Article
Chemistry, Analytical
ZhiHang Zhang, Jin Wang
Summary: An optimized anisotropic plasmonic sheet-core-satellite superstructure can achieve sensitive detection of MC-LR toxin by controlling various parameters.
Article
Chemistry, Analytical
Liang Fan, Gang Xiao, Mingliang Wang, Shuang Zhao, Qiaochun Yang, Linyang Cheng, Jinhui Jeanne Huang, Zhao Yue
Summary: By optimizing experimental conditions and introducing different nanomaterials, the sensing properties of PEC immunosensors based on MUA-AuNCs were successfully enhanced. Among them, MUA-AuNCs/cGO showed the best sensing performance.
ANALYTICA CHIMICA ACTA
(2021)
Article
Environmental Sciences
Sijia Hua, Jiawen Chen, Liang Wu, Xinyue Yu, Jing Ye, Yuanting Li, Yongqiang Zhu, Fuxiang Tian
Summary: This study detected the contents of MC-LR in Microcystis aeruginosa cultures and water samples from the Huangpu River using ELISA and HPLC methods. The results showed that ELISA was a reliable and simple method for monitoring low levels of MC-LR. The concentration of MC-LR in the Huangpu River varied with the season, but remained below the WHO drinking water quality standard.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Analytical
Yujie Han, Yu Du, Kun Xu, Xiang Ren, Guanhui Zhao, Zhuangzhuang Ru, Yue Jia, Qin Wei
Summary: An electrochemiluminescence-electrochemistry (ECL-EC) dual-mode biosensing platform was developed to detect Microcystin-LR (MC-LR) with high sensitivity. The platform utilized sulfur quantum dots (SQDs) as an ECL cathode emitter and Ag+ as an ECL signal-amplifying molecule. The biosensor achieved detection in the range of 0.001-100 pg/mL MC-LR concentration with detection limits of 0.17 and 0.24 pg/mL for ECL and EC, respectively.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Hamid Aghamohammadi, Kathryn E. Thomas, Sanjana Srikant, Jason Deglint, Alexander Wong, Mahla Poudineh
Summary: To address the need for rapid detection of cyanotoxins in water resources, an easy-to-use and miniaturized system was developed. A bead-based, competitive fluorescence assay was presented for multiplexed detection of microcystin-LR (MC-LR) and okadaic acid (OA). A reusable microfluidic device, called toxin-chip, was designed for automated detection, which included a micromixer for efficient mixing and a detection chamber for magnetic retainment of beads. Quantum dots (QDs) were used as reporter molecules to enhance sensitivity, and the emitted fluorescence signal was inversely proportional to the toxin concentration. The device showed a low detection limit for both MC-LR and OA, and demonstrated chemical specificity against potential interfering toxins. The toxin-chip holds promise as a versatile and simple tool for cyanotoxin detection.
Article
Chemistry, Analytical
Liqian Zhang, Hang Dong, Haimei Li, Baowei Li, Guangwei Zhao, Hongling Cai, Limei Chen, Jinhua Dong
Summary: Two reliable and effective fluorescent sensors were developed for rapid detection of microcystin in surface water, using a combination of antibodies and fluorescent dyes, successfully achieving the detection of MC-LR.
MICROCHEMICAL JOURNAL
(2021)
Article
Environmental Sciences
Lan Yao, Lingzhi He, Yuesuo Yang, Yu Zhang, Zairan Liu, Lina Liang, Yunxian Piao
Summary: A portable, cheap, and sensitive paper-based electrochemical immunosensor utilizing conductive nanobiochar paper was developed for sensitive detection of microcystin-LR toxin in water. The sensor showed specific quantification of MCLR with a response time of less than 5 minutes, and achieved a lowest detection limit of 17 pM. The sensor exhibited high selectivity, reproducibility, and storage stability, making it a promising tool for cost-effective electrochemical device development.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Engineering, Environmental
Huixia Zhang, Bingyan Li, Yipeng Liu, Huiyan Chuan, Yong Liu, Ping Xie
Summary: This review focuses on the immunoassay detection of MC-LR in the past two decades, including classical ELISA, modern immunoassay with optical signal, and modern immunoassay with electrical signal. The water environment was predominantly used as the main detection system, with the advantages and disadvantages of different detection methods compared and analyzed. The principles and applications of immunoassays in water samples were elaborated, and current challenges and developmental trends in immunoassay were systematically introduced to enhance MC-LR detection performance.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Sanjay S. Timilsina, Pawan Jolly, Nolan Durr, Mohamed Yafia, Donald E. Ingber
Summary: The team developed an efficient antifouling coating for multiplexed EC sensors, addressing the sensitivity and selectivity loss caused by biological samples, and made significant progress.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Chemistry, Analytical
Liang Fan, Jinhui Jeanne Huang, Jianjun Liao
Summary: The competitive smartphone-based portable electrochemical aptamer sensing system offers portability, low cost, and fast response, meeting the demand for on-site detection of MC-LR.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Jeong Ah Park, Yein Kwon, Xuan Ai Le, Trung Hieu Vu, Hanbin Park, Hoseok Lee, Hye Kyu Choi, Chulhwan Park, Moon Il Kim, Taek Lee
Summary: This study developed a rapid electrochemical biosensor using nanozymes and aptamers for the detection of microcystin-LR (MC-LR). The detection time was reduced to 10 minutes using alternating current electrothermal flow (ACEF). The sensitivity of MC-LR detection was improved by using MnO2/MC-LR aptamer conjugates. The study achieved an LOD of 3.36 pg mL(-1) in the linear concentration range of 10 pg mL(-1) to 1 mu g mL(-1).
