Article
Plant Sciences
Xin-yu Li, Mei-lan Lin, Ping-ping Hu, Ning-wei Lai, Zeng-rong Huang, Li-song Chen
Summary: In this study, it was found that Cu toxicity significantly affected the growth and leaf function of citrus seedlings, with C. grandis demonstrating relatively higher Cu tolerance compared to C. sinensis.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Biochemistry & Molecular Biology
Fenglin Wu, Huiyu Huang, Mingyi Peng, Yinhua Lai, Qianqian Ren, Jiang Zhang, Zengrong Huang, Lintong Yang, Christopher Rensing, Lisong Chen
Summary: Copper toxicity affects the growth and gene expression of Citrus grandis, leading to reduced photosynthesis. However, it also triggers adaptive responses in the plant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Alissar Cheaib, Lamiaa M. Mahmoud, Christopher Vincent, Nabil Killiny, Manjul Dutt
Summary: This study investigates the impact of introducing Vitis vinifera mybA1 (VvmybA1) in 'Hamlin' sweet orange trees. Results show that the transgenic plants display intense purple color due to the accumulation of anthocyanins in leaf epidermal cells. Although there is a slight reduction in photosynthetic performance, other parameters remain unaffected.
Article
Engineering, Environmental
Huan-Huan Chen, Zhi-Chao Zheng, Wen-Shu Chen, Rong-Yu Rao, Xu-Feng Chen, Xin Ye, Jiuxin Guo, Lin -Tong Yang, Li -Song Chen
Summary: Boron (B) can alleviate Citrus copper (Cu)-toxicity, but the mechanism is not clear. This study investigated the effect of B on Cu-toxicity in citrus seedlings and found that Cu-stress increased root release of malate and total free amino acids (TFAA), contributing to Cu-tolerance. However, B addition did not further stimulate the secretion of malate and TFAA, and actually decreased Cu-stimulated secretion. B addition increased the concentrations of malate, citrate, and TFAA in Cu-toxic roots. Internal detoxification of Cu by low molecular weight compounds (LMWCs) played a role in B-mediated alleviation of Cu-toxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Yu Bin, Qi Zhang, Yue Su, Chunqing Wang, Qiqi Jiang, Zhen Song, Changyong Zhou
Summary: This study used RNA-seq to analyze the gene expression patterns of lemon plants in response to CYVCV infection. There were 3691 differentially expressed genes (DEGs) identified, which were components of different pathways involved in phenylpropanoid biosynthesis, brassinosteroid biosynthesis, flavonoid biosynthesis, and photosynthesis. The DEGs related to phytohormone metabolism and photosynthesis pathways were further enriched and analyzed. It was found that different phytohormone-related genes had different responses toward CYVCV infection, while almost all of the photosynthesis-related DEGs were down-regulated in the infected plants.
Article
Chemistry, Multidisciplinary
Ioannis E. Papadakis, Chrysovalantou Antonopoulou, Thomas Sotiropoulos, Christos Chatzissavvidis, Ioannis Therios
Summary: Magnesium is essential for the growth and development of plants, and its deficiency has become a concern for citrus orchards worldwide. This study investigated the effects of magnesium deficiency on various aspects of orange plants and emphasized the importance of magnesium management in citrus farming. It provides insights into increasing magnesium concentrations, understanding magnesium absorption mechanisms, and optimizing nutrient management in sweet orange trees.
APPLIED SCIENCES-BASEL
(2023)
Article
Forestry
Huaye Xiong, Haotian Ma, Huanyu Zhao, Linsheng Yang, Bin Hu, Jie Wang, Xiaojun Shi, Yueqiang Zhang, Heinz Rennenberg
Summary: In this study, the potential of nitrogen remobilization in citrus was quantified, and the interaction between photosynthesis, nitrogen metabolism, and carbohydrate metabolism with nitrogen remobilization was investigated using physiological, proteomic, and gene expression analyses. Citrus trees exposed to low nitrogen showed higher nitrogen remobilization efficiency compared to those exposed to high nitrogen. These findings have important implications for nitrogen fertilizer management in orchards.
