期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 36, 期 2, 页码 724-731出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2013.06.012
关键词
Hypothermal stimulation; Cardiomyocytes; Extracellular regulated protein kinases 5; Bim
资金
- Shanghai Integrative Medicine Institute of Cardiovascular Disease, Shanghai, PR China
Mechanism of cold induced myocardial injury remained unclear. Our study investigated the role of ERK5/Bim pathway in hypothermal stimulation-induced apoptosis or damage of cardiomyocytes (CMs). Results showed that in CMs which under hypothermal stimulation, ERK5 siRNA promoted expression of Bim protein. Bim siRNA did not influence ERK5 expression but attenuated production of p-ERK5. ERK5 siRNA induced higher apoptosis rate; intracellular Ca2+ overload; ROS activity; Delta Psi m damage in hypothermia stimulated CMs, when compared with hypothermal stimulation solely treated group, while Bim siRNA effected oppositely and canceled pro-apoptotic effect of ERK5 siRNA. In conclusion, ERK5 knock down releases inhibition to Bim expression, induces. aggravated apoptosis in CMs under hypothermal stimulation, which related to higher intracellular Ca2+ overload, ROS activity, and more severe Delta Psi m damage. Results revealed regulative role of ERK5/Bim pathway in hypothermal stimulation-induced injure or apoptosis of cardiomyocytes. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据