Article
Engineering, Environmental
Marwa M. El-Dalatony, Bikram Basak, Mayur B. Kurade, Hyun-Seog Roh, Min Jang, Byong-Hun Jeon
Summary: This study demonstrates the effective production of biohydrogen (bioH(2)) and ABE through ABE fermentation using sonication pretreated Chlamydomonas mexicana biomass as a cost-effective substrate. Sonication enhances the recovery and bioaccessibility of carbohydrates and proteins, resulting in higher yields of bioH(2) and volatile fatty acids. The study shows the efficient conversion of soluble carbohydrates and proteins into bioH(2) and ABE, which can be used for high-energy biofuel production with minimal waste generation.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Petra Patakova, Barbora Branska, Maryna Vasylkivska, Katerina Jureckova, Jana Musilova, Ivo Provaznik, Karel Sedlar
Summary: Solventogenic clostridia, although not a strictly defined group within the genus Clostridium, share common features such as being anaerobic, non-pathogenic, non-toxinogenic, and endospore forming bacteria. Their main metabolite is typically 1-butanol, but they can also produce other metabolites depending on species and culture conditions. Despite being used for industrial production in the past, they were replaced by more efficient chemical production methods. Transcriptome analysis is a valuable tool for gaining deeper insights into clostridial metabolism and life.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Wei-Lun Chang, Wenjie Hou, Mengmeng Xu, Shang-Tian Yang
Summary: This study achieved continuous production of butanol from glucose and butyrate by immobilizing cells in a single-pass fibrous-bed bioreactor. The high density of immobilized cells improved the tolerance to butanol and butyric acid, resulting in significantly increased productivity.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Celine Foulquier, Antoine Riviere, Mathieu Heulot, Suzanna Dos Reis, Caroline Perdu, Laurence Girbal, Mailys Pinault, Simon Dusseaux, Minyeong Yoo, Philippe Soucaille, Isabelle Meynial-Salles
Summary: The authors identified the previously unknown encoding genes for important enzymes involved in redox balancing in n-butanol production by Clostridium acetobutylicum, and increased n-butanol production through enzyme optimization. This discovery is significant for the development of a homobutanologenic C. acetobutylicum strain.
NATURE COMMUNICATIONS
(2022)
Article
Energy & Fuels
F. Raganati, A. Procentese, G. Olivieri, M. E. Russo, P. Salatino, A. Marzocchella
Summary: This study demonstrates the intensive production of butanol using immobilized cells of Clostridium acetobutylicum in a novel continuous fermentation system. By implementing a stagewise simulated moving bed design and integrating the reaction and the product recovery sections, the system achieves high productivity and concentration of butanol.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2022)
Article
Biotechnology & Applied Microbiology
Karlo Guerrero, Roberto Gallardo, Ernesto Gonzalez, Fabian Veliz, Raul Conejeros, Juan Carlos Gentina, German Aroca
Summary: By adding butyrate and neutral red to the culture medium, and using electro-fermentation with an external electron source, the production of butanol can be increased. However, the presence of butyrate and the absence of neutral red in electro-fermentation are detrimental to the microorganism.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Energy & Fuels
Zhiyou Zhou, Yujie Jing, Saijin Wei, Qinghua Zhang, Shuaiying Peng, Xuejiao An, Hanguang Li
Summary: In this study, an exogenous regulatory strategy named oxidation-reduction potential (ORP) regulation was used to enhance butanol production. The findings showed that controlling the ORP value at -400 mV resulted in the highest butanol production, with increased yield and productivity. The study also investigated the physiological mechanisms of ORP regulation on butanol synthesis.
Article
Biochemistry & Molecular Biology
Youduo Wu, Zhenzhong Wang, Xiao Ma, Chuang Xue
Summary: By optimizing high-temperature simultaneous saccharification and fermentation (SSF) technology and using genetically engineered thermotolerant Clostridium acetobutylicum, the production and productivity of lignocellulosic butanol were successfully increased, demonstrating its potential in improving butanol production efficiency.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
M. Capilla, P. San-Valero, M. Izquierdo, J. M. Penya-roja, C. Gabaldon
Summary: This study evaluated the influence of initial glucose concentrations and pH control strategies on biobutanol production and glucose consumption. The results showed that different pH control conditions had a significant impact on butanol production under different initial glucose concentrations.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Agricultural Engineering
Ibham Veza, Mohd Farid Muhamad Said, Zulkarnain Abdul Latiff
Summary: This review article discusses the history of the butanol industry and recent advances in butanol production by ABE fermentation, highlighting novel approaches such as searching for abundant and inexpensive substrates and optimizing processes. The challenges of biobutanol production, including low yield and end-product toxicity, as well as potential research routes, are summarized, along with the future research direction of butanol production by ABE fermentation.
BIOMASS & BIOENERGY
(2021)
Article
Chemistry, Multidisciplinary
Guangqing Du, Chao Zhu, Mengmeng Xu, Lan Wang, Shang-Tian Yang, Chuang Xue
Summary: This study identified four orphan histidine kinases (HKs) that control sporulation and solventogenesis in Clostridium acetobutylicum. Single knockout of cac3319 halted sporulation at the initiation stage but increased butanol tolerance and production, while cac0437 knockout caused premature sporulation and inhibited the transition to solventogenesis, resulting in low butanol production.
Article
Biochemistry & Molecular Biology
Guangqing Du, Youduo Wu, Wei Kang, Yongping Xu, Shuying Li, Chuang Xue
Summary: In this study, the strain Clostridium acetobutylicum ATCC 55025 and its mutant HKKO were engineered to improve butanol production. Knocking out the acetoacetate decarboxylase gene adc decreased acetone production and increased the butanol/ABE ratio. Overexpression of the acetaldehyde/alcohol dehydrogenase gene adhE2 further increased butanol production.
PROCESS BIOCHEMISTRY
(2022)
Article
Agricultural Engineering
J. Iyyappan, B. Bharathiraja, Sunita Varjani, R. PraveenKumar, S. Muthu Kumar
Summary: In this study, microbial reduction of black strap molasses was performed using Clostridium acetobutylicum MTCC 11,274 to produce biobutanol. The significant parameters affecting butanol production were identified and optimal fermentation conditions were determined. The results showed that waste black strap molasses could be a promising substrate for the production of next generation biofuel.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Guangqing Du, Jian Che, Youduo Wu, Zhenzhong Wang, Zhiyang Jiang, Feng Ji, Chuang Xue
Summary: In this study, the disruption of hydA gene in Clostridium acetobutylicum led to increased butanol production and decreased acetone formation. Exogenous supplementation of methyl viologen further improved the butanol yield and ratio. The engineered strain showed potential for producing biobutanol using lignocellulosic biomass.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Energy & Fuels
Sarita Sae-hun, Piyawat Chinwatpaiboon, Akarin Boonsombuti, Ancharida Savarajara, Apanee Luengnaruemitchai
Summary: This study applied immobilization technique to improve cell culture in ABE fermentation and selected cassava rhizome charcoal, bamboo charcoal, and coconut shell activated carbon as carriers. The results showed that the immobilized cells on bamboo charcoal produced higher butanol than free cells, and cassava rhizome charcoal exhibited the highest cell efficiency after sequential reuse cycles.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
