Responses of Glutathione-Related Antioxidant Defense System in Serum of Nile Tilapia (Oreochromis niloticus) Exposed to Sublethal Concentration of Methomyl and Recovery Pattern

Tilapia were exposed to sublethal concentrations of 0, 0.2, 2, 20, or 200 g/L for 30 days, and then transferred to methomyl-free water for 18 days. GST, GPx, GR, GSH, and GSSG in tilapia serum were examined at 0, 6, 12, 18, 24, and 30 days after methomyl exposure and at 18 days after transferring to methomyl-free water. There were no significant changes in antioxidants activities and contents in serum of tilapia exposed to 0.2 g/L. Significant increases in GST, GR, GPx, and GSSG accompanied by a decrease in GSH were observed following methomyl exposure to 2, 20, or 200 g/L, suggesting the presence of oxidative stress. Thus, it would appear the 0.2 g/L methomyl might be considered the no observed adverse effect level. Recovery data showed that the effects produced by lower concentration of 20 g/L were reversible but not at the higher 200 g/L concentration. (c) 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 483-489, 2015.