidetzky, Max Lukas, Azade YazdanYar, Anna T. Kunert, Ulrich Poschl, Katrin F. Domke, Janine Frohlich-Nowoisky, Mischa Bonn, Thomas Koop, Yuki Nagata, Konrad Meister
Summary: The efficiency of bacterial ice nucleators can be profoundly influenced by ions, with strongly hydrated ions facilitating ice nucleation while weakly hydrated ions inhibiting it. Specific ion-protein interactions on the surface of bacteria play a key role in heterogeneous ice nucleation, highlighting the importance of understanding the nature of ions in this process.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemical Research Methods
Monika Jain, Priyanka Yadav, Bhavana Joshi, Abhijeet Joshi, Prashant Kodgire
Summary: The study focused on the accumulation and exposure of organophosphate pesticides, developing a biosensor based on recombinant organophosphorus hydrolase for sensitive detection using colorimetric and fluorescent methods. The results demonstrate the potential of this biosensor for detecting organophosphate pesticides with good sensitivity and a low limit of detection.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Biotechnology & Applied Microbiology
Yinzhuang Zhu, Yaru Liu, Mingmei Ai, Xiaoqiang Jia
Summary: This study developed a surface display system for carbonic anhydrase using Escherichia coli as the host. The engineered strain showed higher enzyme activity and stability for CO2 capture and mineralization.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Microbiology
Lasse Z. Jensen, Marianne Glasius, Sven-Erik Gryning, Andreas Massling, Kai Finster, Tina Santl-Temkiv
Summary: This study investigates the seasonal variation of bacterial aerosols in the Arctic and finds significant differences in composition and quantity of atmospheric bacterial community between different seasons. In early spring, bacteria mainly originate from temperate and Subarctic regions, while in summer, local sources such as soil, plant material, and melting sea-ice dominate.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Energy & Fuels
Mei Yuin Joanne Wee, Nur Izyan Wan Azelee, Samson Pachelles, Abdul Munir Abd Murad, Farah Diba Abu Bakar, Rosli Md Illias
Summary: In this study, the feasibility of using cell surface display of xylanase on Escherichia coli for the hydrolysis of hemicellulose from pineapple pomace was investigated. The results showed that the application of this technology is possible and offers great potential for the production of xylooligosaccharides in industry.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Plant Sciences
Raghav Kataria, Naveen Duhan, Rakesh Kaundal
Summary: This study elucidates the genome-scale host-pathogen protein-protein interactions between Medicago sativa and Pseudomonas syringae using two computational approaches. Through functional analysis and subcellular localization predictions, new virulence effectors were discovered, providing insights into the pathogen's infection mechanism and aiding in the development of pathogen-resistant lines.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Electrochemistry
Patrick Severin Sfragano, Serena Pillozzi, Ilaria Palchetti
Summary: MicroRNAs and long non-coding RNAs are emerging cancer biomarkers that can be detected using electrochemical and photoelectrochemical biosensors. However, the low abundance of these molecules in biological fluids, especially in early-stage cancer patients, necessitates the use of signal/sample amplification strategies for detection.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Elena Porzio, Maria Rosaria Faraone Mennella, Giuseppe Manco
Summary: DING proteins, with their characteristic N-terminus DINGGG, are widely distributed among different organisms and share a highly conserved phosphate binding site. Despite their sequence conservation, they exhibit heterogeneity in biological activities and have been implicated in various pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Giuseppe Manco, Elena Porzio, Teresa Maria Carusone
Summary: PON1, PON2, and PON3 are a family of lactone hydrolyzing enzymes with varying substrate specificities. Despite originally being thought to primarily defend against pathogens, recent research has highlighted the importance of PON2 in oxidative stress control, inhibition of apoptosis, and cancer progression.
