Article
Microbiology
Matthew P. Hirakawa, Alberto Rodriguez, Mary B. Tran-Gyamfi, Yooli K. Light, Salvador Martinez, Henry Diamond-Pott, Blake A. Simmons, Kenneth L. Sale
Summary: Phlebia radiata is a fungus that has the ability to degrade aromatic compounds, xenobiotics, and lignin using laccase enzymes. A study found that phenothiazines, especially promethazine, can induce laccase expression and enhance the lignin degradation capabilities of P. radiata. Additionally, promethazine treatment also increased laccase activity in other lignin-converting fungi.
Article
Biotechnology & Applied Microbiology
Tangwu Cui, Bo Yuan, Haiwei Guo, Hua Tian, Weimin Wang, Yingqun Ma, Changzhi Li, Qiang Fei
Summary: This study utilized a consortium of white rot fungi to enhance ligninolytic enzyme activity and achieved a 50% lignin degradation rate. The enzymatic activity from the consortium was significantly improved compared to monocultures, indicating a potential bioconversion route for lignin utilization.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Multidisciplinary Sciences
Herman Suryadi, Jessica J. Judono, Merianda R. Putri, Alma D. Eclessia, Jiihan M. Ulhaq, Dinar N. Agustina, Triyani Sumiati
Summary: This review explores the applications of enzymes in lignocellulose research, including the preparation methods, factors affecting enzyme activity, and potential future prospects.
Article
Materials Science, Paper & Wood
Libo Jin, Guoming Zeng, Haojie Chen, Lei Wang, Hao Ji, Sue Lin, Renyi Peng, Da Sun
Summary: The mechanism of lignin degradation by white rot fungi was studied using Phanerochaete chrysosporium and Pleurotus ostreatus. The removal of alkali lignin was mainly through precipitation and enzymolysis, with enzymolysis being dependent on the lignin concentration and enzyme activity.
Article
Agricultural Engineering
Jiyun Qi, Lu Jia, Yanjun Liang, Bei Luo, Rurong Zhao, Chen Zhang, Jialong Wen, Yonghui Zhou, Mizi Fan, Yan Xia
Summary: This study investigated the biodegradation abilities of different fungi on Dendrocalamus sinicus and studied the changes in chemical characteristics of the bamboo. The results showed that white-rot fungi T. versicolor selectively degraded the bamboo, while brown-rot fungi G. trabeum and R. placenta mainly attacked it during the middle stage. These findings have important implications for the bioconversion of Dendrocalamus sinicus.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Biotechnology & Applied Microbiology
Daniela Chmelova, Barbora Legerska, Jana Kunstova, Miroslav Ondrejovic, Stanislav Miertus
Summary: This review provides an overview of laccase production by white-rot fungi under solid-state fermentation conditions. The variations in the up-stream, fermentation, and down-stream processes and their impact on laccase activity are discussed. The studies suggest that a suitable combination of lignocellulosic material and laccase producer can shorten the production process, and the utilization of waste materials can reduce costs and increase competitiveness.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xinping Liu, Wei Deng, Yang Yang
Summary: "The study successfully purified a laccase LAC-Yang1 from a high laccase activity white-rot fungus Pleurotus ostreatus strain yang1. The LAC-Yang1 demonstrated strong tolerance to acidic and alkaline conditions, as well as various inhibitors, metal ions, and organic solvents. It exhibited efficient degradation and detoxification of chlorophenols, highlighting its potential in environmental biotechnology."
Article
Materials Science, Multidisciplinary
Murat Uygun, Victor de la Asuncion-Nadal, Sinem Evli, Deniz Akta Uygun, Beatriz Jurado-Sanchez, Alberto Escarpa
Summary: Laccase-based micromotors were synthesized and used for decolorization of azo dyes in textile wastewater, showing improved dye removal capacity compared to the free enzyme approach. The stability of the immobilized enzyme was enhanced, resulting in higher decolorization efficiencies of 76-94% compared to 34-55% using free enzyme.
APPLIED MATERIALS TODAY
(2021)
Article
Microbiology
Jing Si, Hongfei Ma, Yongjia Cao, Baokai Cui, Yucheng Dai
Summary: This study introduces a valuable laccase, ThLacc-S, which efficiently metabolizes endocrine disrupting chemicals and has high specific activity. The enzyme maintains stability in a wide temperature range and pH range, making it a promising green catalyst for various biotechnological and industrial applications.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Shuqi Lin, Jinchao Wei, Bentao Yang, Meng Zhang, Rui Zhuo
Summary: This paper reviews the role of white rot fungal CYP450 in the biodegradation of organic pollutants, focusing on inhibitory experiments and direct/indirect evidence. It also discusses the catalytic mechanisms of white rot fungal CYP450, its application potential, and future prospects for bioremediation.
Article
Green & Sustainable Science & Technology
Carolina Velasquez-Quintero, Andres Merino-Restrepo, Angelina Hormaza-Anaguano
Summary: This study evaluated the production of laccase enzyme by four white-rot fungi and optimized the fermentation conditions to maximize enzyme yield.
