Article
Environmental Sciences
Shiwen Deng, Caifeng Li, Junqi Chen, Zhao Cui, Tong Lei, Hongjun Yang, Peng Chen
Summary: This study evaluated the effects of TCS exposure on human stem cells and its molecular mechanisms. It was found that TCS inhibited cell proliferation and differentiation, induced senescence, mitochondrial dysfunction, ER stress, and oxidative stress, and significantly induced apoptosis and autophagy flux inhibition in high concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Lucia Gerbi, Christine Austin, Nicolo Foppa Pedretti, Nia McRae, Chitra J. Amarasiriwardena, Adriana Mercado-Garcia, Libni A. Torres-Olascoaga, Martha M. Tellez-Rojo, Robert O. Wright, Manish Arora, Colicino Elena
Summary: This study assessed the relationship between child tooth dentine and maternal blood lead measurements, and found a strong correlation between them. Maternal lead exposure during pregnancy can be estimated using child tooth dentine profiles. Biomarkers can effectively evaluate maternal lead exposure.
ENVIRONMENT INTERNATIONAL
(2022)
Review
Biochemistry & Molecular Biology
Nurul Hafizah Mohd Nor, Nur Izzati Mansor, Mohd Izhar Ariff Mohd Kashim, Mohd Helmy Mokhtar, Farah Ayuni Mohd Hatta
Summary: Stem cells derived from human exfoliated deciduous teeth (SHED) are a promising stem cell source for regenerative medicine due to their availability, low ethical concerns, and therapeutic potential. SHED have a high proliferation rate and can differentiate into multiple cell types. The therapeutic effects of SHED are mainly mediated by their secretome, which consists of various soluble factors that promote tissue survival and recovery. This review provides an overview of the secretome derived from SHED and its potential applications in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ryo Kunimatsu, Kodai Rikitake, Yuki Yoshimi, Nurul Aisyah Rizky Putranti, Yoko Hayashi, Kotaro Tanimoto
Summary: This study aimed to compare the effects of CD146 on cell proliferative and substrate metabolic abilities in a population of SHED. The results showed that CD146+ SHED had higher osteogenic differentiation potential compared to the other groups. CD146 may be a valuable population of cells for bone regeneration therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
S. M. Ziauddin, Misako Nakashima, Hideto Watanabe, Michiyo Tominaga, Koichiro Iohara
Summary: This study compared the stem cell properties and pulp regenerative potential of DT-DPSCs and PT-DPSCs from the same individual. The results showed that DT-DPSCs exhibited higher expression of stem cell markers and angiogenic factors compared to PT-DPSCs. DT-DPSC-derived conditioned medium also promoted increased cell proliferation, migration, and angiogenesis. Furthermore, the regenerated pulp tissues in both DT-DPSCs and PT-DPSCs groups were similar. Therefore, DT-DPSCs could be a potential alternative to PT-DPSCs for pulp regenerative therapy.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Nathalia C. Oliveira, Fabiele B. Russo, Patricia Beltrao-Braga
Summary: This study aimed to establish a method for differentiating sensory neurons from induced pluripotent stem cells (iPSCs) derived from human exfoliated deciduous teeth (SHED), which have the same embryonic origin as the peripheral nervous system (PNS). The protocol successfully differentiated neural crest and PNS cells from SHED-derived iPSCs, providing a platform for future studies on PNS pathologies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Forough Mahdavi-Jouibari, Benyamin Parseh, Ezatolah Kazeminejad, Ayyoob Khosravi
Summary: Cartilage lesions are common and challenging to regenerate. Stem cell-based therapies, especially using Mesenchymal stem cells (MSCs) and Stem cells from human exfoliated deciduous teeth (SHED), offer promising solutions for cartilage regeneration. MSCs can differentiate into mature chondrocytes and engraft into the host, while SHED-derived secretome contains biomolecules that promote tissue regeneration, including cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Anatomy & Morphology
Huacui Xiong, Ke Chen
Summary: Isolation of De-APDCs from the apical pulp of deciduous teeth with an immature apex showed higher clonogenic and proliferative ability as well as osteo/dentinogenic differentiation capacity compared to De-DPCs. The induced De-APDCs also exhibited potential for differentiation into adipogenic, neurogenic, and chondrogenic lineages. Transplantation of De-APDCs into HA/TCP scaffolds in nude mice successfully regenerated dentin/pulp-like structures. It indicates the accessibility and potential of De-APDCs as a cell source for tooth regeneration.
