Article
Engineering, Chemical
Weijun Zhang, Jun Chen, Mingyue Tang, Hanjun Wu, Ming Liu, Jing Ai, Dongsheng Wang
Summary: The CA-Fenton process significantly improved sludge dewatering performance and removal of antibiotic resistance genes (ARGs). The acidification of CA enhanced permeability of the EPS cell barrier, releasing intracellular ARGs into the sludge and accelerating their degradation, while also improving sludge dewaterability by destroying the secondary structure of proteins. This study not only proposed an efficient method for traditional Fenton oxidation in sludge treatment, but also provided a mechanistic basis for understanding the CA-Fenton process and enabling the harmless disposal of sludge.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Hang Lv, Qiao Xiong, Daoguang Liu, Xu Wu
Summary: This study investigated the effects of electro-dewatering on subsequent low-temperature drying at various potentials and the characteristics of low-temperature air-drying sludge were explored through experiments and multi-physical modeling. Results showed that electro-dewatering reduced EPS content and changed the organic matter species, leading to a cake structure conducive to low-temperature drying. The applied higher electric field intensity in electro-dewatering stage promoted water vapor diffusion activity in air-drying stage, resulting in lower energy consumption and higher yield compared to directly low-temperature drying.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Ting Li, Jiawei Yang, Yujun Zhou, Xuan Liu, Yixin Luo, Di Fang, Jianru Liang, Jiansheng Li, Lixiang Zhou
Summary: In recent years, bioleaching has become a cost-effective technology for improving sludge dewaterability. This study developed a novel approach combining short-time bioleaching with polyferric sulfate flocculation, achieving comparable dewatering results to conventional long-time bioleaching. These findings provide new insights for developing efficient sludge dewatering strategies.
ENVIRONMENTAL RESEARCH
(2023)
Article
Engineering, Environmental
Ting Li, Jiawei Yang, Yujun Zhou, Yixin Luo, Bo Zhou, Di Fang, Jiansheng Li, Lixiang Zhou
Summary: Bioleaching facilitated by iron-oxidizing bacteria is a promising and cost-effective method for sludge dewatering. Increasing the production of reactive oxygen species (ROS) through the introduction of H2O2 can significantly improve dewaterability and shorten the treatment time.
Article
Engineering, Environmental
A. Angel Jessieleena, M. Priyanka, M. P. Saravanakumar
Summary: A comparative analysis of three assisted advanced oxidation processes was conducted to study their efficacy in enhancing tannery chemical sludge dewaterability. The Fe2+/(NH4)2S2O8 process was found to have the greatest impact on breaking down sludge cells and releasing chromium, organic carbon, protein, and total dissolved solids. The study also revealed a two-step mechanism of oxidation followed by coagulation in improving sludge dewaterability.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Jiamin Zhao, Tingting Hou, Zhongfang Lei, Kazuya Shimizu, Zhenya Zhang
Summary: A biogas recirculation-driven anaerobic digestion system was developed in this study, coupled with an alkali addition strategy, which not only improved biogas upgrading efficiency but also enhanced sludge settleability and dewaterability, reducing sludge management costs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Mostafa Ahmed, Gianni Andreottola, Sherien Elagroudy, Mohamed Shaaban Negm, Luca Fiori
Summary: The study found that increasing the hydrothermal carbonization (HTC) treatment time can improve the dewaterability of the treated slurry, but it also results in an increase in ammonium concentration in the HTC liquor (HTCL) and a decrease in methane potential. A treatment time of 1 hour was identified as the optimal trade-off for dewaterability and utilization of HTCL for biogas production.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Environmental Sciences
Jiajun Wang, Xiaoqing Meng, Yu Chen, Guanyu Zheng, Lixiang Zhou
Summary: The pre-acidification of sewage sludge combined with conditioning using Fenton's reagent can effectively attenuate antibiotic resistance genes (ARGs) and improve sludge dewaterability simultaneously. This process significantly reduces the absolute abundances of total ARGs and mobile genic elements (MGEs) in conditioned sludge, leading to better dewatering performance with reduced moisture content in the dewatered sludge cake.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Siru Zhou, Chuanhan Chen, Ruohong Li
Summary: This study optimized the Fe(II)/SPC process in a full-scale wastewater treatment plant using response surface methodology (RSM) and investigated its impact on sludge structure. The Fe(II)/SPC process showed higher economic efficiency and safety compared to other full-scale conditioning processes, while maintaining good dewatering performance.