JOURNAL OF CLEANER PRODUCTION
(2022)
Review
Environmental Sciences
Rui Zhuo, Fangfang Fan
Summary: White rot fungi have shown promising results in the remediation of organic pollutants, thanks to their unique oxidative and ligninolytic systems. This review summarizes advancements in the use of white rot fungi for bioremediation purposes over the past decade, while highlighting potential research areas and trends in the field.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Junko Sugano, Ndegwa Maina, Janne Wallenius, Kristiina Hilden
Summary: Wood decomposition is a complex process involving various biocatalysts working synergistically to break down plant cell walls efficiently. The interaction of fungal communities during wood decay plays a crucial role in the degradation process. Different combinations of white-rot and brown-rot fungi have shown varying enzymatic activities and degradation efficiency on different woody substrates.
Review
Environmental Sciences
Heli Patel, Virendra Kumar Yadav, Krishna Kumar Yadav, Nisha Choudhary, Haresh Kalasariya, M. Mujahid Alam, Amel Gacem, Mohammed Amanullah, Hala A. Ibrahium, Jae-Woo Park, Sungmin Park, Byong-Hun Jeon
Summary: The textile industry produces synthetic dyestuffs that pose a threat to the environment, with azo dyes being the most commonly used. Current effluent treatment methods cannot effectively remove these dyes, but bacteria can decolourize and degrade them. However, textile plant wastewater can contain other pollutants and dyes. This study aims to fill the knowledge gap in dye remediation and provide the latest trends, which is important for industries and researchers involved in dyestuffs.
Article
Biotechnology & Applied Microbiology
Yakath Ali Nurul-Aliyaa, Nik Azimatolakma Awang, Masratul Hawa Mohd
Summary: This study aimed to isolate and identify white rot fungi from wood decayed and evaluate their ability to produce lignin-modifying enzymes. The results showed that several fungal isolates can produce different types of enzymes, with Phanerochaete australis and Lentinus squarrosulus exhibiting the highest activity.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Ana S. Luis, Jonathon Briggs, Xiaoyang Zhang, Benjamin Farnell, Didier Ndeh, Aurore Labourel, Arnaud Basle, Alan Cartmell, Nicolas Terrapon, Katherine Stott, Elisabeth C. Lowe, Richard McLean, Kaitlyn Shearer, Julia Schuckel, Immacolata Venditto, Marie-Christine Ralet, Bernard Henrissat, Eric C. Martens, Steven C. Mosimann, D. Wade Abbott, Harry J. Gilbert
NATURE MICROBIOLOGY
(2018)
Article
Agriculture, Multidisciplinary
Stephanie Rollero, Anscha J. J. Zietsman, Fanny Buffetto, Julia Schuckel, Anne Ortiz-Julien, Benoit Divol
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2018)
Article
Microbiology
Lindsey M. Solden, Adrian E. Naas, Simon Roux, Rebecca A. Daly, William B. Collins, Carrie D. Nicora, Sam O. Purvine, David W. Hoyt, Julia Schuckel, Bodil Jorgensen, William Willats, Donald E. Spalinger, Jeffrey L. Firkins, Mary S. Lipton, Matthew B. Sullivan, Phillip B. Pope, Kelly C. Wrighton
NATURE MICROBIOLOGY
(2018)
Article
Chemistry, Applied
Gonzalo Garrido-Banuelos, Astrid Buica, Julia Schuckel, Anscha J. J. Zietsman, William G. T. Willats, John P. Moore, Wessel J. Du Toit
Article
Chemistry, Applied
Gonzalo Garrido-Banuelos, Astrid Buica, Julia Schuckel, Anscha J. J. Zietsman, William G. T. Willats, John P. Moore, Wessel J. Du Toit
Article
Biotechnology & Applied Microbiology
Davin L. Williams, Julia Schueckel, Melane A. Vivier, Fanny Buffetto, Anscha J. J. Zietsman
BIOCHEMICAL ENGINEERING JOURNAL
(2019)
Article
Food Science & Technology
Andrea Osete-Alcaraz, Encarna Gomez-Plaza, Pilar Martinez-Perez, Florent Weiller, Julia Schuckel, William G. T. Willats, John P. Moore, Jose M. Ros-Garcia, Ana B. Bautista-Ortin
FOOD RESEARCH INTERNATIONAL
(2020)
Article
Plant Sciences
Pablo Ric-Varas, Marta Barcelo, Juan A. Rivera, Sergio Cerezo, Antonio J. Matas, Julia Schuckel, J. Paul Knox, Sara Pose, Fernando Pliego-Alfaro, Jose A. Mercado
Article
Plant Sciences
Candelas Paniagua, Pablo Ric-Varas, Juan A. Garcia-Gago, Gloria Lopez-Casado, Rosario Blanco-Portales, Juan Munoz-Blanco, Julia Schuckel, J. Paul Knox, Antonio J. Matas, Miguel A. Quesada, Sara Pose, Jose A. Mercado
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Biochemistry & Molecular Biology
Andrea Osete-Alcaraz, Encarna Gomez-Plaza, Pilar Martinez-Perez, Florent Weiller, Julia Schuckel, William G. T. Willats, John P. Moore, Jose M. Ros-Garcia, Ana B. Bautista-Ortin
Summary: The study found that hydrolytic enzymes, especially cellulase, pectinase, and xylanase, can limit the interaction between tannins and cell walls by modifying the cell wall structure. However, these enzymes did not significantly contribute to desorbing bound tannins.
