Article
Genetics & Heredity
Esther Blanco-Romero, David Duran, Daniel Garrido-Sanz, Rafael Rivilla, Marta Martin, Miguel Redondo-Nieto
Summary: Rhizosphere colonization by bacteria is regulated by transcription factors AmrZ and FleQ, which control gene expression related to motility, biofilm formation, nitrogen, sulfur, and amino acid metabolism. These findings contribute to a better understanding of the regulatory mechanisms involved in rhizosphere colonization.
MICROBIAL GENOMICS
(2022)
Article
Biotechnology & Applied Microbiology
Michela Maifreni, Giovanni Di Bonaventura, Marilena Marino, Simone Guarnieri, Francesca Frigo, Arianna Pompilio
Summary: This study aimed to determine the biofilm-forming ability of a strain belonging to the Pseudomonas fluorescens group isolated from the dairy environment under food-relevant conditions, and to assess the effects of commercial sanitizers against preformed biofilms. The results showed that the strain formed strong biofilms on polystyrene, regardless of temperature. The biofilms were mostly sensitive to chlorine and peracetic acid-based sanitizers. Different conditions of biofilm formation showed different tolerance to disinfectants. The findings from this study are significant for developing targeted sanitation protocols in food plants.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Agronomy
N. S. Nysanth, S. Divya, Chitra B. Nair, A. B. Anju, R. Praveena, K. N. Anith
Summary: Foot rot disease in black pepper caused by Phytophthora capsici is a serious issue as it can lead to plant mortality. Organic control methods using beneficial microorganisms such as Trichoderma spp. and Pseudomonas fluorescens show promise in managing this disease.
Article
Food Science & Technology
Francesca Maggio, Chiara Rossi, Clemencia Chaves-Lopez, Annalisa Serio, Luca Valbonetti, Francesco Pomilio, Alessio Pio Chiavaroli, Antonello Paparella
Summary: This study evaluated the interactions between Listeria monocytogenes and Pseudomonas fluorescens in biofilms simulating dairy processing conditions, as well as the capability of P. fluorescens to produce blue pigment. Results indicated that P. fluorescens could form biofilms on PS surfaces and in dual-species biofilms, L. monocytogenes cell population was higher on SS surfaces. Additionally, the production of blue pigment by P. fluorescens was observed in both single- and co-culture conditions.
Article
Microbiology
Zhongyou Li, Katja Koeppen, Victoria I. Holden, Samuel L. Neff, Liviu Cengher, Elora G. Demers, Dallas L. Mould, Bruce A. Stanton, Thomas H. Hampton
Summary: Researchers developed an algorithm called GAUGE, which automatically annotates GEO microbial data sets, increasing the percentage of analyzable data sets from 4% to 33%. The annotations provide valuable insights and facilitate the identification of consistent patterns of differential gene expression. Additionally, they created a web interface called GAPE for reanalyzing P. aeruginosa and E. coli transcriptomic data.
Article
Plant Sciences
Rafael Villar-Moreno, Sandra Tienda, Jose A. Gutierrez-Barranquero, Victor J. Carrion, Antonio de Vicente, Francisco M. Cazorla, Eva Arrebola
Summary: This study investigated the interactions between rhizospheric Pc strains and plant roots, focusing on their effects on root performance. The researchers found that PCL1606 has a better adaptation to nutrient-rich environments and forms biofilms. Additionally, PCL1601 and PCL1606 strains were well adapted to avocado roots and showed biocontrol activity against a fungal pathogen. These findings suggest that these strains could be used to construct a beneficial microbial community associated with avocado plant roots.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Ecology
I. C. Engelhardt, D. Patko, Y. Liu, M. Mimault, G. de las Heras Martinez, T. S. George, M. MacDonald, M. Ptashnyk, T. Sukhodub, N. R. Stanley-Wall, N. Holden, T. J. Daniell, L. X. Dupuy
Summary: This study used live microscopy techniques to observe collective movement of B. subtilis bacteria in soil, resembling the behavior of bird flocks or fish schools. Genetic analysis suggests that this movement may be driven by diffusion of extracellular signaling molecules and influenced by physical obstacles and hydrodynamics in the soil environment.