Article
Biochemistry & Molecular Biology
Davide Nardone, Angelo Ciaramella, Mariangela Cerreta, Salvatore Pulcrano, Gian C. Bellenchi, Linda Leone, Giuseppe Manco, Ferdinando Febbraio
Summary: SELDI-TOF mass spectrometry is used for protein analysis and biomarker screening, with SELYMATRA being a web application developed to automate identification processes and predict proteins in complex mixtures.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Gina Elena Giacomazzo, Luca Conti, Annalisa Guerri, Marco Pagliai, Camilla Fagorzi, Patrick Severin Sfragano, Ilaria Palchetti, Giangaetano Pietraperzia, Alessio Mengoni, Barbara Valtancoli, Claudia Giorgi
Summary: This study successfully tuned the mode of action of nitroimidazole derivatives by synthesizing different architectures of ruthenium complexes, and improved their antibacterial activity upon light exposure. This is of significant importance for the design of novel antimicrobials and their application in biologically relevant environments.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Andreia C. M. Rodrigues, Maria Vittoria Barbieri, Marco Chino, Giuseppe Manco, Ferdinando Febbraio
Summary: This study aimed to evaluate the applicability of Forster resonance energy transfer (FRET) as a sensitive and reliable method to quantify organophosphate (OP) pesticides at environmentally relevant concentrations. The results showed that fluorometric measurements using an enzyme-based biosensor with FRET can linearly quantify different concentrations of OP pesticides and are not interfered by changes in the pH of the medium or the addition of other organic components.
Article
Multidisciplinary Sciences
Teresa Maria Carusone, Giuseppe Manco
Summary: This study discovered the regulatory effects of post-translational modifications and single nucleotide polymorphisms on the activity of human lactonase Paraoxonase 2 (PON2), and confirmed the symmetry of the molecule through structural analysis. This finding is of great significance for understanding the symmetry evolution and function of biological molecules.
Article
Chemistry, Analytical
Valentina Pifferi, Anna Testolin, Chiara Ingrosso, Maria Lucia Curri, Ilaria Palchetti, Luigi Falciola
Summary: By synthesizing a hybrid nanocomposite composed of functionalized reduced graphene oxide sheets and Au nanoparticles, high electrochemical activity and sensing ability for As(III) ions were achieved. The results of this study demonstrate the potential of the nanocomposite as an effective technique for detecting heavy metals in the environment.
Article
Biochemical Research Methods
Patrick Severin Sfragano, Serena Laschi, Lapo Renai, Michelangelo Fichera, Massimo Del Bubba, Ilaria Palchetti
Summary: The article introduces a new method of using biochar derived from biological sludge to prepare a carbon paste electrode and investigates its physicochemical properties. The experimental results show that biochar can be a promising material for electroanalytical sensors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Giuseppe Manco, Giuseppina Lacerra, Elena Porzio, Giuliana Catara
Summary: Cellular functions are regulated through the gene expression program, including transcription of mRNA, alternative RNA splicing, and protein synthesis. Post-translational modifications of proteins, such as ADP-ribosylation, add another layer of complexity to the regulatory network. ADP-ribosylation plays a crucial role in various cellular processes, including DNA damage repair, transcriptional regulation, and cell survival. ADP-ribosyltransferases (ARTs), as enzymes involved in ADP-ribosylation, are emerging as potential drug targets. This review provides an overview of human ARTs and their biological functions, with a focus on the regulation of RNA functions by ADP-ribosyltransferase Diphtheria toxin-like enzymes (ARTD).
Article
Biochemistry & Molecular Biology
Adriano Pasqui, Anna Boddi, Domenico Andrea Campanacci, Guido Scoccianti, Andrea Bernini, Daniela Grasso, Elisabetta Gambale, Federico Scolari, Ilaria Palchetti, Annarita Palomba, Sara Fancelli, Enrico Caliman, Lorenzo Antonuzzo, Serena Pillozzi
Summary: In this study, we observed a severe deregulation of the nucleotide excision repair (NER) pathway in soft tissue sarcoma (STS) patients and described the effect of SNP rs1047768 in the ERCC5 structure for the first time. These findings revealed the correlation between specific genotypes of ERCC genes and the proficiency of the NER pathway in STS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Analytical
Claudia Patiti, Patrick Severin Sfragano, Serena Laschi, Serena Pillozzi, Anna Boddi, Olivia Crociani, Andrea Bernini, Ilaria Palchetti
Summary: The determination of nucleic acids has many applications in various fields, and PCR-based methods are considered the gold standards. Combining isothermal amplification methods with electrochemical biosensing techniques allows for the development of lab-on-a-chip tools and wearable sensors with advantages such as simplicity, rapidity, low energy consumption, and multi-analyte detection capability.