Article
Environmental Sciences
Alexa Friedman, Julia Anglen Bauer, Christine Austin, Timothy J. Downs, Yorghos Tripodis, Wendy Heiger-Bernays, Roberta F. White, Manish Arora, Birgit Claus Henn
Summary: This study characterized early-life metal exposure in a community concerned about past exposures using deciduous teeth as a noninvasive biomarker, demonstrating their utility in providing retrospective information on dose and timing of exposure at high resolution.
JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Priscila A. Giovani, Luciane Martins, Cristiane R. Salmon, Luciana S. Mofatto, Adriana F. P. Leme, Regina M. Puppin-Rontani, Tamara N. Kolli, Brian L. Foster, Francisco H. Nociti, Kamila R. Kantovitz
Summary: Proteomic analysis of dental cementum revealed clear differences between deciduous and permanent teeth, providing new insights into developmental disparities and physiological/pathological events such as root resorption. Studies showed distinct protein composition in deciduous and permanent dental cementum, highlighting potential implications for dental attachment and pathology.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Eduarda R. Oliveira, Leonara Fayer, Rafaella S. S. Zanette, Luiz O. Ladeira, Luiz F. C. de Oliveira, Carlos M. C. Maranduba, Humberto M. Brandao, Michele Munk
Summary: Carboxylated multi-wall carbon nanotubes (MWCNT-COOH) have no toxic effects on human stem cell viability, making them potentially useful in tissue engineering and regenerative medicine.
Article
Cell & Tissue Engineering
Xiaofeng Bai, Xuedi Zhang, Chun Wang, Yao Liu, Xuemei Liu, Yu Fan, Xia Zhang
Summary: The study found that systemic or local injection of SHED can reduce the sensitivity of rats to mechanical stimuli after nerve injury, and this effect persists throughout the 8-week observation period. PKH26-labeled SHED were distributed to the ipsilateral trigeminal ganglions 24 and 72 hours after local injection. Transplanting SHED at the lesion site led to reduced inflammatory cell infiltration and levels of proinflammatory cytokines in the injured nerve, and inhibited the early-phase upregulation of TRPV1 expression in the trigeminal nerve and ganglion induced by CCI-ION.
STEM CELLS INTERNATIONAL
(2021)
Article
Immunology
Yasuhiro Katahira, Fumihiro Murakami, Shinya Inoue, Satomi Miyakawa, Eri Sakamoto, Yuma Furusaka, Aruma Watanabe, Ami Sekine, Masahiko Kuroda, Hideaki Hasegawa, Izuru Mizoguchi, Takayuki Yoshimoto
Summary: Pressure ulcers (PUs) are a growing problem worldwide, especially with the aging population, but there are no effective treatments available. This study investigated the therapeutic potential of conditioned medium (CM) derived from immortalized stem cells (SHED) on PU formation induced by ischemia-reperfusion injury. The results showed that CM from SHEDs promoted angiogenesis and increased oxidative stress resistance, leading to therapeutic effects on PU formation. Further research is needed to meet regulatory requirements and ensure the safety and effectiveness of CM-based therapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Fumiya Kano, Noboru Hashimoto, Yao Liu, Linze Xia, Takaaki Nishihara, Wakana Oki, Keita Kawarabayashi, Noriko Mizusawa, Keiko Aota, Takayoshi Sakai, Masayuki Azuma, Hideharu Hibi, Tomonori Iwasaki, Tsutomu Iwamoto, Nobuyasu Horimai, Akihito Yamamoto
Summary: Radiation therapy for head and neck cancers can cause severe radiation-induced xerostomia. Conditioned medium derived from stem cells from human exfoliated deciduous teeth (SHED-CM) was found to prevent ulcer formation and maintain salivary gland function. SHED-CM enhanced antioxidant gene expression and suppressed oxidative stress. The study suggests that SHED-CM may provide substantial therapeutic benefits for radiation-induced xerostomia.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Aleksandra Jaukovic, Tamara Kukolj, Drenka Trivanovic, Ivana Okic-Dordevic, Hristina Obradovic, Maja Miletic, Vanja Petrovic, Slavko Mojsilovic, Diana Bugarski
