Article
Chemistry, Applied
He Yu, Miao Wang, Jing Cao, Yongxin She, Yongan Zhu, Jiaming Ye, A. M. Abd El-Aty, Ahmet Hacimuftuoglu, Jing Wang, Shuibing Lao
Summary: A dual-mode method based on fluorescent and colorimetric sensor was developed for determination of organophosphate pesticides. The method involves inhibition of color change and fluorescence signal enhancement in the presence of OPs, enabling detection of dichlorvos, trichlorfon, and paraoxon with good recovery rates.
Article
Chemistry, Multidisciplinary
Li Wu, Qijie Mo, Shoulian Wei, Ruiping Gao, Shuai Zhang, Xiaohua Zhu
Summary: This study presents a novel colorimetric method for detecting CLB using Fe3O4@mSiO2@MMT-SDS nanoparticles. The method is simple, fast, cost-efficient, and sensitive, making it suitable for rapid and accurate screening of CLB in pork and animal feeds.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Review
Chemistry, Analytical
Mihaela Puiu, Cristina Nativi, Camelia Bala
Summary: The development of sensitive and selective platforms for cancer biomarker detection is crucial for early diagnosis, disease monitoring, and treatment. This review provides an introduction to tumour-associated antigens (TAAs) and their application in recognizing different types and stages of cancer. The review also discusses the use of electrochemical bio/immunosensors designed for TAA detection in point-of-care (POC) environments, with a focus on the detection of small fragments of TAAs and the feasibility of organic-inorganic hetero-nano-interfaces-based bio/immunosensors for POC applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Sixuan Duan, Tianyu Cai, Jia Zhu, Xi Yang, Eng Gee Lim, Kaizhu Huang, Kai Hoettges, Quan Zhang, Hao Fu, Qiang Guo, Xinyu Liu, Zuming Yang, Pengfei Song
Summary: This paper presents a smartphone platform assisted by deep learning for ultra-accurate testing of paper-based microfluidic colorimetric enzyme-linked immunosorbent assay (c-ELISA). The platform eliminates the influences of random lighting conditions and achieves high accuracy in classifying/predicting the concentration of rabbit IgG. A user-friendly smartphone application has been developed to fully automate the sensing process.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Wei Xu, Jianwen Fei, Wei Yang, Yani Zheng, Yin Dai, Marwan Sakran, Jun Zhang, Wanying Zhu, Junli Hong, Xuemin Zhou
Summary: A novel nanocomposite Fe3O4@MoS2-Au NPs was constructed and a colorimetric/electrochemical dual-mode sensor was successfully prepared for H2O2 detection. The sensor showed a wide linear range, low detection limits, and can be used for detecting H2O2 in normal human serum, with promising application prospects.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Wei Yang, Chenyuan Weng, Xiaoyun Li, Hongliang He, Jianwen Fei, Wei Xu, Xiaoqiang Yan, Wanying Zhu, Hongsong Zhang, Xuemin Zhou
Summary: Artificial mimic enzyme has become a powerful substitute for natural enzyme, with nano-enzymes biosensors being the focus of current research. A novel magnetic ternary nanocomposite, hemin-Fe3O4@polypyrrole, exhibited stronger peroxidase activity than Fe3O4 and h-ppy nanoparticles, making it a promising candidate for biosensor construction.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Shunbi Xie, Heshan Yang, Liumei Teng, Shuting Liang, Jin Zhang, Ying Tang, Yi Xu
Summary: In this study, iron-based metal organic frameworks were used to produce Prussian blue, which served as a self-sacrifice beacon. A dual-mode electrochemical-colorimetric biosensing platform was established for kanamycin detection. CoFe2O4 magnetic nanobeads were employed for immobilization and separation of the signal beacon, and the combination between V MBs and magnetic electrodes simplified the electrochemical testing process. The dual-mode strategy showed satisfactory specificity and enhanced applicability for kanamycin detection in real samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Environmental Sciences
Jinchuan Liu, Yunpeng Xing, Yongshu Lin, Yangcun Xie, Xiaohong Zhou
