Article
Environmental Sciences
Wang Lin, Huimin Luo, Jingyi Wu, Xiangli Liu, Beibei Cao, Yuqing Liu, Pinhong Yang, Jifeng Yang
Summary: The coexistence of eutrophication and plastic pollution in the aquatic environment is a global water pollution problem. The presence of polystyrene microplastics (PSMPs) increases the accumulation of microcystin-LR (MC-LR) in zebrafish gonads, exacerbating reproductive damage and endocrine disruption. These results show that PSMPs can serve as carriers to increase the bioaccumulation of MC-LR and aggravate reproductive damage and endocrine disruption.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Endocrinology & Metabolism
Peilei Shen, Shuqin Ji, Xulin Li, Qingning Yang, Bingxian Xu, Chris Kong Chu Wong, Liping Wang, Lei Li
Summary: Male reproductive function is essential for species continuation and is regulated by various stressors, including inflammation. In this study, acute systemic inflammation induced by lipopolysaccharide (LPS) injection compromised male fecundity, leading to decreased testosterone level, damaged spermatogenesis, and downregulation of testicular genes involved in steroidogenesis and blood-testis barrier. The testis was found to be more sensitive to LPS-induced acute stress compared to the adrenal gland. LPS treatment also caused disturbances in the hypothalamic-pituitary-gonad axis function, with higher activation of the medial preoptic area (mPOA) in the hypothalamus and elevated levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Brain mapping analysis revealed broad activation of various brain nuclei, accompanied by abnormal animal behavior. These findings suggest that LPS-induced inflammation not only causes local testicular damage but also disrupts the brain-testis axis at the systemic level.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Yamin Raza, Emily Mertens, Lauren Zink, Zhe Lu, Jon A. Doering, Steve Wiseman
Summary: This study investigated the effects of benzotriazole ultraviolet stabilizers (BUVSs) on the reproductive success of zebrafish. The results showed that although there was no significant effect on fecundity, UV-P exposure had a significant impact on fertilization success. In addition, exposed male fish exhibited decreased synthesis of sex steroids, decreased transcript abundances of genes involved in spermatogenesis, and decreased sperm quantity, suggesting that embryonic exposure to UV-P may impair the reproductive function of male fish.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ilaria Gentile, Valeria Vezzoli, Sara Martone, Maria Grazia Totaro, Marco Bonomi, Luca Persani, Federica Marelli
Summary: In this study, we investigated the effects of BaP on the HPG axis in zebrafish embryos. The results showed that BaP compromised the neuronal architecture of the GnRH3 network and upregulated antioxidant activity, oxidative DNA damage, and apoptosis pathways. These findings suggest that short-term exposure to BaP can adversely affect GnRH3 development in zebrafish embryos through a neurotoxic mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Asma' 'Afifah Shamhari, Zariyantey Abd Hamid, Siti Balkis Budin, Nurul Jehan Shamsudin, Izatus Shima Taib
Summary: BPA and its analogues have negative effects on the physiological functions of hormones in the male reproductive system, causing alterations in the HPC axis, steroidogenesis, and spermatogenesis.
Review
Food Science & Technology
Antonio Casas-Rodriguez, Ana M. Camean, Angeles Jos
Summary: Microcystins and cylindrospermopsin can induce toxic effects in various systems and organs, including potential endocrine disruption. However, research on their endocrine disruption activity is limited and existing results are contradictory. Further studies are needed to assess the risks and understand the dose-response relationships.
Article
Food Science & Technology
Chenyan Hu, Yachen Bai, Jing Li, Baili Sun, Lianguo Chen
Summary: Methylparaben (MeP), commonly used in daily products as a preservative, has become a concerning aquatic pollutant. This study exposed adult zebrafish to different concentrations of MeP to investigate its reproductive toxicity. The results showed that MeP exposure affected gonad development, disrupted hormone balance, and led to developmental deficits in offspring. This study provides insight into the antiestrogenic activity and reproductive toxicity of MeP.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Marine & Freshwater Biology
Tianyu Wang, Shanshan Wu, Jianjie Chen, Lijuan Li, Jinling Cao
Summary: This study investigated the alleviating effects of sesamin on thyroid endocrine disruption caused by fluoride in zebrafish. Sesamin significantly improved growth performance and reduced mortality rate in adults, while also improving hatching rate and body length and alleviating spinal curvature and cyst formation in embryos and larvae. It also alleviated damage to thyroid tissues, reduced oxidative stress, improved thyroid hormone imbalance, and reversed the expression of endocrine-related genes.