Article
Chemistry, Analytical
Xiaoqian Wei, Shanlin Wang, Yujuan Zhan, Tianhan Kai, Ping Ding
Summary: We report a methylene blue-modified electrochemical aptamer sensor for determining microcystin-LR. The sensor signal transduction is based on changes in conformation and position of methylene blue induced by binding with microcystin-LR. We optimized the parameters and evaluated the sensor's electrochemical performance using square wave voltammetry. The reliability of the method was verified by testing environmental samples, and the sensor was shown to be reusable and regenerable with good accuracy and reproducibility. This rapid, reagent-free, and sensitive sensing platform will facilitate routine monitoring of microcystin-LR.
Article
Engineering, Environmental
Jinghua Liu, Feng Tan, Yifei Xing, Qian Zhang, Zhanyi Zhao, Xiaochun Wang, Yan Wang, Hongxia Zhao
Summary: This study proposes a label-free chemiresistive sensor for the detection of microcystin (MC)-LR in water samples. The sensor shows high sensitivity and selectivity, with a wide linear range and stable detection. It can be used for monitoring MC-LR released by microcystis aeruginosa and measuring MC-LR in freshwater lakes with moderate algal blooms.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Gu Shi, Chong Yan, Junhua Chen
Summary: Researchers developed an ultrasensitive aptasensor for the detection of microcystin-LR based on the assembly of Y-shaped hairpins. The cyclic assembly reactions resulted in a high fluorescence signal, enabling the detection of microcystin-LR with a low limit of 0.2 pM.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biophysics
Yuan Wang, Yuye Li, Chang Liu, Na Dong, Dong Liu, Tianyan You
Summary: A laser-induced graphene (LIG)-based electrochemical aptasensor was developed for sensitive on-site detection of microcystin-LR (MC-LR). The LIG electrode, prepared via thermal transfer, acted as a quasireference electrode and provided a large surface area for signal amplification. The aptasensor showed high analytical performance for MC-LR detection with a linear range of 1 x 10-2 - 1 x 105 pM and a detection limit of 3 x 10-3 pM, making it suitable for water sample analysis with comparable reliability and accuracy to the standard method. Additionally, a portable detection platform was constructed for on-site detection of MC-LR.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Nik J. Walch, Frank Davis, Nathan Langford, Joanne L. Holmes, Stuart D. Collyer, Seamus P. J. Higson
ANALYTICAL CHEMISTRY
(2015)
Article
Chemistry, Physical
Rifat Capan, Hilal Goktas, Zikriye Ozbek, Sibel Sen, Mehmet Emin Ozel, Frank Davis
APPLIED SURFACE SCIENCE
(2015)
Article
Nanoscience & Nanotechnology
Nik J. Walch, Alexei Nabok, Frank Davis, Seamus P. J. Higson
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
(2016)
Article
Chemistry, Analytical
Eva L. Tur-Garcia, Frank Davis, Stuart D. Collyer, Joanne L. Holmes, Hugh Barr, Seamus P. J. Higson
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Chemistry, Analytical
Jiri Hromadka, Sergiy Korposh, Matthew C. Partridges, Stephen W. James, Frank Davis, Derrick Crump, Ralph P. Tatam
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Biophysics
William S. Roberts, Frank Davis, Joanne L. Holmes, Stuart D. Collyer, Lee D. Larcombe, Sarah L. Morgan, Seamus P. J. Higson
BIOSENSORS & BIOELECTRONICS
(2013)
Article
Biophysics
Timothy R. J. Holford, Joanne L. Holmes, Stuart D. Collyer, Frank Davis, Seamus P. J. Higson
BIOSENSORS & BIOELECTRONICS
(2013)
Article
Mechanics
Matthew Partridge, Frank Davis, Stephen W. James, Ralph P. Tatam, Seamus P. J. Higson
FLUID DYNAMICS RESEARCH
(2014)
Article
Chemistry, Applied
Laura Mercantili, Frank Davis, Seamus P. J. Higson
JOURNAL OF SURFACTANTS AND DETERGENTS
(2014)
Article
Engineering, Chemical
Laura Mercantili, Frank Davis, Stuart D. Collyer, Seamus P. J. Higson
LUBRICATION SCIENCE
(2015)
Article
Materials Science, Multidisciplinary
Rifat Capan, Tim Richardson, Aseel K. Hassan, Frank Davis
MATERIALS CHEMISTRY AND PHYSICS
(2014)
Article
Materials Science, Multidisciplinary
Matthew Partridge, Rebecca Wong, Mike Collins, Stephen W. James, Frank Davis, Ralph P. Tatam, Seamus P. J. Higson
MATERIALS CHEMISTRY AND PHYSICS
(2014)
Article
Chemistry, Analytical
Matthew Partridge, Rebecca Wong, Stephen W. James, Frank Davis, Seamus P. J. Higson, Ralph P. Tatam
SENSORS AND ACTUATORS B-CHEMICAL
(2014)
Article
Materials Science, Multidisciplinary
Kieran Deasy, Khalid N. Sediq, Stuart Brittle, Tao Wang, Frank Davis, Tim H. Richardson, David G. Lidzey
JOURNAL OF MATERIALS CHEMISTRY C
(2014)
Proceedings Paper
Engineering, Electrical & Electronic
Jiri Hromadka, Stephen James, Frank Davis, Ralph P. Tatam, Derrick Crump, Sergiy Korposh
24TH INTERNATIONAL CONFERENCE ON OPTICAL FIBRE SENSORS
(2015)