Article
Chemistry, Analytical
Reza Rashvanlou, Hasan Pasalari, Abbas Ali Moserzadeh, Mahdi Farzadkia
Summary: Dewatering is a crucial step in sludge management, and using coagulant to condition the sludge is the most common way to improve its dewaterability. This study examined the sludge disintegration process using ultrasound and tested four coagulants. The results showed that ultrasound-assisted conditioning with cationic polyacrylamide (CPAM) and combined coagulant (lime + ferric chloride) significantly improved the sludge dewaterability.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Liyan Liu, Bin Ran, Chao Hua
Summary: The study demonstrated that the combined treatment of ultrasonic and KMnO4 is effective in enhancing the dewatering performance of drilling waste sludge, reducing water content and dewatering time, and thus reducing costs and secondary environmental pollution.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Environmental
Lianpeng Sun, Chuanhan Chen, Siru Zhou, Weifang Yuan, Hui Lu, Hao Wang, Xinzhe Zhu, Huanzhong Deng, Xiao-yan Li, Lin Lin, Ruo-hong Li
Summary: A novel iron-rich sludge conditioning method, called microbial iron reduction activating sodium percarbonate (MIR/SPC) treatment, was developed and optimized. The study found that MIR/SPC treatment significantly improved the sludge dewaterability. The optimal conditions for sludge conditioning were determined to be 5-day anaerobic MIR treatment, pH 3, and 1 g/L of SPC. Under these conditions, the conditioned sludge showed a reduction of over two-thirds in CST value and a decrease in moisture content to 51.5% in full-scale application. The critical mechanism of MIR-promoted advanced oxidation process (AOP) in sludge conditioning was explored.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Cristian B. Arenas, Ruben Gonzalez, Judith Gonzalez, Jorge Cara, George Papaharalabos, Xiomar Gomez, E. Judith Martinez
Summary: The study found that pre-treating waste activated sludge with electro-oxidation can significantly increase the soluble organic material content, improve hydrolysis rate, and methane yield. Additionally, applying electro-oxidation to improve digestate dewaterability can alter the protein and aliphatic matrix structure of the sample, resulting in the release of more water during drying.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Engineering, Chemical
Mingyue Tang, Zhiyue Wang, Weijun Zhang, Dongsheng Wang
Summary: Thermal activated persulfate oxidation was used to improve the dewaterability of wastewater sludge by decomposing macromolecular proteins. This process was found to generate beneficial radicals and enhance sludge dewaterability.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Jiaqi Hou, Yiming Li, Haixiao Guo, Yufen Wang, Yanying He, Tingting Zhu, Yiwen Liu
Summary: This study introduced the electro-Fenton technique by in-situ H2O2 generation into sludge dewatering for the first time, improving the dewatering performance. The electro-Fenton treatment reduced the surface negative charge of sludge and disrupted extracellular polymeric substances, enhancing flocculability and filtering performance. It also altered the secondary structure of proteins, resulting in a loose structure that benefited the exposure of hydrophobic sites or groups in EPS proteins.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Yile Hao, Qinhua Wang, Jie Li, Shihui Yang, Yanli Zheng, Wenfang Peng
Summary: This study reports a novel CRISPR-based genome editing technology established upon a Type I-F system. By utilizing nCas3 variants created by alanine-substituting catalytic residues, targeted double-strand breaks can be introduced, facilitating efficient genome editing without visible cell killing. This offers a simple approach to convert Type I systems into advanced genome editing tools.
