Article
Chemistry, Physical
Leo Sala, Hlib Lyshchuk, Jana Sachova, David Chvatil, Jaroslav Kocisek
Summary: DNA origami nanoframes were used to study the effect of nucleoside substitutions on radiation-induced DNA damage. The results showed that increased AT or GC content did not increase the number of DNA strand breaks. Different nucleoside analogues had different effects on DSB, indicating different radiosensitization mechanisms. Bystander effects were also observed when substitutions were introduced in the parallel DNA sequence. This new approach overcomes limitations of previous methods and enables detailed studies of poorly understood conformation or bystander effects in radiation-induced DNA damage.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Lucian Zastko, Anna Rackova, Petra Petrovicova, Matus Durdik, Jakub Misek, Eva Markova, Igor Belyaev
Summary: The dephosphorylation inhibitor calyculin A has been shown to have no effect on radiation-induced gamma H2AX foci at low doses, but decreases all types of DNA repair foci induced by 100 cGy irradiation. Calyculin A treatment induces apoptosis in UCBL, potentially leading to the failure to maintain radiation-induced gamma H2AX foci.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tamara Bakuradze, Peter Meiser, Jens Galan, Elke Richling
Summary: This study found that consuming an aronia juice-based food supplement significantly reduced H2O2-induced DNA strand breaks, but had a low overall effect on background and total DNA strand breaks. This suggests that antioxidant supplements may only have marginal preventive effects under healthy conditions.
Article
Biology
Sonia Spandole-Dinu, Ana-Maria Catrina, Oana Cristina Voinea, Alina Andone, Speranta Radu, Cerasela Haidoiu, Octavian Calborean, Radu Gabriel Hertzog, Diana Mihaela Popescu
Summary: This pilot study aimed to evaluate the potential radioprotective effect of Natural SOD (R) in Wistar rats exposed to ionizing radiation. The results showed a potential protective effect of Natural SOD (R) against radiation-induced DNA damage, particularly in the testes. Further investigations on the long-term effects of X-ray in animals administered Natural SOD (R) are needed for a better estimation of the radioprotective effect.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alexandr Zalessky, Yuriy Fedotov, Elizaveta Yashkina, Viktor Nadtochenko, Andreyan N. Osipov
Summary: The study found that different pulse power densities of femtosecond laser radiation can lead to the formation of XRCC1 and gamma H2AX protein foci, indicating that femtosecond laser radiation can be considered as a tool for local ionization of biological material.
Article
Biology
Galina A. Posypanova, Marya G. Ratushnyak, Yuliya P. Semochkina, Alexander N. Strepetov
Summary: The study aimed to investigate the effects of gamma-neutron radiation on mouse neural stem/progenitor cells. The research found that gamma-neutron radiation can cause cell death and proliferation, with a high relative biological efficiency. Special measures are needed to protect the neurogenic regions of the brain.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Review
Oncology
Davide Valente, Maria Pia Gentileschi, Antonino Guerrisi, Vicente Bruzzaniti, Aldo Morrone, Silvia Soddu, Alessandra Verdina
Summary: This article discusses the induction of gamma H2AX in individuals exposed to ionizing radiation and its applications in clinical studies. By analyzing the various conditions that induce H2AX phosphorylation and describing commonly used methods for gamma H2AX determination, the assessment of DSBs following IR exposure can be optimized.