Summary: The study found that IL-17 and bFGF have different effects on the proliferation and clonogenicity of dental pulp stem cells from primary and permanent teeth, as well as on the expression of pluripotency markers, while showing similar effects on differentiation capacity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Environmental Sciences
Julia Anglen Bauer, Birgit Claus Henn, Christine Austin, Silvia Zoni, Chiara Fedrighi, Giuseppa Cagna, Donatella Placidi, Roberta F. White, Qiong Yang, Brent A. Coull, Donald Smith, Roberto G. Lucchini, Robert O. Wright, Manish Arora
ENVIRONMENT INTERNATIONAL
(2017)
Article
Environmental Sciences
Andrea E. Cassidy-Bushrow, Alexandra R. Sitarik, Suzanne Havstad, Sung Kyun Park, Lawrence F. Bielak, Christine Austin, Christine Cole Johnson, Manish Arora
ENVIRONMENT INTERNATIONAL
(2017)
Article
Multidisciplinary Sciences
Manish Arora, Abraham Reichenberg, Charlotte Willfors, Christine Austin, Chris Gennings, Steve Berggren, Paul Lichtenstein, Henrik Anckarsater, Kristiina Tammimies, Sven Bolte
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Paul Curtin, Christine Austin, Austen Curtin, Chris Gennings, Manish Arora, Kristiina Tammimies, Charlotte Willfors, Steve Berggren, Paige Siper, Dheeraj Rai, Kristin Meyering, Alexander Kolevzon, Josephine Mollon, Anthony S. David, Glyn Lewis, Stanley Zammit, Lynne Heilbrun, Raymond F. Palmer, Robert O. Wright, Sven Bolte, Abraham Reichenberg
Article
Environmental Sciences
Megan K. Horton, Leon Hsu, Birgit Claus Henn, Amy Margolis, Christine Austin, Katherine Svensson, Lourdes Schnaas, Chris Gennings, Howard Hu, Robert Wright, Martha Maria Tellez Rojo, Manish Arora
ENVIRONMENT INTERNATIONAL
(2018)
Article
Multidisciplinary Sciences
Milo R. Smith, Priscilla Yevoo, Masato Sadahiro, Christine Austin, Chitra Amarasiriwardena, Mahmoud Awawda, Manish Arora, Joel T. Dudley, Hirofumi Morishita
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Tanya M. Smith, Christine Austin, Daniel R. Green, Renaud Joannes-Boyau, Shara Bailey, Dani Dumitriu, Stewart Fallon, Rainer Grun, Hannah F. James, Marie-Helene Moncel, Ian S. Williams, Rachel Wood, Manish Arora
Article
Multidisciplinary Sciences
Nicholas J. Bessman, Jacques R. R. Mathieu, Cyril Renassia, Lei Zhou, Thomas C. Fung, Keith C. Fernandez, Christine Austin, Jesper B. Moeller, Sara Zumerle, Sabine Louis, Sophie Vaulont, Nadim J. Ajami, Harry Sokol, Gregory G. Putzel, Tara Arvedson, Robbyn E. Sockolow, Samira Lakhal-Littleton, Suzanne M. Cloonan, Manish Arora, Carole Peyssonnaux, Gregory F. Sonnenberg
Article
Biochemistry & Molecular Biology
Richard E. Frye, Janet Cakir, Shannon Rose, Leanna Delhey, Sirish C. Bennuri, Marie Tippett, Stepan Melnyk, S. Jill James, Raymond F. Palmer, Christine Austin, Paul Curtin, Manish Arora
Summary: By investigating the role of mitochondria in the influence of prenatal air pollution exposure on neurodevelopment and behavior in children with ASD, it was found that mitochondrial respiration is influenced by PM2.5 exposure during childhood, with varying effects on children with and without NDR. Mitochondrial respiration linked to energy production accounted for a portion of the effect of average prenatal PM2.5 exposure on neurodevelopment and behavioral symptoms. Structural equation models estimated that PM2.5 and mitochondrial respiration contribute to the effect on neurodevelopment, while behavior is directly influenced by prenatal PM2.5 exposure.