AQUATIC TOXICOLOGY
(2023)
Article
Environmental Sciences
Ming Jia, Miaomiao Teng, Sinuo Tian, Jin Yan, Zhiyuan Meng, Sen Yan, Ruisheng Li, Zhiqiang Zhou, Wentao Zhu
Summary: The study found that long-term low-dose exposure to PEN did not significantly affect the growth and spawning of zebrafish, but led to significant changes in important hormones and related gene expressions in zebrafish.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Reproductive Biology
Yuna Lee, Saniya Rattan, Radwa Barakat, Zane Inman, Kathy M. De La Torre, Daryl D. Meling, Marcia H. Monaco, Joseph M. Irudayaraj, Isaac K. Cann, Chemyong J. Ko, Sharon M. Donovan, Jodi A. Flaws, Genoa R. Warner
Summary: DEHP, a commonly used chemical, is found in many consumer products. Research has shown that neonatal exposure to DEHP in pigs leads to sex-specific disruption of the reproductive system, affecting both ovaries and testes.
REPRODUCTIVE TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Maha A. Salem, Raed S. Ismail, Hala F. Zaki, Hossam M. M. Arafa, Aiman S. N. El-Khatib
Summary: In this study, we investigated the potential of L-carnitine (LC) to mitigate the reproductive toxic effects of carbendazim (CBZ) in male rats, and explored the molecular mechanisms involved in modifying spermatogenic and steroidogenic disruptions caused by the endocrine disruptor. LC supplementation improved sperm quality, testicular histological alterations, endocrine disturbances, and modulation of gene expressions and proteins, primarily through its endocrine-preserving, antioxidant and anti-inflammatory properties.
Article
Biochemistry & Molecular Biology
Xuan Liu, Xianyi Xie, Hongling Liu
Summary: Exposure to environmental estrogen during the critical gonadal differentiation period not only affects the development of zebrafish, but also influences the population development, leading to a biased sex ratio in the fish population.
Article
Environmental Sciences
Sailo Lalrinzuali, Maurya Khushboo, Roy Dinata, Baishya Bhanushree, Nisekhoto Nisa, Rema Momin Bidanchi, Saeed-Ahmed Laskar, Bose Manikandan, Giri Abinash, Buragohain Pori, Vikas Kumar Roy, Guruswami Gurusubramanian
Summary: Long-term consumption of fermented pork fat (FPF) food with excessive calories, high fat content, and high FAME levels induces oxidative stress, inflammation, and apoptosis, resulting in metabolic syndrome and hampering male reproductive system and functions.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Marine & Freshwater Biology
Naima Hamid, Muhammad Junaid, Rakia Manzoor, Jin-Jing Duan, Ming Lv, Nan Xu, De-Sheng Pei
Summary: Chronic exposure to PPCP mixtures at environmentally relevant concentrations resulted in high accumulation in liver and gonadal tissues of adult zebrafish, leading to perturbed genetic responses and disruption of the HPG axis pathway. In silico molecular docking revealed specific amino acid residues of PPCPs binding with estrogen receptors, confirming their xenoestrogenic behavior. Strict environmental regulations for the disposal of PPCPs are crucial to protect ecological and public health.
AQUATIC TOXICOLOGY
(2022)
Review
Genetics & Heredity
Michal Jeseta, Jana Navratilova, Katerina Franzova, Sandra Fialkova, Bartozs Kempisty, Pavel Ventruba, Jana Zakova, Igor Crha
Summary: Male fertility has been deteriorating globally, especially in countries with high levels of endocrine disruptors. These chemicals can impact hormone receptors, affecting physiological processes and human reproduction, leading to the increasing incidence of male infertility.