Article
Horticulture
Yuzhen Tian, Ruiyi Fan, Jiwu Zeng
Summary: This study identified 18 JMJC domain-containing proteins from Citrus grandis and examined their expression patterns in the exocarps of three varieties of Huajuhong at different developmental stages. The proteins were unevenly distributed on six chromosomes and could be classified into five groups. Additionally, cis-acting elements like the abscisic acid-responsive element were found on the promoters of most of these proteins.
Article
Horticulture
Gaofeng Zhou, Xiaona Sun, Liping Zhang, Xiaoli Zeng, Guidong Liu, Ou Sheng
Summary: Boron (B) deficiency in citrus leads to the formation of corky split vein (CSV), likely due to the accumulation of lignin in the vascular bundle of the veins. The key enzymes and genes involved in lignin biosynthesis are upregulated under B deficiency stress, highlighting the essential role of lignin metabolism in CSV formation.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Narendra Singh, Radha Mohan Sharma, Anil Kumar Dubey, Om Prakash Awasthi, Ron Porat, Supradip Saha, Chellapilla Bharadwaj, Amitha Mithra Sevanthi, Amrender Kumar, Nimisha Sharma, Nir Carmi
Summary: The study identified the optimum harvesting stage for citrus fruits and found that titratable acidity and ascorbic acid reached their peak levels in the early harvesting stage, then decreased over time; while total soluble solids, TSS/TA ratio, and BrimA values showed an increasing trend in most hybrids. Juice content increased initially, stabilized, and then decreased. BrimA was found to be a better predictor of consumer acceptability compared to traditional maturity indexes, indicating the importance of specific values in determining the optimum harvest stage for citrus genotypes.
Article
Microbiology
Zhangyong Dong, Ishara S. Manawasinghe, Yinghua Huang, Yongxin Shu, Alan J. L. Phillips, Asha J. Dissanayake, Kevin D. Hyde, Meimei Xiang, Mei Luo
Summary: This study identified eleven Diaporthe species, including two new species, from cultivated C. grandis cv. Tomentosa in Huazhou, Guangdong Province, China. It is the first intensive study of endophytic Diaporthe species on C. grandis in China, providing valuable insights into the diversity and potential biocontrol agents of Diaporthe species. These findings will enhance current knowledge of Diaporthe species associated with C. grandis and aid in disease management strategies.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Sheng-yang Li, Christopher Vincent
Summary: This study found that water deficit affects the translocation dynamics of carbohydrate in citrus plants, delaying new flush growth. The presence of new flush altered the direction and speed of carbohydrate translocation.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Chemistry, Applied
An-An Zhang, Jia-Bao Ni, Alex Martynenko, Chang Chen, Xiao-Ming Fang, Chang-Jiang Ding, Jun Chen, Jian-Wei Zhang, Hong-Wei Xiao
Summary: Based on the concept of circular economy, this study investigated the electrohydrodynamic drying of citrus peel using alternating current (AC) or direct current (DC) sources at various voltage levels. The effects of EHD on drying characteristics, enzyme inactivation, and phytochemical contents were evaluated. The findings suggest that EHD drying with AC improves drying behaviors, inactivates enzymes, and retains the phytochemical properties of citrus peel.