Article
Chemistry, Multidisciplinary
Jun Yang, Fei Wang, Shihui Yang, Wenxue Jiang, Rong Gao, Siyu Lin, Binyang Deng, Xiaoyu Wang, Wei Cheng, Yang Liu, Chin-Yu Chen, Qiong Xing, Chao Zhai, Lixin Ma
Summary: This study identified protein pairs with ultrahigh affinity and used them to construct a hyperthermostable artificial scaffold for cellulose degradation. The complex exhibited a synergistic effect in hydrolyzing cellulosic substrates at elevated temperature.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Engineering, Biomedical
Junyan Wang, Wanqi Yu, Ruining Shi, Shihui Yang, Jingjie Zhang, Xiao Han, Zhe Zhou, Weijia Gao, Yongli Li, Jinghui Zhao
Summary: This study evaluates the effect of amino group modification on the osseointegration behavior of carbon fiber reinforced PEEK (30% CPEEK) implants in rabbit skulls. The results show that amino-functionalized implants have better bone-forming ability and osseointegration than non-modified implants.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Infectious Diseases
Binan Geng, Xingyu Huang, Yalun Wu, Qiaoning He, Shihui Yang
Summary: This study investigated antibiotic resistance genes in Z. mobilis using bioinformatic approaches and CRISPR/Cas12a-based genome-editing technology. Six ampicillin-resistant genes were identified, and mutant strains were constructed. The results showed that ZMO0103 played a key role in ampicillin resistance, and other ampicillin-resistant genes may have a synergetic effect with it. This study laid a foundation for further research on antibiotic resistance mechanisms.
Article
Multidisciplinary Sciences
Zhenzhen Liang, Zhouqing Luo, Weimin Zhang, Kang Yu, Hui Wang, Binan Geng, Qing Yang, Zuoyu Ni, Cheng Zeng, Yihui Zheng, Chunyuan Li, Shihui Yang, Yingxin Ma, Junbiao Dai
Summary: The relationship between gene sequence and function is important for both fundamental and practical reasons. This study systematically refactored yeast essential genes to explore the plasticity of gene sequences and identify the logic behind encoding and regulation. Further understanding of gene sequence choice can guide the design of genes in various applications.
Article
Chemistry, Multidisciplinary
Shangjie Zhang, Feng Guo, Qiao Yang, Yujia Jiang, Shihui Yang, Jiangfeng Ma, Fengxue Xin, Tomohisa Hasunuma, Akihiko Kondo, Wenming Zhang, Min Jiang
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was rationally engineered to utilize methanol and produce value-added chemicals like succinic acid. Through metabolic pathway engineering and gene regulation, the recombinant strain showed enhanced methanol utilization and cell growth. Transcriptome analysis revealed the rebalancing of methylotrophic metabolism. The study demonstrates the successful biological conversion of methanol into valuable chemicals.