Article
Biochemistry & Molecular Biology
Joelle Al-Choboq, Melanie L. Ferlazzo, Laurene Sonzogni, Adeline Granzotto, Laura El-Nachef, Mira Maalouf, Elise Berthel, Nicolas Foray
Summary: Usher syndrome is a rare genetic disease characterized by hearing loss, visual impairment, and vestibular dysfunctions. This study provides the first radiobiological characterization of cells from USH1 patients at both molecular and cellular levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Petr Heneberg
Summary: The proper course and reproducibility of diagnostic techniques depend on narrowly defined reaction conditions, including the reaction pH. Nevertheless, numerous assays are affected by an inaccurately defined reaction pH. This article focuses on the comet assay lysis buffer used for quantifying DNA breaks in eukaryotic cells. The pH of the lysis buffer used in the comet assay is often outside the useful range of the Tris buffer, causing imprecise and uncritical lysis conditions.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2023)
Article
Cell Biology
Fanghua Li, Emil Mladenov, Yanjie Sun, Aashish Soni, Martin Stuschke, Beate Timmermann, George Iliakis
Summary: Alt-EJ is an error-prone DNA double-strand break repair pathway that is active when primary repair pathways, c-NHEJ and HR, are defective or fail. It depends on DNA end-resection, initiated by the CtIP/MRE11-RAD50-NBS1 complex and extended by EXO1 or BLM/DNA2 complex. Alt-EJ is regulated by the cell cycle phase, with maximum activity in G(2)-phase and reduced activity in G(1)-phase and quiescent G(0)-phase cells. CtIP-dependent resection is identified as the key regulator of Alt-EJ in G(1)- and G(0)-phase cells, with low levels of CtIP allowing modest resection and Alt-EJ in G(1)-phase cells and undetectable CtIP and Alt-EJ in G(0)-phase cells.
Article
Mathematics
Dalal Yahya Alzahrani, Fuaada Mohd Siam, Farah A. Abdullah
Summary: This paper presents a mathematical approach using nonlinear differential equations to study immune cell populations. It successfully applies this approach to investigate the effects of radiation on DNA and cell populations. The method takes into account the memory and hereditary properties of the system, providing a good framework for describing these characteristics of immune cell populations.
Article
Chemistry, Multidisciplinary
Bowei Wang, Jianjun Dong, Fan Yang, Tuoyu Ju, Jiani Li, Junxi Wang, Ying Wang, M. James C. Crabbe, Yanling Tian, Zuobin Wang
Summary: This study used atomic force microscopy to detect the chromosomal structure changes in UVB-stimulated HaCaT cells. The results showed that higher UVB irradiation doses led to more cells with chromosome aberrations. Additionally, damage to both DNA and cells was observed under the corresponding irradiation doses, with the degree of DNA damage directly proportional to the irradiation dose. The mechanical properties of cells were also altered after UVB irradiation, indicating a series of chain reactions occurring in the cells.
Article
Public, Environmental & Occupational Health
Ghazi Alsbeih, Najla Al-Harbi, Sheikh Ismail, Michael Story
Summary: The study found significant differences in DNA double-strand break (DSB) repair fidelity among individuals with different radiosensitivity, with higher levels of misrepaired DSBs in radio-sensitive (RS) and ataxia-telangiectasia mutated (ATM) cells compared to normal control (NC). Misrepaired DSBs may serve as a potential marker for adverse reactions to radiotherapy, indicating the importance of DNA repair fidelity in determining individual radiosensitivity.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Public, Environmental & Occupational Health
Hongling Zhao, Minmin Qu, Yuchen Li, Ke Wen, Hua Xu, Man Song, Dafei Xie, Xingkun Ao, Yihao Gong, Li Sui, Hua Guan, Pingkun Zhou, Jianwei Xie
Summary: The study demonstrates the accuracy and potential utility of using mass spectrometry to quantify gamma-H2AX in human peripheral blood cells for assessing low-dose exposure to IR.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Plant Sciences
Beata P. Plitta-Michalak, Alice A. Ramos, Piotr Pupel, Marcin Michalak
Summary: The high-throughput comet assay is a sensitive tool for monitoring DNA integrity changes and assessing the viability status of plant germplasm.