Article
Microbiology
Josue Jara, Francisco Alarcon, Ajay K. Monnappa, Jose Ignacio Santos, Valentino Bianco, Pin Nie, Massimo Pica Ciamarra, Angeles Canales, Luis Dinis, Ivan Lopez-Montero, Chantal Valeriani, Belen Orgaz
Summary: Under hydrodynamic stress, bacteria can adapt by increasing cell density and matrix production in biofilms. This adaptation leads to the formation of mechanically stable biofilms with a higher number of crosslinks and enriched carbohydrate compositions in the matrix, affecting both structure and viscoelastic properties.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Hidehiro Ishizawa, Masashi Kuroda, Daisuke Inoue, Michihiko Ike
Summary: Genome-wide transposon sequencing was used to identify genes involved in bacterial colonization and overall fitness on duckweed. The study found that the efficiency of initial colonization is mainly determined by the motility and cell surface structure of the bacteria, while overall fitness is associated with diverse metabolic and regulatory functions. Genes involved in the synthesis of lipopolysaccharides and type-IV pili showed different contributions to colonization and fitness, reflecting their metabolic cost and important roles in host association.
COMMUNICATIONS BIOLOGY
(2022)
Article
Ecology
Richa Sharma, Laurent Gal, Dominique Garmyn, David Bru, Shilpi Sharma, Pascal Piveteau
Summary: This study reveals that a bacterial consortium can significantly enhance plant growth and suppress the growth of pathogens. In sterilized soil, the inoculation of bioinoculants effectively inhibits the population of Listeria monocytogenes, but in non-sterile soil, the presence of resident soil bacterial communities also plays a crucial role in this inhibitory effect.
Article
Biotechnology & Applied Microbiology
Wendong Zhang, Xiaoyu Mi, Chen Zhang, Yu Cheng, Siqi Wang, Junke Ji, Yangyang Yuan, Longfeng Wang, Wenzheng Liu, Yun Jiang
Summary: This study examines the interplay patterns between Pseudomonas fragi and Escherichia coli in different conditions. The results show that competition is the major observed pattern of interaction between these two species. It is also found that prior colonization of one species significantly reduces the cell counts of the other species when competing for limited adhesion surface. Additionally, the spatial structure and microbial composition of mature dual-species biofilms are not significantly affected. Furthermore, the study reveals that E. coli and P. fragi enhance their biofilm formation capabilities by upregulating the expression level of specific genes.
Article
Biochemistry & Molecular Biology
Kurt M. Dahlstrom, Dianne K. Newman
Summary: Fungi are sensitive to toxic phenazines, but can be protected by certain bacterial partners such as Paraburkholderia edwinii, which sequesters the toxin and provides a reducing environment. This protective mechanism is widespread among Paraburkholderia and has implications for microbial communities in different environments.
Article
Plant Sciences
Priyanka Pathania, Divij Gulati, Hema Setia, Ranjana Bhatia
Summary: Two isolates TS4 and TS6 from tomato rhizosphere were found to possess different plant growth-promoting properties such as enzyme production and hydrogen sulfide (H2S) production. The colonization potential of TS4 and TS6 in the tomato rhizosphere was studied and it was observed that they had strong colonization ability. In greenhouse studies, these isolates showed positive influence on tomato growth. In summary, TS4 and TS6 are efficient plant growth-promoting rhizobacteria that can be used for high-yield cultivation in sustainable agriculture.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Ecology
Shifra Steinberg, Maor Grinberg, Michael Beitelman, Julianna Peixoto, Tomer Orevi, Nadav Kashtan
Summary: This study found that interactions between immigrant bacteria and resident microbiota affect the microscale spatial organization of leaf microbiota, especially due to the preferential attachment of microbiota cells near newly formed P. fluorescens aggregates.
Article
Microbiology
Antoine Zboralski, Hara Saadia, Amy Novinscak, Martin Filion
Summary: This study explored the role of different plant genes in the rhizosphere colonization ability of beneficial Pseudomonas spp. It was found that mutations in genes related to root architecture positively influenced colonization ability, while mutations in genes related to plant immunity had a negative impact.
