Article
Energy & Fuels
Maria Augusta de Carvalho Silvello, Julian Martinez, Rosana Goldbeck
Summary: This study assessed the effect of supercritical carbon dioxide (SC-CO2) on the enzymatic hydrolysis of sugarcane bagasse to produce fermentable sugars. The optimal conditions for SC-CO2 treatment were determined to be 50°C, 200 bar, and 120 min, resulting in the highest concentration of reducing sugars and hydrolysis yield.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Chemistry, Applied
Gabriela Bonan Hermsdorff, Estephanie Laura Nottar Escobar, Thiago Alessandre da Silva, Arion Zandona Filho, Marcos Lucio Corazza, Luiz Pereira Ramos
Summary: This study utilized organic acid catalysts and supercritical CO(2)-assisted organosolv pretreatment to efficiently remove lignin and retain glucose from sugarcane bagasse. The optimized conditions achieved high delignification and glucan retention rates. Additionally, it was found that organic acid esterification was a limiting factor at high ethanol titer.
CARBOHYDRATE POLYMERS
(2023)
Article
Materials Science, Paper & Wood
Pratibha Baral, Meghana Munagala, Yogendra Shastri, Vinod Kumar, Deepti Agrawal
Summary: The study focused on reducing the production cost of high gravity sugar solutions from sugarcane bagasse by optimizing pretreatment and hydrolysis processes, evaluating two alternative strategies to minimize the requirement of cellulase enzyme blend, and achieving higher sugar conversion yields.
Article
Green & Sustainable Science & Technology
Meenu Hans, Shruti Garg, Vanessa O. A. Pellegrini, Jefferson G. Filgueiras, Eduardo R. de Azevedo, Francisco E. C. Guimaraes, Anuj Kumar Chandel, Igor Polikarpov, Bhupinder Singh Chadha, Sachin Kumar
Summary: The study optimized the conditions for liquid ammonia pretreatment of sugarcane bagasse and achieved a high yield of reducing sugars during enzymatic hydrolysis. The efficiency of the process was further validated through biophysical techniques, confirming the structural and compositional changes of the biomass.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Physical
I-Hsiang Tseng, Bo-Ju You, Po-Ya Chang
Summary: This study demonstrates a strategy to utilize sugarcane bagasse as a catalyst support for the conversion of carbon dioxide into value-added products, such as carbon monoxide or methanol, under ambient conditions.
CATALYSIS COMMUNICATIONS
(2022)
Article
Agricultural Engineering
Aissata Ousmane Kane, Anelyse Abreu Cortez, Vanessa Oliveira Arnoldi Pellegrini, Balla Diop Ngom, Jefferson G. Filgueiras, Eduardo R. de Azevedo, Igor Polikarpov
Summary: Sugarcane bagasse was pretreated with a combination of liquid hot water and neutral/alkaline sulfonation at low temperatures to enhance enzymatic hydrolysis yields. The combination of liquid hot water pretreatment and sulfonation resulted in excellent enzymatic hydrolysis yields.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agricultural Engineering
Qibo Zhang, Yujie Zhai, Shuangquan Yao, Rong Huang, Xin Zhou, Kankan Jiang
Summary: This study used a combination of hydrothermal and endo-xylanase hydrolysis methods to enhance xylooligosaccharide (XOS) yields, achieving a maximum XOS of 54.5% with low degree of polymerization. Acidic hydrolysis using dilute sulfuric acid was also employed to improve the saccharification efficiency of cellulose. The sequential hydrothermal-endo-xylanase-acid-cellulase hydrolysis process effectively co-produced biomass-derived sugar products, increasing the value of sugarcane bagasse.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agricultural Engineering
Leping Zhang, Yuejie Qiu, Fuhou Lei, Pengfei Li, Jianxin Jiang
Summary: In this study, acidic amino acid glutamic acid was used to pretreat sugarcane bagasse for the production of xylo-oligosaccharides (XOS) and glucose. The use of glutamic acid not only increased the efficiency of enzymes, but also increased the pore size and porosity of sugarcane bagasse.