Summary: Common cyanotoxins, like microcystins and nodularins, are produced by harmful cyanobacterial algal blooms in freshwater systems. Reliable enzyme-linked immunosorbent assay kits are needed for routine monitoring of cyanotoxins in drinking water and ambient water. We validated the performance of a self-produced assay kit and compared the pretreatment approaches recommended by China and the United States for cyanotoxin detection. The concentrations of cyanotoxins measured using the US approach were 1-5 times higher than those measured using the Chinese approach, indicating that the Chinese approach overlooks cyanotoxins.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemical Research Methods
Pablo Rodriguez-Mateos, Bongkot Ngamsom, Daglus Ameyo, Patrick Wakaba, Clement Shiluli, Alexander Iles, Jesse Gitaka, Nicole Pamme
Summary: Researchers developed a lab-on-a-chip platform for extracting, concentrating, and purifying Neisseria gonorrhoeae DNA, and integrated a colorimetric isothermal amplification assay for detection. The platform could detect as low as 500 copies/mL from spiked synthetic urine and showed no cross-reactivity with other common STIs. The credit card-sized device allows DNA extraction and purification without power or centrifuges, and provides a straightforward positive/negative result within 1 hour. These advantages offer great potential for accurate, affordable, and accessible monitoring of gonorrhea infection in resource-poor settings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jian-Hui Li, Xue-Lei Deng, Yu-Lian Zhao, Xiao-Yan Zhang, Yun-Peng Bai
Summary: This study presents an improved approach to immobilize a malathion degrading enzyme PoOPHM9 on paper, resulting in high enzyme activity capture and rapid colorimetric detection of malathion in a short period of time. The paper-based biosensors showed promising potential for on-site analysis of malathion-contaminated samples.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Lin Long, Mingji Zong, Yunqing Xie, Qing Chai, Jianbo Liu, Xiaochun Wu
Summary: A nanoreactor with a hollow Pt nanocage-encapsulated mesoporous SiO2 shell was fabricated using a facile and general strategy. This structure exhibited highly dispersed catalytic Pt nanoparticles inside the cavity enclosed by the SiO2 shell, providing a framework for efficient catalysis. The nanoreactors demonstrated high sensitivity and detection performance when used as biosensors for specific antibodies.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Xiang Yuan, Huiling Zhang, Guojiang Mao, Lin Wei
Summary: This work presents a simple and label-free single particle detection (SPD) method for accurate quantification of melamine (Mel). By modifying the surface of gold nanoparticles (AuNPs) with MnO2, the color change of the particles allows for quantitative detection. This method shows potential for the detection of chemical contaminants in food.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Rui Pei, Li Ye, Chuanyong Jing
Summary: In this study, the researchers modulated the spatial conformation of arsenite oxidase AioAB by using the metal-organic framework ZIF-8, which enhanced its specificity towards antimonite. The developed electrochemical biosensor, AioAB@ZIF-8, showed a substrate specificity towards antimonite that was one order of magnitude higher than that of arsenite. Raman spectroscopy confirmed the structural changes of AioAB in ZIF-8, indicating the breakage of S-S bond and conversion of α-helix to random coil. This study provides new insights into tuning enzyme specificity and offers a new approach for biosensing metal(loid)s without specific proteins.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Shuping Liu, Xingxing Jiang, Fu Wan, Shengbin Jia, Shihui Si
Summary: In this study, a novel homogeneous electrochemical sensor was proposed for the detection of microRNA-21 (miRNA-21) using functionalized magnetic nanoparticles and enzyme-assisted signal amplification. The sensor showed a linear correlation between the variation in current and miRNA-21 concentration, with a low detection limit of 0.27 aM. The successful detection of miRNA-21 in human serum and different cell lysates demonstrates its potential in biomedical diagnostics and clinical therapeutics.
