期刊
ENVIRONMENTAL MONITORING AND ASSESSMENT
卷 187, 期 1, 页码 -出版社
SPRINGER
DOI: 10.1007/s10661-014-4147-1
关键词
Cytochrome P450 1A (CYP1A); Extracellular signal-regulated kinase (ERK); Heme oxygenase-1 (HO-1); Hypoxia-inducible factor-1 (HIF-1); Mugil cephalus; Stress
资金
- University Grants Commission, New Delhi, India [UGC: 41-1281/2012 (SR)]
Induction of heme oxygenase-1 (HO-1) and extracellular signal-regulated kinase (ERK) is a crucial step in the cellular response to oxidative stress. Altered expression of such proteins in response to stress conditions is a key factor for the maintenance of cellular integrity and survival. Hypoxia-inducible factor-1 (HIF-1) and cytochrome P450 1A (CYP1A) are critical indicators of environmental exposure to hypoxia. The current study was aimed to analyse the expression pattern of HO-1, p-ERK, HIF-1 alpha and CYP1A2 in hepatocytes of Mugil cephalus inhabiting Kovalam (unpolluted site) and Ennore (polluted site) estuaries. The impact of stress on hepatocytes was assessed by measuring the level of lipid hydroperoxides (LHP) and reduced glutathione (GSH) in hepatocytes of M. cephalus inhabiting these estuaries. The expression of HO-1, p-ERK, HIF1 alpha and CYP1A2 was analysed by ELISA and immunoblot analysis, respectively. There was significant decrease of GSH (19 %) and CYP1A2 (34 %) along with significant increase of LHP (31 %), HO-1 (96 %), p-ERK (p< 0.01) and HIF-1 alpha (41 %) in hepatocytes of M. cephalus inhabiting Ennore estuary than Kovalam estuary. The present study shows that increased synthesis of HO-1 mainly regulated by p-ERK and HIF-1 alpha may significantly contribute to the environmental adaptation processes of grey mullet, by protecting the cells from oxidative stress and stress-induced degenerative changes.
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