期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 43, 期 2, 页码 423-428出版社
AMER CHEMICAL SOC
DOI: 10.1021/es801325u
关键词
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资金
- European Commission [FP6-2004-Global-3.3.111.3.1]
- EIF Fellowship through the European Marie Curie Programme [CT2005-023699]
- [018391]
- Natural Environment Research Council [pml010003] Funding Source: researchfish
- NERC [pml010003] Funding Source: UKRI
Bacterial bioreporters have substantial potential for contaminant assessment, but their real world application is currently impaired by a lack of sensitivity. Here, we exploit the bioconcentration of chemicals in the urine of animals to facilitate pollutant detection. The shore crab Carcinus maenas was exposed to the organic contaminant 2-hydroxybiphenyl, and urine was screened using an Escherichia coli-based luciferase gene (luxAB) reporter assay specific to this compound. Bioassay measurements differentiated between the original contaminant and its metabolites, quantifying bioconcentration factors of up to one hundred-fold in crab urine. Our results reveal the substantial potential of using bacterial bioreporter assays in real-time monitoring of biological matrices to determine exposure histories, with wide ranging potential for the in situ measurement of xenobiotics in risk assessments and epidemiology.
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