BIORESOURCE TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Shreya Anand, Sampath Muthu Kumar, Koel Mukherjee, Padmini Padmanabhan
Summary: This paper develops a technological scheme to produce 2G fermentable sugar from sugarcane bagasse and assesses its feasibility. The study includes recovery of fermentable sugar, characterization of pretreated hydrolysed bagasse, in-silico elucidation of enzymatic hydrolysis, and techno-economic analysis of sugar recovery from bagasse.
JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE
(2022)
Review
Energy & Fuels
Ghulam Mustafa, Muhammad Arshad, Ijaz Bano, Mazhar Abbas
Summary: Sugarcane bagasse and sugar beet molasses can be used as low-cost energy sources for microbial growth and production of microbial metabolites. They are gaining momentum as alternative substrates for the manufacture of high valued products and can greatly contribute to human sustainability.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Soil Science
Barbara Samartini Queiroz Alves, Katherin Prissila Sevilla Zelaya, Fernando Colen, Ledivan Almeida Frazao, Alfredo Napoli, Sanjai J. Parikh, Luiz Arnaldo Fernandes
Summary: Biochar has been studied as an alternative for waste disposal and a source of nutrients for soil amendment, with potential for carbon sequestration. The use of biochar from sewage sludge (SB) and a mixture of sewage sludge and sugarcane bagasse (MB) affected soil properties, sugar beet production, and trace element concentrations in soil. Overall, biochar treatments increased soil fertility and carbon stock, but also led to higher concentrations of trace elements in both soil and plants.
Article
Biochemistry & Molecular Biology
Eva Balaguer Moya, Berta Syhler, Julen Ordenana Manso, Giuliano Dragone, Solange I. Mussatto
Summary: This study evaluated the optimization of enzymatic hydrolysis cocktail to maximize sugar extraction from pretreated sugarcane bagasse. It was found that adding hydrogen peroxide at the beginning of hydrolysis increased glucose and xylose concentrations, while the addition of hemicellulase increased glucose and xylose production. These findings suggest that using an appropriate enzymatic cocktail supplemented with additives can enhance sugar extraction from lignocellulosic biomass and contribute to the development of a more sustainable and competitive process.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Wuhuan Li, Xuesong Tan, Changlin Miao, Zhanying Zhang, Yunxuan Wang, Arthur J. Ragauskas, Xinshu Zhuang
Summary: This study utilized acetone/phenoxyethanol/water (APW) solutions to pretreat sugarcane bagasse and improve sugar production in enzymatic hydrolysis. The optimal pretreatment conditions were determined to be 125 degrees C-120 min, 0.17 M H2SO4, and a liquid-solid ratio of 15, resulting in high lignin removal and cellulose digestibility. The APW process also showed effective fractionation of other biomass feedstocks, such as pine, corn stalk, and bamboo.
Article
Agricultural Engineering
Zhen Zhu, Rong Huang, Shuangquan Yao, Yiruo Liu, Qibo Zhang, Xin Zhou, Kankan Jiang
Summary: In this study, sugarcane bagasse was pretreated using a renewable organic acid (xylonic acid) derived from the biooxidation of xylose. The effects of reaction temperature and time on the release of fermentable xylose and glucose were investigated. After optimization, a pretreatment with 1% xylonic acid at 190 degrees C for 30 min was selected, resulting in a release of 70% xylose and an enzymatic hydrolysis yield of 86.5%. The pretreated hydrolysate liquor was also shown to be suitable for xylonate production through the biooxidation of Gluconobacter oxydans. Additionally, the sequential process of xylonic acid pretreatment and saccharification provides a pathway for recycling lignin as value-added bioproducts. Overall, this study presents a green-like strategy for fully utilizing sugarcane bagasse.