Article
Biotechnology & Applied Microbiology
Rajeshkumar Anbazhagan, Ganesan Muthusamy, Rajakumari Krishnamoorthi, Swedha Kumaresan, Nagarajan Rajendra Prasad, Juin-Yih Lai, Jen-Ming Yang, Hsieh-Chih Tsai
Summary: In this study, FA and PTX co-loaded PAMAM dendrimers conjugated with RGD were prepared to overcome P-gp-mediated multidrug resistance. The RGD-functionalized dendrimers showed higher PTX delivery and enhanced anticancer potential in drug-resistant cells. This therapeutic strategy may induce apoptosis effectively in P-gp-overexpressing, multidrug-resistant cells.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Applied
Naziya Begum, N. Rajendra Prasad, G. Kanimozhi, B. Agilan
Summary: Pre-treatment with apigenin shows a protective effect against radiation-induced gastrointestinal damages in Swiss albino mice, improving intestinal architecture and modulating apoptotic signaling pathways.
NATURAL PRODUCT RESEARCH
(2022)
Review
Pharmacology & Pharmacy
G. Kanimozhi, B. Pradhapsingh, Charan Singh Pawar, Haseeb A. Khan, Salman H. Alrokayan, N. Rajendra Prasad
Summary: COVID-19 is a recent pandemic outbreak that requires fundamental research to understand its pathogenesis and potential drug targets.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Medicine, Research & Experimental
M. Ganesan, G. Kanimozhi, B. Pradhapsingh, Haseeb A. Khan, Abdullah S. Alhomida, Aishah Ekhzaimy, G. R. Brindha, N. Rajendra Prasad
Summary: The review discusses the role of natural phytochemicals in modulating ABCB1 gene expression through various signal transduction pathways in MDR cancer cells, suggesting an alternative approach to reversing MDR by downregulating P-glycoprotein expression in tumor tissues.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Physiology
Sergey V. Popov, Alexander V. Mukhomedzyanov, Sergey Y. Tsibulnikov, Igor Khaliulin, Peter R. Oeltgen, N. Rajendra Prasad, Leonid N. Maslov
Summary: This study demonstrated that the activation of opioid kappa(2) receptor does not affect cardiac tolerance to reperfusion, while peripheral opioid kappa(1) receptor stimulation can prevent reperfusion cardiac injury. Additionally, administration of ICI 199,441 at reperfusion showed a reduction in infarct size.
PHYSIOLOGICAL RESEARCH
(2021)
Review
Cardiac & Cardiovascular Systems
Leonid N. Maslov, Sergey V. Popov, Alexandr V. Mukhomedzyanov, Natalia V. Naryzhnaya, Nikita S. Voronkov, Vyacheslav V. Ryabov, Alla A. Boshchenko, Igor Khaliulin, N. Rajendra Prasad, Feng Fu, Jian-Ming Pei, Sergey V. Logvinov, Peter R. Oeltgen
Summary: Ca2+ overload and increased reactive oxygen species production play a significant role in reperfusion injury of cardiomyocytes. The NLRP3 inflammasome and increased adrenergic system activity during coronary perfusion restoration worsen the damage to the heart.
CURRENT CARDIOLOGY REVIEWS
(2022)
Article
Biochemical Research Methods
M. Sudharsan, N. Rajendra Prasad, Anindita Chakraborty, Saravanan Rajendrasozhan
Summary: This study emphasizes the significance of cellular redox system in preserving the proteome of the radioresistant Deinococcus radiodurans. The inhibition of thioredoxin reductase redox system was found to affect the expression of essential cellular proteins for survival. These findings contribute to a better understanding of the survival mechanism of Deinococcus radiodurans and the potential application of its cellular content in handling redox imbalances during cancer radiotherapy.
