Article
Multidisciplinary Sciences
Saleem Farooq, Ruqeya Nazir, Shabir Ahmad Ganai, Bashir Ahmad Ganai
Summary: This study isolated a cold-active protease Apr-BO1 from psychrotrophic bacteria, showing optimal activity at 20 degrees C and pH 9.0, making it a potential detergent additive.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Chemical
Ailton Cesar Lemes, Gabrielle Victoria Gauterio, Cezar Augusto da Rosa, Adriano Brandelli, Susana Juliano Kalil
Summary: This study purified and characterized a keratinolytic protease produced by Bacillus sp. P45 through the bioconversion of feather meal. The purification process involved the use of aqueous two-phase systems (ATPS) and diafiltration (DF) to obtain a highly purified enzyme. The purified protease was characterized in terms of protein profile, optimum temperature and pH, thermal deactivation, and performance in the presence of salts and organic solvents. The results provide valuable information for future studies on enzyme purification and the application of protease in various industrial processes.
Article
Microbiology
Kadriye Inan Bektas, Aleyna Nalcaoglu, Esma Ceylan, Disat Nigar Colak, Pinar Caglar, Sevda Agirman, Nur Sena Sivri, Sueda Gunes, Alanur Kaya, Sabriye Canakci, Ali Osman Belduz
Summary: Detergent-compatible enzymes, especially microbial enzymes, are preferred in the detergent industry due to their stability, affordability, and availability. In this study, soil samples contaminated with household waste were collected and 55 bacterial isolates were obtained, with 25 isolates showing positive results in enzyme screening. The enzymes produced by these isolates, including amylase, lipase, cellulase, and protease, hold great promise for the detergent industry based on their characteristics and potential applications.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Wei Zhang, Jintao Wu, Jing Xiao, Mingyao Zhu, Haichuan Yang
Summary: Protease is an important component of detergents, and its combination with additives can enhance the washing effect. This experiment used various methods to evaluate the whiteness and stain residues on fabric samples before and after washing. A protease detergent formula with superior decontamination and anti-deposition effects was selected. The combination of alkaline protease, keratinase, and trypsin showed cost-effective stain removal. In addition, the composite protease exhibited better overall decontamination than commercial detergents when used with suitable surfactants, anti-redeposition agents, and water-softening agents.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Xiao Wang, Xing Qin, Lige Tong, Jie Zheng, Tao Dong, Xiaolu Wang, Yuan Wang, Huoqing Huang, Bin Yao, Honglian Zhang, Huiying Luo
Summary: A serine protease gene, Fgapt4, was cloned and mutated to obtain the best mutant FgAPT4- M3 with higher catalytic activity and detergent performance. It showed great potential in the detergent industry.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Leonhard H. Urner, Ehsan Mohammadifar, Kai Ludwig, Denis Shutin, Francesco Fiorentino, Idlir Liko, Fernando G. Almeida, Daniel Kutifa, Rainer Haag, Carol Robinson
Summary: dPGS-PCL serves as a novel purification agent for membrane protein research, allowing for the study and purification of membrane proteins. Results demonstrate that protein yields and delipidation outcomes are influenced by the properties of membrane mimetics and membranes.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Microbiology
Marwa Elhamdi, Sofiane Ghorbel, Noomen Hmidet
Summary: A novel alkalophilic bacterium, Bacillus swezeyi B2, with proteolytic activity of 1400 U/ml was isolated from contaminated soil. The crude enzyme likely contained a single extracellular protease, which was highly stable and active under alkaline and high temperature conditions. It remained stable in the presence of surfactants, oxidizing agents, and commercial detergents, and showed excellent stain removal efficacy.
CURRENT MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Veronica Zampieri, Cecile Hilpert, Melanie Garnier, Yannick Gestin, Sebastien Delolme, Juliette Martin, Pierre Falson, Guillaume Launay, Vincent Chaptal
Summary: The Det.Belt server allows researchers to visually represent membrane proteins wrapped by detergents in 3D, aiding in a better understanding of the interaction between the sample and its environment.