BIORESOURCE TECHNOLOGY
(2023)
Article
Energy & Fuels
Armando Navarro, Carmina Montiel, Jesus Gracia-Fadrique, Alberto Tecante, Eduardo Barzana
Summary: Supercritical carbon dioxide (SCCO2) pretreatment of blue agave bagasse (BAB) enhances enzymatic hydrolysis (EH) to simple sugars for bioethanol fermentation.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Cardiac & Cardiovascular Systems
Cristine Schmidt, Daniel Moreira-Goncalves, Mario Santos, Adelino Leite-Moreira, Jose Oliveira
Summary: Exercise training can improve symptoms, quality of life, and cardiac function in HFpEF patients, but its effect on mortality and hospitalization needs further investigation.
HEART FAILURE REVIEWS
(2022)
Review
Endocrinology & Metabolism
Giorjines Boppre, Florencio Diniz-Sousa, Lucas Veras, Jose Oliveira, Helder Fonseca
Summary: Exercise, combined exercise regimens, and interventions starting more than 6 months after bariatric surgery were effective in promoting reductions in body weight, waist circumference, and body mass index. Exercise following bariatric surgery did not appear to favor improvements in body composition.
OBESITY SCIENCE & PRACTICE
(2022)
Article
Integrative & Complementary Medicine
Gabriela Sabrina Anzollin, Lilian Zaki, Thalita Maria Perin, Bruna Finger, Leticia Trentin Perin, Fernanda Petry, Thalia Sebulsqui Saraiva, Maria Luiza Lima da Costa Lopes, Andresa Heemann Betti, Jaqueline Scapinello, J. Vladimir Oliveira, Jacir Dal Magro, Liz G. Muller
Summary: The extract obtained from Campomanesia xanthocarpa seeds using supercritical CO2 exhibits antidepressant-like activity and its mechanism of action may involve the activation of monoaminergic neurotransmission (serotonergic, dopaminergic, and noradrenergic).
JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE
(2022)
Correction
Surgery
Florencio Diniz-Sousa, Giorjines Boppre, Lucas Veras, Alba Hernandez-Martinez, Jose Oliveira, Helder Fonseca
Review
Surgery
Florencio Diniz-Sousa, Giorjines Boppre, Lucas Veras, Alba Hernandez-Martinez, Jose Oliveira, Helder Fonseca
Summary: The study aimed to assess the effect of exercise on bone mineral density after bariatric surgery. Systematic search found that exercise had a positive effect on BMD at the total hip, femoral neck, lumbar spine, and 1/3 radius. However, the certainty of evidence was low.
Article
Chemistry, Applied
George F. Finco, Karina G. Fiametti, Viviane da Silva Lobo, Edson A. da Silva, Fernando Palu, Joao H. C. Wancura, Maria L. F. Rodrigues, Alexsandra Valerio, Jose Vladimir de Oliveira
Summary: In this study, enzymatic glycerolysis of olive oil was used to synthesize MAG and DAG. Experiment results showed that under the conditions of 35 degrees C, 7.5% Lipozyme TL 100L and a glycerol to oil volumetric ratio of 2:1, the conversion of TAG reached approximately 98%.
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2022)
Article
Sport Sciences
Lucas Veras, Florencio Diniz-Sousa, Giorjines Boppre, Ana Resende-Coelho, Edgar Moutinho-Ribeiro, Vitor Devezas, Hugo Santos-Sousa, John Preto, Joao Paulo Vilas-Boas, Leandro Machado, Jose Oliveira, Helder Fonseca
Summary: This study developed accelerometry-based equations to predict pGRF and pLR during walking and running, and compared the predicted values with actual values, demonstrating high accuracy of these equations.