Article
Chemistry, Analytical
Mengwen Li, Ao Shen, Yimin Hou, Xiaohui Hao, Man Du, Xiaoyu Du, Shufeng Ma, Jiaxin Yuan, Lala Hou, Yongwei Zhao, Ziqi Li, Yunxu Yang
Summary: This study presents a novel MOF-NADH-mediated colorimetric assay for highly sensitive detection of FA. The method is based on the biomimetic enzyme reductive reaction and the co-catalysis of metalloporphyrin with oxidized TMB, offering an excellent linear relationship within a broad range of FA concentrations and a low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Elena Yeregui, Jenifer Masip, Consuelo Vilades, Pere Domingo, Yolanda M. Pacheco, Julia Blanco, Josep Mallolas, Veronica Alba, Montserrat Vargas, Graciano Garcia-Pardo, Eugenia Negredo, Montserrat Olona, Judit Vidal-Gonzalez, Maria Peraire, Anna Marti, Laia Reverte, Frederic Gomez-Bertomeu, Manuel Leal, Francesc Vidal, Joaquim Peraire, Anna Rull
Summary: Low concentrations of adiponectin receptor (APLNR) and zinc-alpha-2-glycoprotein (ZAG) were associated with poor immune recovery in treated PLHIV, indicating potential predictive biomarkers of a discordant immunological response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Panagiota Katikou, Cengiz Gokbulut, Ali Riza Kosker, Monica Campas, Fatih Ozogul
Summary: Tetrodotoxin is a potent marine toxin found in various marine and terrestrial organisms. Its toxic effects and the lack of an antidote make TTX poisoning a significant global health concern.
Article
Food Science & Technology
Maria Jose Ramos-Sosa, Natalia Garcia-alvarez, Andres Sanchez-Henao, Freddy Silva Sergent, Daniel Padilla, Pablo Estevez, Maria Jose Caballero, Jose Luis Martin-Barrasa, Ana Gago-Martinez, Jorge Diogene, Fernando Real
Summary: The Canary Islands are a hotspot for CTX, and this study found that most fish livers had higher CTX levels than the flesh, and even fish without detectable CTX in the flesh could exhibit liver toxicity. This is the first evidence of C-CTX1 presence in the common two-banded seabream in the Canary Islands.
Article
Chemistry, Medicinal
Angels Tudo, Maria Rambla-Alegre, Cintia Flores, Nuria Sagrista, Paloma Aguayo, Laia Reverte, Monica Campas, Neide Gouveia, Carolina Santos, Karl B. Andree, Antonio Marques, Josep Caixach, Jorge Diogene
Summary: Ciguatera Poisoning (CP) is a public concern in some temperate regions, and this study identified the presence of CTX-like toxicity in fish specimens from Macaronesia. The liver of Muraena augusti had the highest CTX-like toxicity, and CTX analogues were detected in this species as well as Gymnothorax unicolor.
Article
Pharmacology & Pharmacy
Laura A. Uribe, Sandra Leonardo, Thorbjorn Terndrup Nielsen, Casper Steinmann, Monica Campas, Alex Fragoso
Summary: Veratridine is a plant neurotoxin that can form inclusion complexes with cyclodextrins, showing potential for novel research applications.
Article
Immunology
Victoria Ceperuelo-Mallafre, Laia Reverte, Joaquim Peraire, Ana Madeira, Elsa Maymo-Masip, Miguel Lopez-Dupla, Alicia Gutierrez-Valencia, Ezequiel Ruiz-Mateos, Maria Jose Buzon, Rosa Jorba, Joan Vendrell, Teresa Auguet, Montserrat Olona, Francesc Vidal, Anna Rull, Sonia Fernandez-Veledo
Summary: The immune response to SARS-CoV-2 in COVID-19 patients is driven by mitochondrial-associated mechanisms, which can disrupt glucose metabolism and extracellular signaling. This study examined mitochondrial energy-related metabolites as potential biomarkers for predicting the risk of severe COVID-19 illness. The findings suggest that circulating pyruvate levels, along with other clinical factors, can serve as independent predictors of critical COVID-19 outcomes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mounira Alkassar, Sandra Leonardo, Jorge Diogene, Monica Campas
Summary: Marine toxins are potent toxic compounds that need to be detected in seafood to prevent human poisoning. The development of cell-based biosensors for neurotoxins is crucial. This study immobilized Neuro-2a cells on different electrodes and evaluated their presence and viability using MTT detection. The system successfully detected toxicity in standard solutions of CTX1B and TTX, as well as fish extracts containing these toxins.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Applied
Monica Campas, Sandra Leonardo, Maria Rambla-Alegre, Nuria Sagrista, Raquel Vaya, Jorge Diogene, Mabel Torrens, Alex Fragoso
Summary: This study evaluated three materials as clean-up materials for detecting fish flesh extracts containing CTXs and found that γ-CD-HDI in column format showed the best results, improving the sensitivity and reliability of the cell-based assay.