Article
Agriculture, Multidisciplinary
Jiajing Chen, Gu Li, Haipeng Zhang, Ziyu Yuan, Wenyun Li, Zhaoxin Peng, Meiyan Shi, Wenyu Ding, Huixian Zhang, Yunjiang Cheng, Jia-Long Yao, Juan Xu
Summary: The study found a close correlation between the bitterness of citrus fruits and the presence of the 1,2RhaT gene, with pummelo identified as the genetic origin for FNH bitterness in modern citrus cultivars. Furthermore, certain transcription factor genes were shown to coexpress with genes related to FNH accumulation, potentially affecting the taste of citrus fruits.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Qian-Qian Ren, Zeng-Rong Huang, Wei-Lin Huang, Wei-Tao Huang, Huan-Huan Chen, Lin-Tong Yang, Xin Ye, Li-Song Chen
Summary: The study found that root adaptations to excess copper included increased biosynthesis and accumulation of secondary metabolites, enhanced copper retention in root cell walls, down- and upregulation of genes related to copper uptake and homeostasis, and improved capacity to maintain iron and energy homeostasis. While some genes and metabolites related to the detoxification of reactive oxygen and nitrogen species were elevated in roots exposed to excessive copper, they were unable to protect roots from oxidative damage.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Environmental Sciences
Bi-Sha Wu, Yin-Hua Lai, Ming-Yi Peng, Qian-Qian Ren, Ning-Wei Lai, Jincheng Wu, Zeng-Rong Huang, Lin-Tong Yang, Li -Song Chen
Summary: This study investigated the effects of pH-aluminum interactions on energy-rich compounds and phytohormones in sweet orange roots. The findings suggest that increased pH improves the adaptability of energy-rich compounds to aluminum toxicity and helps to maintain nutrient homeostasis and reduce oxidative stress and aluminum uptake.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Fei Lu, Pingping Hu, Meilan Lin, Xin Ye, Lisong Chen, Zengrong Huang
Summary: This study investigated the effects of excessive spraying of Bordeaux mixture (Bm) on the photosynthetic characteristics and chloroplast ultrastructure of citrus leaves. The results showed that Bm increased the leaf copper content, decreased the photosynthetic pigments content, and damaged the chloroplast ultrastructure, resulting in leaf chlorosis and photosynthetic inhibition. Citrus grandis exhibited lower copper absorption, higher light photon-electron transfer efficiency, relative integrity of chloroplast ultrastructure, and promoted antioxidant protection compared to Citrus sinensis, which contributed to its higher photosynthetic activity under excessive spraying of Bm.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Huan-Huan Chen, Xu-Feng Chen, Zhi-Chao Zheng, Wei-Lin Huang, Jiuxin Guo, Lin-Tong Yang, Li -Song Chen
Summary: Copper (Cu) excess is common in citrus orchards. This study investigated Cu-induced release of root exudates and its role in plant Cu-tolerance using sweet orange seedlings. The results showed that root-induced alkalization and Cu-stimulated release of root exudates played a key role in increasing root Cu accumulation and reducing Cu uptake and phytotoxicity.
Article
Biochemistry & Molecular Biology
Jiang Zhang, Xu-Feng Chen, Wei-Lin Huang, Huan-Huan Chen, Zeng-Rong Huang, Xin Ye, Li-Song Chen
Summary: The study showed that high pH can alleviate oxidative damage caused by copper toxicity in plants by reducing the generation of reactive oxygen species (ROS) and methylglyoxal (MG), and coordinating detoxification systems. In contrast, low pH disrupts the balance between generation and removal, leading to oxidative damage in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Horticulture
Huanhuan Chen, Wenlang Hu, Yuwen Wang, Ping Zhang, Yi Zhou, Lin-Tong Yang, Yan Li, Li-Song Chen, Jiuxin Guo
Summary: Ammonium supply inhibits the growth of citrus seedlings, reduces photosynthetic nitrogen use efficiency, damages the photosynthetic electron transport chain, and increases reactive oxygen metabolism, with varying severity at different nitrogen levels.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Xin-Yu Li, Mei-Lan Lin, Fei Lu, Xin Zhou, Xing Xiong, Li-Song Chen, Zeng-Rong Huang
Summary: Excessive copper-based agrochemicals are toxic to citrus trees, resulting in starch granules accumulation in the leaves and roots. C. grandis has a higher copper tolerance than C. sinensis.
Article
Plant Sciences
Jiang Zhang, Wei-Lin Huang, Wei-Tao Huang, Xu-Feng Chen, Huan-Huan Chen, Xin Ye, Lin-Tong Yang, Li-Song Chen
Summary: The effects of copper-pH interactions on HRMs levels in Citrus sinensis leaves and roots were investigated. Increased pH mitigated Cu toxicity-induced alterations of HRMs, while Cu toxicity increased low-pH-induced alterations of HRMs. The maintenance of hormone homeostasis in Cu-treated roots and leaves suggested improved growth, while the accumulation of stress-related hormones in Cu-treated leaves and roots might inhibit growth.