Review
Biotechnology & Applied Microbiology
Francois N. Niyonzima
Summary: Detergent enzymes are widely used in detergents to replace toxic substances, reduce energy consumption, and preserve the shape and color of washed garments. Fungi are a major source of these enzymes, and genetic engineering has enabled high production of detergent-compatible enzymes.
FOLIA MICROBIOLOGICA
(2021)
Article
Multidisciplinary Sciences
Isha Sharma, Kumar Pranaw, Hemant Soni, Hemant Kumar Rawat, Naveen Kango
Summary: This study evaluated the production of keratinase from a newly isolated B. velezensis NCIM 5802 and improved the production and feather degradation through optimization. The study also explored the biochemical properties of the keratinase and the keratin degradation mechanism through various analyses, confirming its effectiveness and multiple forms.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Applied
Yunbing Tan, Donpon Wannasin, David Julian McClements
Summary: This study investigated the emulsifying properties of two potato protein fractions, PP-200 and PP-300. Both fractions successfully formed emulsions with small droplets under suitable conditions. However, they exhibited different stability to changes in pH and the presence of salt.
FOOD HYDROCOLLOIDS
(2023)
Review
Chemistry, Multidisciplinary
Gabrielle Foran, Nina Verdier, David Lepage, Arnaud Prebe, David Ayme-Perrot, Mickael Dolle
Summary: Epoxide-based solid polymer electrolytes are a promising option for all-solid-state batteries, offering advantages such as flexibility, tunability, and ease of processing. Some epoxide-containing polymers can be processed without solvents, allowing for direct dissolution of lithium salts and mild reaction conditions.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemical Research Methods
Asiye Hemsinli, Sumeyra Gurkok
Summary: This study isolated and characterized a novel strain that produces alkaline protease from Lake Van, and demonstrated its potential application in the detergent industry. The enzyme showed compatibility with commercial detergents and effectively removed blood and chocolate stains from fabrics.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemical Research Methods
Sumeyra Gurkok, Murat Ozdal
Summary: A lipase-producing bacterium Aeromonas caviae LipT51 isolated from Erzurum was identified and characterized, showing stable and effective performance in laundry detergent. This alkaline and thermostable lipase has the potential to enhance washing performance by improving oil-stain removal.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Multidisciplinary Sciences
Ghadah A. AlShaikh-Mubarak, Essam Kotb, Amira H. Alabdalall, Munirah F. Aldayel
Summary: Ninety-one elastase-producing bacterial isolates were recovered from different localities of the Eastern Province of Saudi Arabia. Elastase from the best isolate Priestia megaterium gasm32, from luncheon samples was purified to electrophoretic homogeneity using DEAE-Sepharose CL-6B and Sephadex G-100 chromatographic techniques. The purified elastase showed high antibacterial activity and potential for treating damaged skin fibers with the inhibition of contaminating bacteria.
Article
Environmental Sciences
Jasneet Grewal, Rameshwar Tiwari, S. K. Khare
WASTE AND BIOMASS VALORIZATION
(2020)
Article
Environmental Sciences
Ayesha Sadaf, Razi Ahmad, Achraf Ghorbal, Walid Elfalleh, Sunil Kumar Khare
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Chemistry, Physical
Pratibha Yadav, Sunil K. Khare, Satyawati Sharma
INTERNATIONAL JOURNAL OF CHEMICAL KINETICS
(2019)
Article
Biotechnology & Applied Microbiology
Ayesha Sadaf, Rajeshwari Sinha, Sunil K. Khare
Article
Biochemistry & Molecular Biology
Sam Joy, Sunil K. Khare, Shashi Sharma
PROCESS BIOCHEMISTRY
(2020)
Article
Environmental Sciences
Amrik Bhattacharya, Ayesha Sadaf, Swati Dubey, Rajesh P. Singh, Sunil Kumar Khare
Summary: In this study, bacterial nanocellulose (BNC) was successfully produced using Komagataeibacter xylinus strain SGP8, and characterized using SEM, XRD, and TGA. The BNC exhibited nanofibrillar structure and high crystallinity index, showing excellent adsorption performance for Cd (II).