Article
Biochemistry & Molecular Biology
Zhitao Mao, Qianqian Yuan, Haoran Li, Yue Zhang, Yuanyuan Huang, Chunhe Yang, Ruoyu Wang, Yongfu Yang, Yalun Wu, Shihui Yang, Xiaoping Liao, Hongwu Ma
Summary: CAVE is a cloud-based platform that integrates calculation, visualization, examination, and correction of metabolic pathways. It can analyze and visualize pathways for over 100 published genome-scale metabolic models or user-uploaded models. CAVE offers model modification functions for users to correct errors in pathway analysis and obtain more reliable pathways.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemical Research Methods
Shihui Yang, Ju Huang, Xia Wang, Xiangyu Chen, Han Li, Yunhao Chen, Zhousheng Hu
Summary: An evolved Zymomonas mobilis strain, F74, with improved molasses utilization was generated through adaptation in fructose, which is mainly attributed to a G99S mutation in Glf for enhanced fructose import. SacB deletion and sacC overexpression in F74 further enhanced the ethanol productivity by diverting sucrose metabolism from levan production to ethanol production. This strategy of adapting and engineering microbial strains for efficient molasses utilization provides a reference for developing microbial cell factories.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Microbiology
Weijia Gao, Xiao Han, Duo Sun, Yongli Li, Xiaoli Liu, Shihui Yang, Zhe Zhou, Yuanzheng Qi, Junjie Jiao, Jinghui Zhao
Summary: In this study, antimicrobial peptide HHC36 was fixed on the surface of sulfonated PEEK (SPEEK) through a simple solvent evaporation method, and the samples were characterized and evaluated for their antibacterial properties and cytocompatibility in vitro, as well as their anti-infection property and biocompatibility in vivo. The results showed that HSPEEK exhibited excellent antibacterial properties and good cell compatibility, reducing bacterial survival rate and inflammatory reaction in vivo.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ying Tang, Fei Wang, Yi Wang, Yuwei Wang, Yang Liu, Zhizhao Chen, Wenqiang Li, Shihui Yang, Lixin Ma
Summary: In this study, a prokaryotic Ago protein from a hyperthermophilic archaeon was characterized and found to have typical DNA-guided DNA endonuclease activity. The efficiency and accuracy of cleavage were regulated by various factors such as temperature, divalent ions, gDNA phosphorylation, and complementarity to DNA targets. It was also shown that TtdAgo had programmable cleavage activity towards double-stranded DNA and exhibited cell toxicity towards Zymomonas mobilis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lian-Ying Cao, Chen-Guang Liu, Shi-Hui Yang, Feng-Wu Bai
Summary: In this study, a novel strategy was developed to enhance the stress tolerance of Z. mobilis by regulating biofilm formation through heterologous expression of pfs and luxS genes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mimi Hu, Weiwei Bao, Qiqun Peng, Wei Hu, Xinyu Yang, Yan Xiang, Xiongying Yan, Mian Li, Ping Xu, Qiaoning He, Shihui Yang
Summary: In this study, a lactate producer of Z. mobilis was constructed by metabolic engineering and fermentation optimization. The engineered strain showed a high lactate production efficiency and a high total carbon conversion rate. It could efficiently produce lactate using waste feedstocks. This study demonstrated the potential of this lactate-producing strain as a biorefinery platform for carbon-neutral biochemical production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lingling Shangguan, Huiyan Zhang, Zixiong Liu, Feiran An, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Jun Dai, Xiong Chen
Summary: This study obtained a mutant strain, P-45, with improved glutamic acid production capacity and genetic stability through strain mutagenesis and screening. The glutamic acid concentration of P-45 under fed-batch fermentation conditions in 20 L tanks was increased by 81.5% compared to the starting strain.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Yalun Wu, Qianqian Yuan, Yongfu Yang, Defei Liu, Shihui Yang, Hongwu Ma
Summary: A high-quality genome-scale metabolic model (GEM) iZM516 of Zymomonas mobilis ZM4 was constructed, which combined improved genome annotation, literature, dataset of Biolog Phenotype Microarray studies, and updated Gene-Protein-Reaction information. It has the highest MEMOTE score among all published GEMs of Z. mobilis, and can predict cell growth and metabolic engineering strategies.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Weiwei Bao, Wei Shen, Qiqun Peng, Jun Du, Shihui Yang
Summary: Biorefinery for producing value-added biochemicals is a promising alternative for meeting sustainable energy and environmental goals. This study applied metabolic engineering strategies to enhance acetoin production in Zymomonas mobilis by introducing a heterologous acetoin pathway and manipulating NADH levels. The recombinant strain achieved significantly improved acetoin production.
FERMENTATION-BASEL
(2023)