Article
Engineering, Environmental
Huan-Huan Chen, Zhi-Chao Zheng, Wen-Shu Chen, Rong-Yu Rao, Xu-Feng Chen, Xin Ye, Jiuxin Guo, Lin -Tong Yang, Li -Song Chen
Summary: Boron (B) can alleviate Citrus copper (Cu)-toxicity, but the mechanism is not clear. This study investigated the effect of B on Cu-toxicity in citrus seedlings and found that Cu-stress increased root release of malate and total free amino acids (TFAA), contributing to Cu-tolerance. However, B addition did not further stimulate the secretion of malate and TFAA, and actually decreased Cu-stimulated secretion. B addition increased the concentrations of malate, citrate, and TFAA in Cu-toxic roots. Internal detoxification of Cu by low molecular weight compounds (LMWCs) played a role in B-mediated alleviation of Cu-toxicity.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Plant Sciences
Jiuxin Guo, Yiling Jiao, Yuwen Wang, Wenlang Hu, Yamin Jia, Zengrong Huang, Li -Song Chen
Summary: The study provides insights into the effects of Mg supply on Mg and Ca homeostasis in citrus plants, including the slowing down of root and shoot growth under Mg deficiency and the changes in concentrations and distribution of Mg and Ca in different organs.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Horticulture
Ming-Yi Peng, Qian-Qian Ren, Yin-Hua Lai, Jiang Zhang, Huan-Huan Chen, Jiuxin Guo, Lin-Tong Yang, Li-Song Chen
Summary: The contribution of nitrogen-deficiency-responsive metabolites and genes to long-term nitrogen-deficiency tolerance in Citrus, specifically sweet orange seedlings, is still poorly understood. Through studying the metabolites and genes that respond to long-term nitrogen-deficiency, as well as the impacts on physiological parameters in leaves, it was found that extensive gene and metabolite reprogramming occurred. These findings provide new information on the adaptive strategies of leaves to long-term nitrogen-deficiency.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Yin-Hua Lai, Ming-Yi Peng, Rong-Yu Rao, Wen-Shu Chen, Wei-Tao Huang, Xin Ye, Lin-Tong Yang, Li-Song Chen
Summary: Extensive metabolic and gene reprogramming occurred in Citrus sinensis seedlings under nitrogen deficiency, enhancing the ability to maintain phosphate and nitrogen homeostasis, improving energy production and utilization, enhancing metabolite transport, and activating protein processing. Additionally, the roots under nitrogen deficiency exhibited higher antioxidant capacity and delayed senescence.
Article
Horticulture
Bi-Sha Wu, Wei-Tao Huang, Rong-Yu Rao, Wen-Shu Chen, Dan Hua, Ning-Wei Lai, Jincheng Wu, Lin-Tong Yang, Li-Song Chen
Summary: This study reveals the contribution of aluminum-pH interaction to aluminum tolerance in Citrus leaves. Elevated pH can alleviate aluminum toxicity and enhance the adaptability of metabolites and gene expression to aluminum stress.
SCIENTIA HORTICULTURAE
(2023)
Article
Horticulture
Dan Hua, Wen-Shu Chen, Rong-Yu Rao, Xu-Feng Chen, Huan-Huan Chen, Ning-Wei Lai, Lin-Tong Yang, Xin Ye, Li -Song Chen
Summary: This study revealed that nitrogen deficiency can lead to increased production of reactive oxygen species (ROS) and methylglyoxal (MG), but also enhances the ability of leaves and roots to detoxify these molecules. The effects of nitrogen deficiency on ROS and MG generation and their detoxification systems were found to be different in leaves and roots, with roots being more affected.
SCIENTIA HORTICULTURAE
(2024)
Article
Forestry
Hao Xu, Ziwei Luo, Wenlang Hu, Yamin Jia, Yuwen Wang, Xin Ye, Yan Li, Li-Song Chen, Jiuxin Guo
Summary: Magnesium is an essential nutrient for plant growth and development. This study reveals the effects of magnesium deficiency on citrus plants, including decreased biomass and changes in magnesium concentration and distribution in different organs. Magnesium exists mainly in inorganic and water-soluble forms, and its mobility is related to subcellular distribution and chemical forms. Magnesium deficiency improves magnesium transport from parent to branch organs.
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)