FRONTIERS IN GENETICS
(2021)
Article
Environmental Sciences
Eszter Simon, Anja Duffek, Cordula Stahl, Manfred Frey, Marco Scheurer, Jochen Tuerk, Linda Gehrmann, Sarah Konemann, Kees Swart, Peter Behnisch, Daniel Olbrich, Francois Brion, Selim Ait-Aissa, Robert Pasanen-Kase, Inge Werner, Etienne L. M. Vermeirssen
Summary: The study evaluated the implementation of EBMs in the Water Framework Directive through a large-scale sampling and analysis campaign across Europe, generating high-quality chemical and effect-based monitoring data for selected substances. The results demonstrated the excellent screening capability of EBMs, with lower non-quantifiable data compared to conventional analytical methods.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Environmental Sciences
Nicolas Creusot, Betty Chaumet, Melissa Eon, Nicolas Mazzella, Aurelie Moreira, Soizic Morin
Summary: Freshwater biofilms have been increasingly used in ecotoxicology over the past decade to assess environmental stress effects. Untargeted metabolomics is a useful approach for understanding the response of these microbial communities to chemical stress and environmental factors. The study demonstrates the influence of temperature, photoperiod, and flow on the metabolomics response, highlighting the complexity of the relationship between molecular alterations and ecosystem functioning. More research is needed to fully decipher the metabolomics response of freshwater biofilms to chemical stress.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Audrey Catteau, Jean-Marc Porcher, Anne Bado-Nilles, Isabelle Bonnard, Marc Bonnard, Arnaud Chaumot, Elise David, Odile Dedourge-Geffard, Laurence Delahaut, Nicolas Delorme, Adeline Francois, Laura Garnero, Christelle Lopes, Katherine Nott, Patrice Noury, Olivier Palluel, Melissa Palos-Ladeiro, Herve Queau, Sebastien Ronkart, Khadija Sossey-Alaoui, Cyril Turies, Bernard Tychon, Olivier Geffard, Alain Geffard
Summary: The study demonstrated that a multispecies active biomonitoring approach can improve the diagnostic of aquatic systems, and the use of a multispecies Integrated Biomarker Response (IBR) value can assess the quality of water. By studying different species, the study highlighted the importance of a multispecies approach in evaluating biological impacts and contaminant toxicity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Correction
Marine & Freshwater Biology
Julie De Oliveira, Edith Chadili, Benjamin Piccini, Cyril Turies, Emmanuelle Maillot-Marechal, Olivier Palluel, Patrick Pardon, Helene Budzinski, Xavier Cousin, Francois Brion, Nathalie Hinfray
AQUATIC TOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Laura Gence, Danielle Fernezelian, Matthieu Bringart, Bryan Veeren, Armelle Christophe, Francois Brion, Olivier Meilhac, Jean-Loup Bascands, Nicolas Diotel
Summary: This study investigates the biological properties of the traditional medicinal plant Hypericum lanceolatum Lam., revealing its antioxidant, pro-regenerative, anti-lipid accumulation, and pro-neurogenic effects. The results support the potential use of this plant as an alternative treatment for metabolic disorders.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Diego Crespo, Kai Ove Skaftnesmo, Erik Kjaerner-Semb, Ozlem Yilmaz, Birgitta Norberg, Sara Olausson, Petra Vogelsang, Jan Bogerd, Lene Kleppe, Rolf B. Edvardsen, Eva Andersson, Anna Wargelius, Tom J. Hansen, Per Gunnar Fjelldal, Ruediger W. Schulz
Summary: Precocious male maturation in Atlantic salmon aquaculture leads to reduced welfare and increased production costs. Research on the regulation of follicle-stimulating hormone (Fsh) production in male salmonids revealed up-regulation of gonadotropin-related genes in the pituitaries of maturing males exposed to certain environmental conditions. Ex vivo studies showed the influence of hormones and growth factors on gene expression levels in pituitaries from both immature and maturing males.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Amelie Cant, Marc Bonnard, Jean-Marc Porcher, Jean Prygiel, Audrey Catteau, Laurence Delahaut, Olivier Palluel, Cyril Turies, Alain Geffard, Anne Bado-Nilles
Summary: This study conducted biological monitoring of genotoxicity and erythrocyte necrosis in six stations in northern France using a multi-biomarker approach. The results showed that this method can improve the existing biomarker approach and expand the understanding of the potential effects of contaminants.
Review
Biochemistry & Molecular Biology
Kevin Bernal, Charbel Touma, Chedi Erradhouani, Talia Boronat-Belda, Lucas Gaillard, Sara Al Kassir, Helene Le Mentec, Corinne Martin-Chouly, Normand Podechard, Dominique Lagadic-Gossmann, Sophie Langouet, Francois Brion, Anja Knoll-Gellida, Patrick J. Babin, Iva Sovadinova, Pavel Babica, Karine Andreau, Robert Barouki, Jan Vondracek, Paloma Alonso-Magdalena, Etienne Blanc, Min Ji Kim, Xavier Coumoul
Summary: The prevalence of metabolic diseases is increasing and there is evidence to suggest that the chemical environment plays a role in their pathogenesis. This review explores the biological processes involved in the development of metabolic diseases and proposes an integrated pathophysiological viewpoint. The need to improve testing and assessment of endocrine disruptors to prevent and manage metabolic diseases is also discussed.
Article
Fisheries
L. Kleppe, P. G. Fjelldal, E. Andersson, T. Hansen, M. Sanden, A. Bruvik, K. O. Skaftnesmo, T. Furmanek, E. Kjaerner-Semb, D. Crespo, S. Flavell, A. O. Pedersen, P. Vogelsang, A. Torsvik, K. A. Kvestad, S. Olausson, B. Norberg, R. W. Schulz, J. Bogerd, N. Santi, R. B. Edvardsen, A. Wargelius
Summary: Using germ cell-free (GCF), sterile, dnd-knockout salmon for farming can solve the problems associated with precocious maturation and genetic intmgression of farmed breeds into wild populations. The GCF phenotype of salmon is similar to wild type (WT) counterparts in terms of growth and welfare, with the advantage of never maturing.