Article
Genetics & Heredity
Daniel Garrido-Sanz, Paula Sansegundo-Lobato, Miguel Redondo-Nieto, Jachym Suman, Tomas Cajthaml, Esther Blanco-Romero, Marta Martin, Ondrej Uhlik, Rafael Rivilla
MICROBIAL GENOMICS
(2020)
Article
Microbiology
Daniel Garrido-Sanz, Miguel Redondo-Nieto, Marta Martin, Rafael Rivilla
Article
Environmental Sciences
M. Wang, D. Garrido-Sanz, P. Sansegundo-Lobato, M. Redondo-Nieto, R. Conlon, M. Martin, R. Mali, X. Liu, D. N. Dowling, R. Rivilla, K. J. Germaine
Summary: The soil microbiome is crucial for ecosystem services and can be affected by recalcitrant organic compounds in contaminated soils. Ecopiling is a bioremediation technique that involves biostimulation, bioaugmentation, and phytoremediation. Analysis of soil microbial communities during the Ecopiling process revealed diverse composition and key enzymes involved in hydrocarbon degradation. Leading bacterial genera were Alphaproteobacteria, while fungal community was mainly Ascomycota. Despite high abundance of Lysobacter, other genera showed potential for petrochemical degradation.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Maria Guirado, Daniel Garrido-Sanz, Oscar Pindado, Manuel Rodriguez-Rastrero, Luis Merino-Martin, Maria Jose Sierra, Olga Escolano, Rafael Rivilla, Rocio Millan
Summary: This study evaluated the efficiency of biochar combined with a bioaugmentation based on an autochthonous bacterial consortium in in-situ bioremediation, showing that this combination was significantly more effective than individual treatments in degrading diesel pollutants. The bioaugmentation-biochar combination led to over 20% diesel degradation in both pollution scenarios, accompanied by an increase in bacterial diversity, suggesting synergies for highly efficient and environmentally friendly bioremediation processes.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2021)
Article
Genetics & Heredity
Esther Blanco-Romero, David Duran, Daniel Garrido-Sanz, Rafael Rivilla, Marta Martin, Miguel Redondo-Nieto
Summary: Rhizosphere colonization by bacteria is regulated by transcription factors AmrZ and FleQ, which control gene expression related to motility, biofilm formation, nitrogen, sulfur, and amino acid metabolism. These findings contribute to a better understanding of the regulatory mechanisms involved in rhizosphere colonization.
MICROBIAL GENOMICS
(2022)
Article
Biotechnology & Applied Microbiology
Laura Barrientos-Moreno, Maria Antonia Molina-Henares, Maria Isabel Ramos-Gonzalez, Manuel Espinosa-Urgel
Summary: The second messenger c-di-GMP controls various processes in bacteria, and certain amino acids can modulate its levels. This study reveals that the transcriptional regulator ArgR connects arginine metabolism and c-di-GMP signaling in Pseudomonas putida, forming a complex balancing mechanism to control bacterial lifestyles.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Xi Jiang, Diana Coroian, Emma Barahona, Carlos Echavarri-Erasun, Rocio Castellanos-Rueda, Alvaro Eseverri, Jose A. Aznar-Moreno, Stefan Buren, Luis M. Rubio
Summary: Biological nitrogen fixation is the process of converting inert atmospheric nitrogen gas into reactive ammonia, which can reduce the dependency on nitrogen fertilizers in plants by introducing functional nitrogenase. NifB is a critical protein in nitrogen fixation and can be restored by expressing nifB genes from different sources. Accumulation of functional NifB proteins in chloroplasts and mitochondria is crucial for engineering biological nitrogen fixation in plants.
Article
Multidisciplinary Sciences
Esther Blanco-Romero, Daniel Garrido-Sanz, David Duran, Rafael Rivilla, Miguel Redondo-Nieto, Marta Martin
Summary: The AmrZ/FleQ hub plays a critical role in the regulation of environmental adaption in Pseudomonas ogarae F113, affecting motility, biofilm formation, and c-di-GMP turnover. AmrZ controls the expression of genes related to extracellular matrix production and its involvement in competitive rhizosphere colonization is independent of c-di-GMP.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Microbiology
Manuel Espinosa-Urgel
ENVIRONMENTAL MICROBIOLOGY
(2023)
Editorial Material
Microbiology
Rafael Rivilla, Jacob G. Malone
Review
Microbiology
Esther Blanco-Romero, David Duran, Daniel Garrido-Sanz, Miguel Redondo-Nieto, Marta Martin, Rafael Rivilla
Summary: Motility and biofilm formation are crucial traits in rhizosphere colonization by pseudomonads. The AmrZ-FleQ hub is a complex signaling network that regulates both traits. AmrZ and FleQ are general transcription factors that form a regulatory hub, inversely regulating traits such as motility, extracellular matrix production, and iron homeostasis.
Article
Genetics & Heredity
David Duran, David Vazquez-Arias, Esther Blanco-Romero, Daniel Garrido-Sanz, Miguel Redondo-Nieto, Rafael Rivilla, Marta Martin
Summary: The model rhizobacterium Pseudomonas ogarae F113 encodes three different Type VI secretion systems (T6SS) and an auxiliary module containing a gene encoding an orphan VgrG protein (VgrG5a). This cluster is only present in several subgroups of the P. fluorescens complex of species. The analysis of mutants affecting the vgrG5a and tfe8 genes has shown that the module is involved in bacterial killing, with Tfe8 identified as a bacterial killing effector and Tfi8 as its cognate immunity protein. Tfe8 effector destabilizes the membranes of target cells by the formation of pores.