Article
Marine & Freshwater Biology
Giorgio Honsell, Greta Gaiani, Masahiro Hirama, Marco Pelin, Aurelia Tubaro, Takeshi Tsumuraya, Monica Campas
Summary: This study used immunocytochemistry to localize the presence of CTX-like compounds in G. australes. The results showed that these compounds are present in the cytoplasm and are released to cover the cell surface.
Review
Food Science & Technology
Jaume Reverte, Mounira Alkassar, Jorge Diogene, Monica Campas
Summary: This article provides an overview of the detection of ciguatoxins (CTXs) and tetrodotoxins (TTXs) using biorecognition molecules and various assay configurations and transduction strategies. The advantages and limitations of systems based on cells, receptors, antibodies, and aptamers are described, along with identified challenges in marine toxin detection. The validation of these bioanalytical systems through sample analysis and comparison with other techniques is also discussed. These tools have shown promise in detecting and quantifying CTXs and TTXs, making them valuable for research and monitoring programs.
Article
Engineering, Chemical
Dora Cserbik, Paula E. Redondo-Hasselerharm, Maria J. J. Farre, Josep Sanchis, Arantxa Bartolome, Alexandra Paraian, Eva Maria Herrera, Josep Caixach, Cristina M. M. Villanueva, Cintia Flores
Summary: A wide range of chemicals in drinking water and urine samples were analyzed through target and non-target screening to estimate human exposure. PFAS, bisphenol A, and nonylphenol were detected in tap water and urine samples, and LC-HRMS revealed the presence of pharmaceuticals and industrial chemicals. Reverse osmosis effectively removed PFAS, while activated carbon filters did not. These findings are important for exposure science and monitoring drinking water contaminants.
Article
Food Science & Technology
Monica Campas, Sandra Leonardo, Ainoa Ferre-Gode, Asif A. Chowdhury, Anna Toldra, Karl B. Andree, Ana Roque
Summary: Vibrio parahaemolyticus is a dominant cause of foodborne infections and can accumulate in shellfish. A duplex electrochemical biosensor was developed to detect the tdh and trh genes of V. parahaemolyticus in oysters, providing a quick and specific method for monitoring this pathogen.
Article
Biochemistry & Molecular Biology
Marylene Lejeune, Laia Reverte, Noelia Gallardo, Esther Sauras, Ramon Bosch, Daniel Mata, Albert Roso, Anna Petit, Vicente Peg, Francisco Riu, Joan Garcia-Fontgivell, Fernanda Relea, Begona Vieites, Luis de la Cruz-merino, Meritxell Arenas, Valeri Rodriguez, Juana Galera, Anna Korzynska, Benoit Plancoulaine, Tomas Alvaro, Carlos Lopez
Summary: This study characterized the residual tumor microenvironment in 96 TNBC patients and evaluated its prognostic implications for partial responders vs. non-responders. Partial responders showed higher levels of CD83+ mature dendritic cells, FOXP3+ regulatory T cells, and IL-15 expression but lower CD138+ cell concentration, leading to improved overall survival and recurrence-free survival. However, MMP-9 expression in the residual tumor microenvironment was identified as an independent factor associated with the impaired response to neoadjuvant chemotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Mounira Alkassar, Andres Sanchez-Henao, Jaume Reverte, Lourdes Barreiro, Maria Rambla-Alegre, Sandra Leonardo, Manolis Mandalakis, Panagiota Peristeraki, Jorge Diogene, Monica Campas
Summary: By analyzing the liver of a poisonous puffer fish caught in South Crete, Greece, researchers determined the toxicity equivalency factors (TEFs) of various tetrodotoxin (TTX) analogues. This study is crucial for toxin monitoring and food safety control.