MOLECULAR PSYCHIATRY
(2021)
Article
Neurosciences
Julia Anglen Bauer, Roberta F. White, Brent A. Coull, Christine Austin, Manuela Oppini, Silvia Zoni, Chiara Fedrighi, Giuseppa Cagna, Donatella Placidi, Stefano Guazzetti, Qiong Yang, David C. Bellinger, Thomas F. Webster, Robert O. Wright, Donald Smith, Megan Horton, Roberto G. Lucchini, Manish Arora, Birgit Claus Henn
Summary: Understanding the neurodevelopmental effects of manganese is complex due to its essentiality, with studies showing inconsistent associations between excess manganese and child cognition. This study aimed to estimate prospective associations between manganese measured in different early-life time windows and adolescent cognition, revealing timing-dependent effects and differing associations across time windows.
Article
Anthropology
Tanya M. Smith, Christine Austin, Janaina N. Avila, Wendy Dirks, Daniel R. Green, Ian S. Williams, Manish Arora
Summary: This study presented a model for identifying nursing behavior in primates based on changes in barium concentration in teeth. By comparing pre-and postnatal trends in barium, zinc, strontium, and oxygen, the researchers found that barium and zinc were consistent biomarkers of nursing initiation. Oxygen isotope compositions also fluctuated rapidly within two weeks of birth, indicating changes in infant body water. The findings of this study have implications for bioarchaeological studies and public health investigations.
JOURNAL OF ARCHAEOLOGICAL SCIENCE
(2022)
Article
Chemistry, Analytical
Mauro Martinez, G. Jean Harry, Erin N. Haynes, Pi-I. D. Lin, Emily Oken, Megan K. Horton, Robert O. Wright, Manish Arora, Christine Austin
Summary: In this work, the use of laser induced breakdown spectroscopy (LIBS) to obtain quantitative fluoride distribution images of teeth using the molecular emission of calcium fluoride (CaF) is proposed. The method was validated using a rat model and human teeth samples, showing its applicability to study tooth-fluoride exposure, especially in children.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Medicine, General & Internal
Paul Curtin, Janina Neufeld, Austen Curtin, Christine Austin, Johan Isaksson, Karl Lundin Remnelius, Hjalmar Nobel Norrman, Manish Arora, Sven Bolte
Summary: Recent advances in exposure biology suggest that biomarkers of elemental uptake and metabolism measured in hair samples can predict autism diagnosis. In a study sample including twin pairs with concordant and discordant diagnoses, the analysis showed associations between elemental biomarkers in hair and functional connectivity in the default mode network (DMN), particularly in zinc metabolism. These findings indicate that functional dynamics in elemental metabolism are related to persistent patterns of functional connectivity in the DMN and are altered in autism.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Christine Austin, Paul Curtin, Manish Arora, Abraham Reichenberg, Austen Curtin, Miyuki Iwai-Shimada, Robert O. Wright, Rosalind J. Wright, Karl Lundin Remnelius, Johan Isaksson, Sven Boelte, Shoji F. Nakayama
Summary: Autism spectrum disorder (ASD) is a neurodevelopmental condition that is challenging to diagnose and treat at an early age. Researchers have developed a non-invasive biomarker using mass spectrometry analysis of hair samples, coupled with machine learning, which can predict ASD risk as early as 1 month of age.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Environmental Sciences
Christine Austin, Piyush Kumar, Elizabeth A. Carter, Joonsup Lee, Tanya M. Smith, Katie Hinde, Manish Arora, Peter A. Lay
Summary: The regular secretion of enamel and dentine can be interrupted by stress, leading to the formation of accentuated growth lines. By using Raman spectroscopy, we have found that small biochemical changes along these accentuated lines coincide with medical events and disruptions in weight trajectory in captive macaques. In this study, we applied these techniques to investigate biochemical changes related to illness and medical treatment during early infancy in humans. Chemometric analysis revealed changes in phenylalanine and other biomolecules, indicating stress-induced alterations. Raman spectroscopy mapping of teeth can provide objective and minimally-destructive insights into an individual's stress exposure.