期刊
JOURNAL OF PROTEOME RESEARCH
卷 14, 期 9, 页码 4039-4049出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.5b00576
关键词
strontium-90; internal emitter; radiation exposure; lipidomics; UPLC; data-independent mass spectrometry; phospholipid; triacylglycerol
资金
- National Institute of Health (National Institute of Allergy and Infectious Diseases) [U19 A1067773]
- Proteomic and Metabolomics Shared Resources, NIH [P30 CA51008]
Despite considerable research into the environmental risks and biological effects of exposure to external beam gamma rays, incorporation of radionuclides has largely been understudied. This dosimetry and exposure risk assessment is challenging for first responders in the field during a nuclear or radiological event. Therefore, we have developed a workflow for assessing injury responses in easily obtainable biofluids, such as urine and serum, as the result of exposure to internal emitters cesium-137 (Cs-137) and strontium-90 (Sr-90) in mice. Here we report on the results of the untargeted lipidomic profiling of serum from mice exposed to 90Sr. We also compared these results to those from previously published Cs-137 exposure to determine any differences in cellular responses based on exposure type. The results of this study conclude that there is a gross increase in the serum abundance of triacylglycerides and cholesterol esters, while phostaphatidylcholines and lysophosphatidylcholines displayed decreases in their serum levels postexposure at study days 4, 7, 9, 25, and 30, with corresponding average cumulative skeleton doses ranging from 1.2 +/- 0.1 to 5.2 +/- 0.73 Gy. The results show significant perturbations in serum lipidome as early as 2 days postexposure persisting until the end of the study (day 30).
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