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Microbiology
Ram Karan, Sam Mathew, Reyhan Muhammad, Didier B. Bautista, Malvina Vogler, Jorg Eppinger, Romina Oliva, Luigi Cavallo, Stefan T. Arold, Magnus Rueping
Article
Food Science & Technology
Tanya Nagpal, Shahenvaz Alam, Sunil K. Khare, Santosh Satya, Shivani Chaturvedi, Jatindra K. Sahu
Summary: In the study, it was found that Psidium guajava leaves extract inhibited lipid oxidation and decreased the formation of acrylamide in deep-fried food, providing oxidative stability.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Review
Food Science & Technology
Tanya Nagpal, Jatindra K. Sahu, Sunil K. Khare, Khalid Bashir, Kulsum Jan
Summary: Trans fats are desired by the edible oil industry for their functionality, but they have adverse effects on human health and nutrition in food. Global regulatory actions have been taken to eliminate trans fats from the food supply, with the World Health Organization leading a REPLACE action plan to achieve this by 2023. Various technologies have been developed to reduce trans fats in food, with interesterification and oleogelation emerging as promising methods.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Shahenvaz Alam, Shine Augustine, Tarun Narayan, John H. T. Luong, Bansi Dhar Malhotra, Sunil K. Khare
Summary: The study demonstrated the rapid and simple electroanalysis of acrylamide using a gold electrode modified with gold nanoparticles and dithiothreitol, which exhibited enhanced sensitivity and selectivity. Differential pulse voltammetry was used to detect ACR with low limits of detection and quantitation, and a wide linear range. The sensor's estimation of ACR levels in potato chips and coffee samples was consistent with high-performance liquid chromatography results.
Article
Biotechnology & Applied Microbiology
Amrik Bhattacharya, S. K. Khare
Summary: This study investigates the remediation potential of Bacillus cereus EMB20's β-lactamase preparation for the removal of Ampicillin (Amp) and other beta-lactam antibiotics. Results show that the enzyme is effective in completely remediating Amp at different concentrations within an hour, and its activity is not limited to Amp but also extends to other antibiotics.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Polymer Science
Sunny Malik, Ankita Maurya, Sunil Kumar Khare, Kinshuk Raj Srivastava
Summary: Plastic materials are difficult to degrade in the open environment, leading to the infiltration of plastic wastes into natural resources. The current disposal methods are inefficient, and recycling is challenging due to the mixed composition of plastic waste. This research analyzed previously published reports on the biodegradation of different plastic types and found that microbial degradation patterns follow the chemical classification of plastic types. The study suggests potential genera for the bioremediation of mixed plastic waste.
Article
Materials Science, Biomaterials
Indranil Mondal, Syeda Warisul Fatima, Swati Priya, Sagar Sengupta, Sunil Kumar Khare, Ritu Kulshreshtha
Summary: A novel nanostructure called TGNFs has been developed for efficient delivery of anti-miR-210 to glioblastoma cells, resulting in decreased proliferation and migration and increased apoptosis. This method shows potential as a delivery agent in miRNA inhibition therapy.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Nikky Goel, Moumita Ghosh, Deepti Jain, Rajeshwari Sinha, Sunil Kumar Khare
Summary: Biofilm-associated disorders, caused by Pseudomonas aeruginosa, are difficult to treat due to their resistance to the host's immune system and stress factors. In this study, a rare halophilic actinobacteria, Nocardiopsis lucentensis EMB25, was isolated and its secondary metabolites were found to inhibit and eradicate P. aeruginosa biofilm. The secondary metabolites target the quorum sensing pathway and bind to LasR and RhlR. The downregulation of various QS system genes further confirmed the action of secondary metabolites on LasR and RhlR, making actinobacteria a potential source for novel bioactives against biofilm-related infections.
RSC MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Syeda Warisul Fatima, Khalid Imtiyaz, Mohammad M. Alam Rizvi, Sunil K. Khare
Summary: The study examined the in vitro effects of microbial transglutaminase nanoflowers on breast cancer cells, revealing anti-cancer properties and significantly improved therapeutic efficacy on MCF-7 cells. The nanoflowers demonstrated cytotoxic effects and potential for cancer treatment.