Article
Biochemistry & Molecular Biology
Luca Conti, Gina Elena Giacomazzo, Barbara Valtancoli, Mauro Perfetti, Alberto Privitera, Claudia Giorgi, Patrick Severin Sfragano, Ilaria Palchetti, Sara Pecchioli, Paola Bruni, Francesca Cencetti
Summary: This study investigated the anti-cancer effect of highly charged Ru(II)-polypyridyl complexes as photosensitizers in photodynamic therapy. These complexes efficiently produced singlet oxygen and reactive oxygen species, leading to programmed cell death and anti-tumor activity in ovarian cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Valentina De Luca, Luigi Mandrich, Giuseppe Manco
Summary: In recent decades, the increased use of pesticides in agriculture has led to safety concerns for the environment and human health, necessitating the development of new detection and decontamination systems. A new qualitative test utilizing thermostable enzymes was discovered, allowing for the detection of pesticide residues on fruits and vegetables. By analyzing esterase activity, the presence of residual pesticides and their decontamination was successfully assessed.
Article
Materials Science, Multidisciplinary
Chiara Ingrosso, Michela Corricelli, Anna Testolin, Valentina Pifferi, Francesca Bettazzi, Giuseppe V. Bianco, Nicoletta Depalo, Elisabetta Fanizza, Marinella Striccoli, Ilaria Palchetti, M. Lucia Curri, Luigi Falciola
Summary: A novel colloidal hybrid nanocomposite has been synthesized through a facile and cost-effective in situ colloidal procedure. The nanocomposite exhibits good dispersibility and high electrical conductivity, and shows superior performance in the detection of biomarkers such as dopamine. It holds great potential for medical applications.
MATERIALS ADVANCES
(2023)
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Summary: Methylation plays important roles in various biological processes, and this study characterized the function of ThnM3 in regioselective modifications of 1,8-dihydroxynaphthalene.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
Summary: In this study, a highly sensitive and specific method using ultra performance liquid chromatography (UPLC) was developed to measure the enzymatic activity of APH4 in plants. The method exhibited good precision and accuracy, and was validated for use with samples from different sources.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Cuixia Zhou, Guangcheng Yang, Panpan Meng, Weishuai Qin, Yanyan Li, Zhenxian Lin, Wei Hui, Huitu Zhang, Fuping Lu
Summary: Bacillus licheniformis 2709 is an important industrial producer of alkaline protease (AprE), and this study identified the UP elements of the aprE gene and demonstrated the lack of significant effect of CggR, FruR, and YhcZ transcription factors on aprE expression. Enhanced aprE expression and enzyme activity were achieved by deleting a negative regulatory factor binding site in the genome. These findings provide valuable insights into the high-level production of proteins in other Bacillus species.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Summary: In this study, laccase was immobilized on modified magnetic nanoparticles, improving the enzyme's thermostability and efficiency for polymerization of phenols.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Handan Ustukarci, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
Summary: A biofilm layer called biofouling is formed over time by organic molecules and microorganisms on solid surfaces immersed in a liquid medium. This study focused on the antifouling performance of polyaniline (PANI) and investigated the immobilization of hydrolytic enzymes in PANI coatings. The results showed that the alpha-amylase-containing film exhibited the highest antifouling activity. Surface properties and biofilm composition were analyzed to support these findings.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo Jose Inocencio-Garcia, Carlos Ariel Cardona Alzate
Summary: This study evaluated the sustainability of biorefineries using orange peel as a raw material. By applying the concept of biorefineries and experimental data, it was found that steam distillation and polyphenolic compound extraction can improve the process performance, resulting in higher yields of different products. The integrated utilization of orange peel in biorefineries showed a high sustainability index.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)