期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 52, 期 18, 页码 10408-10415出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.8b02308
关键词
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资金
- National Science Foundation (NSF) BRIGE Award [1329576]
- NSF PIRE Award [1545756]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1329576] Funding Source: National Science Foundation
Determining the influence of higher order structure on UVC photolysis will help inform predictions of nucleic acid fate and microorganism inactivation. We measured the direct UV254 photolysis kinetics of four model viral genomes composed of single-stranded and double-stranded RNA (ssRNA and dsRNA, respectively), as well as single-stranded and double-stranded DNA (ssDNA and dsDNA, respectively), in ultrapure water, in phosphate buffered saline (PBS), and encapsidated in their native virus particles. The photolysis rate constants of naked nucleic acids measured by qPCR (RT-qPCR for RNA) and normalized by the number of bases measured in a particular sequence exhibited the following trend: ssDNA > ssRNA approximate to dsDNA > dsRNA. In PBS, naked ssRNA bases reacted, on average, 24X faster than the dsRNA bases, whereas naked ssDNA bases reacted 4.3x faster than dsDNA bases. Endogenous indirect photolysis involving O-1(2) and center dot OH was ruled out as a major contributing factor in the reactions. A comparison of our measured rate constants with rate constants reported in the literature shows a general agreement among the nucleic acid UV254 direct photolysis kinetics. Our results underscore the high resistance of dsRNA to UVC photolysis and demonstrate the role that nucleic acid structure and solution chemistry play in photoreactivity.
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