期刊
JOURNAL OF PROTEOMICS
卷 124, 期 -, 页码 79-87出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2015.03.037
关键词
Obesity; Omental adipose tissue; Mitochondrial proteome; Adipocytes
资金
- European Society of Pediatric Endocrinology (ESPE)
- European Molecular Biology Organization (EMBO)
- Medical Research Council [MC_U105663150, MC_U105663148] Funding Source: researchfish
- MRC [MC_U105663148, MC_U105663150] Funding Source: UKRI
Impaired mitochondrial function is important in obesity and the development of insulin resistance and diabetes. The aim of this study was to identify human adipocyte-derived mitochondrial proteins associated with obesity. Mitochondrial proteins from 20 abdominal omental adipose tissue biopsies (13 obese and 7 control subjects) were separated by anion-exchange chromatography coupled to SDS-PAGE. Protein contents were compared and identified by MALDI-TOF-TOF mass spectrometry. Proteins of interest were validated, verified and quantified using immuno dot blot assays in a total of 76 mitochondrial preparations from both obese and non-obese patients. Mass spectrometric comparison of 20 mitochondrial proteomes yielded 62 proteins that were differentially expressed in adipose tissue of obese subjects. The immunological quantification of 12 mitochondrial proteins from 76 omental adipose tissue biopsies revealed four proteins, citrate synthase, HADHA, LETM1 and mitofilin inversely being associated with BMI, and mitofilin being inversely correlated with gender. Biological significance The finding that obese human subjects have reduced levels of important mitochondrial proteins in adipocytes of omental adipose tissue as compared to non-obese controls gives new insights in the impairment of mitochondrial function in this specialized compartment of human adipose tissue in obesity and may eventually lead to the definition of valuable obesity markers. (C) 2015 Elsevier B.V. All rights reserved.
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