期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 48, 期 4-5, 页码 416-426出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2011.02.001
关键词
Biomimetic; CO2-sequestration; Immobilization; Carbonic anhydrase; P. fragi; Indigenous
资金
- DBT, New Delhi
- Council of Scientific and Industrial Research (CSIR), New Delhi
The present study deals with immobilization of purified CA and whole cell of Pseudomonas fragi, Micrococcus lylae, and Micrococcus luteus 2 on different biopolymer matrices. Highest enzyme immobilization was achieved with P. fragi CA (89%) on chitosan-KOH beads, while maximum cell immobilization was achieved with M. lylae (75%) on chitosan-NH4OH beads. A maximum increase of 1.08-1.18 fold stability between 35 and 55 degrees C was observed for M. lylae immobilized CA. The storage stability was improved by 2.02 folds after immobilization. FTIR spectra confirmed the adsorption of CA on chitosan-KOH beads following hydrophilic interactions. Calcium carbonate precipitation was achieved using chitosan-KOH immobilized P. fragi CA. More than 2 fold increase in sequestration potential was observed for immobilized system as compared to free enzyme. XRD spectra revealed calcite as the dominant phase in biomimetically produced calcium carbonate. (C) 2011 Elsevier Inc. All rights reserved.
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