EUROPEAN JOURNAL OF SPORT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Mirian Cristina Feiten, Iasmin Morigi, Marco Di Luccio, Jose Vladimir Oliveira
Summary: The aim of this study was to investigate the treatment of Candida antarctica Lipase B (CalB) in supercritical carbon dioxide (SC-CO2) and liquefied petroleum gas (LPG), as well as its activity after high-pressure treatment. The results showed that CalB treated with SC-CO2 at 35 ℃, 75 bar for 1 hour and LPG at 65 ℃, 30 bar for 1 hour had residual specific hydrolytic activities of 132% and 142%, respectively. The enzyme treated at high pressure still maintained an activity above 100% after 20 days of storage at low temperatures. There was no significant difference in the residual activity loss of CalB treated with LPG and stored at different temperatures over time. However, a greater difference was observed between CalB treated with CO2 and flash-frozen in liquid nitrogen (-196 ℃) followed by storage in a freezer (-10 ℃) compared to CalB stored in a freezer at -10 ℃. These findings suggest the need for further studies on CalB and other enzymes under different pressurized fluids for industrial applications.
BIOTECHNOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Juciane Prois Fortes, Fernanda Wouters Franco, Julia Baranzelli, Gustavo Andrade Ugalde, Cristiano Augusto Ballus, Eliseu Rodrigues, Marcio Antonio Mazutti, Sabrina Somacal, Claudia Kaehler Sautter
Summary: Consumers increasingly prefer functional beverages, and in this study, we developed and characterized a novel functional beverage by combining the health benefits of honey with the traditional aging process in wood. The addition of oak chips during aging significantly impacted the phenolic compounds, flavonoids, and antioxidant capacity of the mead. Eighteen compounds, including organic acids, phenolic acids, flavonoids, and tannins, were identified in the meads after 360 days.
Article
Pharmacology & Pharmacy
Patricia Viera de Oliveira, Otavio Sanaiotto, Ketelin Zamin Kuhn, Amanda Oltramari, Adailton J. Bortoluzzi, Marcelo Lanza, Gean Pablo S. Aguiar, Anna Maria Siebel, Liz Girardi Muller, Vladimir Oliveira
Summary: Microparticles of naringenin were successfully obtained using the gas antisolvent technique, and their potential for treating positive symptoms of schizophrenia was evaluated using a mouse model. The results showed that micronized naringenin exhibited a higher dissolution rate and bioavailability, indicating its potential as an antipsychotic agent.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bruna P. Soares, Ana M. Ferreira, Marina Justi, Luiz Gustavo Goncalves Rodrigues, J. Vladimir Oliveira, Simao P. Pinho, Joao A. P. Coutinho
Summary: Anthocyanins from jucara fruits were extracted using biobased solvents, 1,2-alkanediols and glycerol ethers, through either pressurized liquid extraction (PLE) or ultrasound-assisted extraction (UAE). The optimized PLE conditions resulted in 23.1 mg(anthocyanins)center dot g(dry biomass)(-1), while the UAE conditions with 1,2-propanediol yielded 50 mg(anthocyanins)center dot g(dry biomass)(-1). The biobased solvent solutions exhibited higher anthocyanin yields and antioxidant capacity compared to water and ethanolic extracts.
Article
Chemistry, Analytical
Lucas Veras, Florencio Diniz-Sousa, Giorjines Boppre, Vitor Devezas, Hugo Santos-Sousa, John Preto, Joao Paulo Vilas-Boas, Leandro Machado, Jose Oliveira, Helder Fonseca
Summary: The purpose of this study was to develop prediction equations for peak ground reaction force (pGRF) and peak loading rate (pLR) in high-impact activities for adults with various body masses. Regression equations were developed using accelerometry data, with body mass and peak acceleration or acceleration rate as predictors. The equations had high accuracy for predicting pGRF, with a coefficient of determination (R-2) of at least 0.83 and a mean absolute percentage error (MAPE) below 14.5%. However, the accuracy of the pLR prediction equations was lower than that of the pGRF equations.
