Review
Biochemistry & Molecular Biology
Sian Thistlethwaite, Laura N. Jeffreys, Hazel M. Girvan, Kirsty J. McLean, Andrew W. Munro
Summary: BM3 is a catalytically self-sufficient flavocytochrome fusion protein that can be engineered to produce a variety of drug-like compounds. Through modification of key residues within the BM3 active site, its catalytic repertoire can be expanded to generate diverse metabolites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Peter D. Giang, Luke R. Churchman, Jeanette E. Stok, Stephen G. Bell, James J. De Voss
Summary: Rhodococcus globerulus, a bacterium, can utilize eucalypt oil as its only source of carbon and energy. Two cytochromes P450 from this organism, CYP176A1 and CYP108N12, initiate the biodegradation of monoterpenes in the oil. A specific [2Fe-2S] ferredoxin redox partner called cymredoxin has been isolated and characterized for CYP108N12, and it has been found to improve the catalytic ability of CYP108N12 and support the activity of other P450s.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Multidisciplinary Sciences
Tamara Heintze, Kathrin Klein, Ute Hofmann, Ulrich M. Zanger
Summary: The use of lentiviral transduction to deliver Cas9 and sgRNAs in HepaRG cells resulted in efficient knockdown of POR and revealed differential effects on drug-metabolizing CYP enzymes. Knockdown of POR also affected the expression of CYPs at mRNA and protein levels. The study highlights the importance of POR/NADPH- and CYB5/NADH-electron transport systems in influencing human drug metabolism, with implications for future research on hepatic functions.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Balazs Pogranyi, Tamara Mielke, Alba Diaz-Rodriguez, Jared Cartwright, William P. Unsworth, Gideon Grogan
Summary: The lyophilized preparation of unspecific peroxygenase variant from Agrocybe aegerita is an efficient catalyst for the oxygenation of a wide range of N-heterocyclic compounds. This method utilizes only H2O2 as the oxidant and can achieve high synthetic yields, regioselectivity and enantioselectivity. The versatility and scalability of UPOs highlight their potential as practical biocatalysts for oxygenation reactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Analytical
Li Mi, Ziteng Wang, Wenge Yang, Chaofeng Huang, Bin Zhou, Yonghong Hu, Songqin Liu
Summary: Cytochrome P450 (CYP450s) are a large superfamily of enzymes that catalyze versatile reactions introducing oxygen into molecules. However, the current biotechnologies are hindered by limited oxygen availability. This review summarizes methods for CYP450s immobilization, activity detection, and synthesis of complex products, and introduces a novel approach using a gas-solid-liquid interface to overcome oxygen limitation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Environmental Sciences
Dayse Carvalho da Silva Martins, Iasmin Tavares Resende, Bruno Jose Rocha da Silva
Summary: Pesticides are potentially toxic to target organisms and harmful to human health and the environment. Various degradation processes have been studied, but there are still gaps. This review focuses on the catalytic degradation of pesticides using porphyrins, which have been shown to be effective catalysts for degradation and a useful tool for studying degradation products. However, achieving complete mineralization of pesticides without intermediate products remains challenging.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Genetics & Heredity
Menna Al-Adl, Afaf El-Said, Ahmed EL-Sebaie, Sherif Refaat, Magdy M. Youssef
Summary: This study found that the CYP1A1*2A (T3801C, rs4646903) single nucleotide polymorphism (SNP) may be a risk factor for developing B-CLL, and the impact of this SNP on the disease progression and the clinical outcome.