Article
Biodiversity Conservation
Kathrin M. Bender, Mette M. Svenning, Yuntao Hu, Andreas Richter, Julia Schuckel, Bodil Jorgensen, Susanne Liebner, Alexander T. Tveit
Summary: The herbivory by barnacle geese in high-Arctic peatlands impacts vegetation cover, ecosystem productivity, and soil microbiota activity. Reduction in herbivory results in a different vegetation composition with increased input of polysaccharides to the soil, supporting a larger and more diverse microbiota community. Increased gene expression for polysaccharide degradation and elevated presence of predatory eukaryotes were observed in herbivory-excused areas.
Article
Plant Sciences
Florent Weiller, Julia Schueckel, William G. T. Willats, Azeddine Driouich, Melane A. Vivier, John P. Moore
Summary: This study examined the changes in grape cell wall composition during infection with Botrytis cinerea using comprehensive microarray polymer profiling and monosaccharide compositional analysis. The results showed degradation of pectin polymers during infection, with table grape cultivars exhibiting more severe symptoms compared to wine grape cultivars. Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility, with factors such as pectin methylesterification profiles and extensin reorganization potentially influencing resistance.
Article
Food Science & Technology
Gonzalo Garrido-Banuelos, Astrid Buica, Brock Kuhlman, Julia Schuckel, Anscha J. J. Zietsman, William G. T. Willats, John P. Moore, Wessel J. du Toit
Summary: The study explores the impact of different maceration times on the phenolic levels and evolution in young red wines, finding that longer maceration times do not always result in increased phenolic concentration, although complexity seems to be higher. Continuous depectination and possible solubilization of pectin observed during extended maceration may affect sensory perception of the wines, and maceration time also influences the polymeric fraction and sensory perception of the wines.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Rafael R. da Costa, Haofu Hu, Bo Pilgaard, Sabine M. E. Vreeburg, Julia Schuckel, Kristine S. K. Pedersen, Stjepan K. Kracun, Peter K. Busk, Jesper Harholt, Panagiotis Sapountzis, Lene Lange, Duur K. Aanen, Michael Poulsen
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Chemistry, Analytical
Julia Schuckel, Stjepan Kresimir Kracun, Thomas Frederik Lausen, William George Tycho Willats, Bodil Jorgensen
ANALYTICAL METHODS
(2017)
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Summary: Methylation plays important roles in various biological processes, and this study characterized the function of ThnM3 in regioselective modifications of 1,8-dihydroxynaphthalene.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
Summary: In this study, a highly sensitive and specific method using ultra performance liquid chromatography (UPLC) was developed to measure the enzymatic activity of APH4 in plants. The method exhibited good precision and accuracy, and was validated for use with samples from different sources.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Cuixia Zhou, Guangcheng Yang, Panpan Meng, Weishuai Qin, Yanyan Li, Zhenxian Lin, Wei Hui, Huitu Zhang, Fuping Lu
Summary: Bacillus licheniformis 2709 is an important industrial producer of alkaline protease (AprE), and this study identified the UP elements of the aprE gene and demonstrated the lack of significant effect of CggR, FruR, and YhcZ transcription factors on aprE expression. Enhanced aprE expression and enzyme activity were achieved by deleting a negative regulatory factor binding site in the genome. These findings provide valuable insights into the high-level production of proteins in other Bacillus species.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Summary: In this study, laccase was immobilized on modified magnetic nanoparticles, improving the enzyme's thermostability and efficiency for polymerization of phenols.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Handan Ustukarci, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
Summary: A biofilm layer called biofouling is formed over time by organic molecules and microorganisms on solid surfaces immersed in a liquid medium. This study focused on the antifouling performance of polyaniline (PANI) and investigated the immobilization of hydrolytic enzymes in PANI coatings. The results showed that the alpha-amylase-containing film exhibited the highest antifouling activity. Surface properties and biofilm composition were analyzed to support these findings.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo Jose Inocencio-Garcia, Carlos Ariel Cardona Alzate
Summary: This study evaluated the sustainability of biorefineries using orange peel as a raw material. By applying the concept of biorefineries and experimental data, it was found that steam distillation and polyphenolic compound extraction can improve the process performance, resulting in higher yields of different products. The integrated utilization of orange peel in biorefineries showed a high sustainability index.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)