Article
Environmental Sciences
Ruan F. F. Vieira, Eduardo B. B. Sydney, Karina G. G. Fiametti, Joao H. C. Wancura, J. Vladimir Oliveira
Summary: This study investigated the production of volatile fatty acids (VFAs) by a consortium of microorganisms obtained from whey. The effectiveness of chemical hydrolysis and supplementation with yeast extract and peptone was evaluated to determine the process that provides the highest VFAs production. The results showed that chemical hydrolysis did not significantly affect the production of compounds, while supplementation played a crucial role in increasing VFAs production. Butyric acid was the compound with the highest amount produced, and it had better results with supplemented hydrolyzed whey.
WASTE AND BIOMASS VALORIZATION
(2023)
Review
Energy & Fuels
Eulalia L. S. Barros, Evertan A. Rebelatto, Diego A. Mayer, Joao H. C. Wancura, J. Vladimir Oliveira
Summary: Sugar esters (SE) are amphiphilic compounds with environmentally friendly features, including non-toxicity, odorless, tasteless, biodegradability, and various functions. The use of lipases as biocatalysts for SE production offers advantages such as reduced energy consumption, higher selectivity, and less use of harmful solvents. Supercritical fluids, especially carbon dioxide, are attractive solvents for SE production due to their unique properties. However, there are challenges in terms of operating conditions and costs. Future research should focus on developing cost-effective bioreactors and enhancing lipases' stability. Rating: 8
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2023)
Article
Biochemistry & Molecular Biology
Suiane Cavalcante, Sofia Viamonte, Rui S. Cadilha, Ilda P. Ribeiro, Ana Cristina Goncalves, Joao Sousa-Venancio, Marisol Gouveia, Manuel Teixeira, Mario Santos, Jose Oliveira, Fernando Ribeiro
Summary: This study aims to evaluate the effects of a single exercise bout on the circulating levels of EPCs and CECs in heart failure patients. The results showed that a single exercise bout increased the levels of EPCs but didn't change the levels of CECs. Heart failure patients had lower levels of EPCs compared to the age-matched group, but the exercise bout restored their EPCs levels to a level similar to the age-matched group.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Summary: Methylation plays important roles in various biological processes, and this study characterized the function of ThnM3 in regioselective modifications of 1,8-dihydroxynaphthalene.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
Summary: In this study, a highly sensitive and specific method using ultra performance liquid chromatography (UPLC) was developed to measure the enzymatic activity of APH4 in plants. The method exhibited good precision and accuracy, and was validated for use with samples from different sources.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Cuixia Zhou, Guangcheng Yang, Panpan Meng, Weishuai Qin, Yanyan Li, Zhenxian Lin, Wei Hui, Huitu Zhang, Fuping Lu
Summary: Bacillus licheniformis 2709 is an important industrial producer of alkaline protease (AprE), and this study identified the UP elements of the aprE gene and demonstrated the lack of significant effect of CggR, FruR, and YhcZ transcription factors on aprE expression. Enhanced aprE expression and enzyme activity were achieved by deleting a negative regulatory factor binding site in the genome. These findings provide valuable insights into the high-level production of proteins in other Bacillus species.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Summary: In this study, laccase was immobilized on modified magnetic nanoparticles, improving the enzyme's thermostability and efficiency for polymerization of phenols.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Handan Ustukarci, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
Summary: A biofilm layer called biofouling is formed over time by organic molecules and microorganisms on solid surfaces immersed in a liquid medium. This study focused on the antifouling performance of polyaniline (PANI) and investigated the immobilization of hydrolytic enzymes in PANI coatings. The results showed that the alpha-amylase-containing film exhibited the highest antifouling activity. Surface properties and biofilm composition were analyzed to support these findings.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo Jose Inocencio-Garcia, Carlos Ariel Cardona Alzate
Summary: This study evaluated the sustainability of biorefineries using orange peel as a raw material. By applying the concept of biorefineries and experimental data, it was found that steam distillation and polyphenolic compound extraction can improve the process performance, resulting in higher yields of different products. The integrated utilization of orange peel in biorefineries showed a high sustainability index.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