Article
Engineering, Environmental
Huanni Zhang, Xiaoqing Wang, Runqian Song, Wen Ding, Fei Li, Li Ji
Summary: Metabolism, especially by CYP450 enzymes, plays a crucial role in mediating the toxification and detoxification of xenobiotics in humans. The metabolic pathways of emerging pollutants, which could potentially cause idiosyncratic toxicity, are often overlooked. This study focused on the sulfonamide antibiotics pollution in aqueous systems and investigated the metabolic processes of sulfamethoxazole (SMX), specifically the coupling and fragmenting initiated by amino H-abstraction catalyzed by human CYP450 enzymes. The findings emphasized the importance of using computations prior to experiments in environmental chemistry and toxicology.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Medicine, Research & Experimental
Mohammed G. Maslub, Mahasen A. Radwan, Nur Aizati Athirah Daud, Abubakar Sha'aban
Summary: This article reviews the association between genetic variations in CYP3A4/5 and the response to atorvastatin therapy globally, including its pharmacokinetics and the risk of adverse reactions. It also highlights the need for further studies in the Egyptian population.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Liping Chen, Ping Guo, Wenxue Li, Xinhang Jiang, Qun Zhao, Daochuan Li, Qing Wang, Yongmei Xiao, Xiumei Xing, Yaqin Pang, Michael Aschner, Lihua Zhang, Wen Chen
Summary: Protein phosphatase 2A (PP2A) is an enzyme that plays various roles in biological processes, but its specific actions in many critical pathways are unclear. Through a study on a mouse model, it was found that PP2A is involved in the regulation of liver metabolism, and specific PP2A enzymes contribute to the induction of metabolic enzymes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Alexander N. Grechkin, Natalia Lantsova, Bulat Khairutdinov, Yana Y. Toporkova
Summary: Genomic analysis of the cabbage clubroot pathogen revealed the presence of two expressed genes encoding P450 enzymes related to oxylipin biosynthesis. The recombinant protein from one of these genes successfully converted a specific substrate into novel oxylipins, providing insights into the conversion mechanism. This study sheds light on the role of the enzyme CYP50918A1 in controlling fatty acid hydroperoxide conversion and the formation of unique oxylipins in this pathogen.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Pharmacology & Pharmacy
Lenka Jourova, Stefan Satka, Veronika Frybortova, Iveta Zapletalova, Pavel Anzenbacher, Eva Anzenbacherova, Petra Petr Hermanova, Barbora Drabonova, Dagmar Srutkova, Hana Kozakova, Tomas Hudcovic
Summary: The development of inflammatory bowel disease (IBD) is closely related to changes in the gut microbiota. Butyrate, a gut microbiome-derived metabolite with anti-inflammatory effects, shows promise as a potential treatment option for IBD. This study aimed to investigate the protective effects of sodium butyrate (SB) administration on colonic inflammation and its effects on hepatic drug metabolism. The results revealed that butyrate pre-treatment can alleviate gut inflammation and improve the integrity of the colonic epithelium in mice with induced colitis. Additionally, butyrate was found to affect the expression and enzyme activity of certain cytochromes P450 (CYPs) in the liver. These findings highlight the importance of understanding the impact of gut inflammation and therapeutic interventions on drug metabolism in the context of IBD therapy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Lenka Jourova, Stefan Satka, Veronika Frybortova, Iveta Zapletalova, Pavel Anzenbacher, Eva Anzenbacherova, Petra Petr Hermanova, Barbora Drabonova, Dagmar Srutkova, Hana Kozakova, Tomas Hudcovic
Summary: This study demonstrates that pretreatment with butyrate can alleviate colonic inflammation and affect hepatic drug metabolism enzyme activity in mice, highlighting the importance of investigating the gut-liver axis in inflammatory bowel disease and microbiome-targeted interventions.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mariano Volonte, Lucila Traverso, Jose Manuel Latorre Estivalis, Francisca Cunha Almeida, Sheila Ons
Summary: This study performed a comparative analysis on the complement of xenobiotic detoxification gene superfamilies across five hemipteran species. The results showed that heteropterans have an expansion of detoxification enzymes compared to other species. Interestingly, the most polyphagous species analyzed here (H. halys) also has the largest repertoire of detoxification enzymes, which may be due to gene duplication events. These detoxification-related gene families play important roles in the detoxification of diet products and conferring tolerance or resistance to synthetic insecticides.
Article
Food Science & Technology
Xue-Yan Dai, Jia Lin, Shi-Yong Zhu, Jian-Ying Guo, Jia-Gen Cui, Jin-Long Li
Summary: The study revealed that lycopene (LYC) can alleviate the neurotoxicity caused by atrazine (ATR) by modulating nuclear receptors and CYP450. ATR induced oxidative stress and inflammatory damage, disrupted the balance of CYP450, but these effects were reversed by LYC pretreatment.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