JOURNAL OF PROTEOMICS
(2022)
Review
Biotechnology & Applied Microbiology
Priya Yadav, Suresh V. Ambudkar, N. Rajendra Prasad
Summary: Cancer often develops multidrug resistance (MDR) when cancer cells become resistant to numerous chemotherapeutic agents. Researchers have designed small molecule inhibitors and derivatives of natural compounds to overcome MDR. Nanomedicine approaches provide an efficient drug delivery platform to improve therapeutic effectiveness. This review critically discusses the major factors contributing to MDR and reviews recent nanotechnology-based approaches to overcome clinical MDR.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
M. Sudharsan, N. Rajendra Prasad, Rajendrasozhan Saravanan
Summary: Bacteria have evolved various cellular mechanisms to survive in unfavorable environmental conditions. Antioxidants and stress regulators play a significant role in their survival. Transcriptional regulators sense and respond to oxidative stress, promoting the expression of antioxidant enzymes. The redox system also provides reducing equivalents for cellular repair mechanisms. Targeting these systems could alter cellular redox homeostasis. This review presents the mechanisms of different redox systems that favor bacterial survival in extreme environmental oxidative stress conditions.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pradhapsingh Bharathiraja, David S. P. Cardoso, N. Rajendra Prasad, Silva Mulhovo, Deepa S. Lakra, Maria-Jose U. Ferreira
Summary: This study found that derivatives of N-alkylated monoterpene indole alkaloids, particularly N-(para-bromobenzyl) (NBBT), can reverse P-gp-mediated multidrug resistance in cancer cells. NBBT exhibited the highest P-gp inhibitory activity and reduced P-gp overexpression by inhibiting the nuclear translocation of the nuclear factor kappa B p-50 subunit. Additionally, NBBT showed synergistic effects with doxorubicin, decreasing the cellular migration and increasing apoptotic incidence in drug-resistant cells.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Chemistry, Applied
Deepa Swati Lakra, B. Pradhapsingh, N. Deepika, T. Dhanalakshmi, G. Kanimozhi, Rajendra N. Prasad
Summary: This study evaluated the chemosensitizing property of Andro in P-gp overexpressing multidrug-resistant (MDR) colchicine-selected KBCh(R) 8-5 cells. Molecular docking studies showed Andro exhibits higher binding interaction with P-gp than other studied transporters. Furthermore, Andro downregulates P-gp overexpression via NF-kappa B signaling in MDR cell lines and enhances the therapeutic effect of PTX in drug-resistant cells.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Deepika Natarajan, N. Rajendra Prasad, M. Sudharsan, Pradhapsingh Bharathiraja, Deepa Swati Lakra
Summary: In this study, auranofin, an FDA-approved drug, has been repurposed for its anticancer activity. The results demonstrate that auranofin enhances the cytotoxicity of paclitaxel in breast cancer cell lines. Auranofin alters the intracellular redox balance in breast cancer cells, leading to paclitaxel-induced cell cycle arrest and subsequent apoptosis. Therefore, auranofin could be considered as a chemosensitizing agent during cancer chemotherapy.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Biochemistry & Molecular Biology
Rajendran Indirapriyadarshini, Thangaiyan Radhiga, Govindasamy Kanimozhi, N. Rajendra Prasad
Summary: This study demonstrates that andrographolide (ADP) can protect human dermal fibroblasts (HDFa) from oxidative stress and apoptosis induced by UVB radiation.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Pharmacology & Pharmacy
Pradhapsingh Bharathiraja, Priya Yadav, Andaleeb Sajid, Suresh V. Ambudkar, N. Rajendra Prasad
Summary: ABCB1, ABCG2, and ABCC1 are major players in multidrug resistance (MDR) caused by drug efflux, but their expression can be downregulated by natural medicinal compounds through modulation of transcription factors and associated signaling pathways.
DRUG RESISTANCE UPDATES
(2023)
Article
Cardiac & Cardiovascular Systems
Leonid N. Maslov, Sergey Popov, Alexandr Mukhomedzyanov, Ivan A. Derkachev, Vyacheslav V. Ryabov, Alla A. Boshchenko, N. Rajendra Prasad, Galina Z. Sufianova, Maria S. Khlestkina, Ilgiz Gareev
Summary: This article discusses pharmacological approaches to limit ischemic and reperfusion injuries of the heart, with a focus on the effects of P2Y12 receptor antagonists. Experimental and clinical data both support the cardioprotective effects of ticagrelor, suggesting that sphingosine-1-phosphate may mediate this effect. The role of adenosine in the cardioprotective effect of ticagrelor is also discussed.
KOREAN CIRCULATION JOURNAL
(2022)