Article
Environmental Sciences
Pierre-Andre Billat, Celine Brochot, Francois Brion, Remy Beaudouin
Summary: This study used the zebrafish eleutheroembryo model to assess the toxicity and developmental adverse effects of xenobiotics. A new physiologically based pharmacokinetic (PBPK) model was used to predict the concentration of xenobiotics in the eleutheroembryo tissues. The model was improved by considering factors such as dynamic flows through the embryo, metabolic clearance, and experimental temperature. The model showed good agreement with experimental values and provides a tool for designing new assays and evaluating actual exposure.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Lionel Mendes Dias, Mark J. de Keijzer, Daniel Ernst, Farangis Sharifi, Daniel J. de Klerk, Tony G. Kleijn, Emilie Desclos, Jakub A. Kochan, Lianne R. de Haan, Leonardo P. Franchi, Albert C. van Wijk, Enzo M. Scutigliani, Marcel H. Fens, Arjan D. Barendrecht, Jose E. B. Cavaco, Xuan Huang, Ying Xu, Weiwei Pan, Marjo J. den Broeder, Jan Bogerd, Rudiger W. Schulz, Kitty C. Castricum, Victor L. Thijssen, Shuqun Cheng, Baoyue Ding, Przemek M. Krawczyk, Michal Heger
Summary: The study developed a comprehensive tumor targeting and photosensitizer delivery platform. It was found that photodynamically active PSs effectively photosensitized cancer cells and non-cancerous cells without notable systemic toxicity. ZnPC and AlPC delivered by ITLs demonstrated strong tumor-killing capacity in human breast cancer xenografts.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Environmental Sciences
Emmanuelle Lebeau-Roche, Gaelle Daniele, Aurelie Fildier, Christelle Bonnefoy, Cyril Turies, Anne Bado-Nilles, Jean-Marc Porcher, Odile Dedourge-Geffard, Emmanuelle Vulliet, Alain Geffard
Summary: This study applied metabolomics-based approaches to identify toxicity pathways and potential biomarkers in the fish species three-spined stickleback exposed to diclofenac. The results showed significant impacts on the liver metabolome, with upregulation of energy metabolism in females and downregulation in males. The antioxidant system was impacted in males, while the immunity system was down-regulated in females. Several potential metabolic biomarkers were identified.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agriculture, Dairy & Animal Science
Sandra Navarro, Diego Crespo, Rudiger W. Schulz, Wei Ge, Josep Rotllant, Jose Miguel Cerda-Reverter, Ana Rocha
Summary: Reproduction in both males and females is complex and controlled by various hormonal systems that communicate with the hypothalamic-pituitary-gonadal (HPG) system. This study provides new insights into the effects of melanocortin system on gonadal physiology, highlighting the modulatory role of ACTH and MSH on steroid secretion and the need for further research to fully understand the impact of melanocortins on reproductive function. Our results suggest that melanocortins play a role in regulating vitellogenesis in females and testosterone production in males, indicating a potential endocrine involvement in the reproductive system.
Article
Cell Biology
F. L. Almeida, K. O. Skaftnesmo, E. Andersson, L. Kleppe, R. B. Edvardsen, B. Norberg, P. G. Fjelldal, T. J. Hansen, R. W. Schulz, A. Wargelius
Summary: The genetic introgression of farmed salmon into wild populations can harm genetic integrity. To address this issue, researchers have identified the potential role of the Piwil1 protein in germ cell survival in Atlantic salmon. By inducing mutations in Piwil1 using CRISPR-Cas9, they observed a mosaic pattern of mutations in the F0 generation of salmon. These mutants still carried germ cells but exhibited partial germ cell loss and delays in spermatogenesis. Further analysis revealed the importance of the N domain of Piwil1 for its function. By generating F1 fish with different combinations of mutated alleles, they found that complete loss of Piwil1 led to the absence of germ cells, while heterozygous fish displayed an intermediate phenotype with a higher ratio of somatic cells to germ cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Manon Lesage, Manon Thomas, Thierry Pecot, Tu -Ky Ly, Nathalie Hinfray, Remy Beaudouin, Michelle Neumann, Robin Lovell-Badge, Jerome Bugeon, Violette Thermes
Summary: Computational analysis of bio-images using deep learning algorithms has progressed significantly and become more accessible to non-specialists thanks to ready-to-use tools. Recent developments in three-dimensional (3D) imaging of ovaries have also benefited the study of oogenesis mechanisms and female reproductive success. However, analyzing these complex datasets has been a challenge due to the lack of efficient workflows. In this study, we integrated two open-source DL tools, Noise2Void and Cellpose, into an analysis pipeline for 3D follicular content analysis, which showed successful application in different types of ovaries. This pipeline enables automatic and accurate quantification of irregularly stained, low autofluorescence, or heterogeneous follicles sizes in 3D images.
