Article
Agricultural Engineering
Sameh Samir Ali, Haixin Jiao, Shimaa El-Sapagh, Jianzhong Sun
Summary: This study developed a cellulase-producing bacterial consortium from wood-feeding termites that effectively degraded willow sawdust and increased methane production. The CBC consortium exhibited positive effects on cellulose bioconversion and accelerated the degradation of willow sawdust. The findings will contribute to the development of cellulolytic bacterial consortia for wood pretreatment in lignocellulosic anaerobic digestion biorefineries.
BIORESOURCE TECHNOLOGY
(2023)
Review
Chemistry, Physical
Goldy Shah, Ejaz Ahmad, K. K. Pant, V. K. Vijay
Summary: Swing adsorption technologies are considered as a flexible, highly automated, low energy consumption, and low capital investment purification technology for biogas. This review examines the impact of various factors on the efficiency of biogas purification using swing adsorption technologies.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Agricultural Engineering
Ali H. Bahkali, Asad Syed, Abdallah M. Elgorban, Mohamed A. Abdel-Wahab, Neha Srivastava, Vijai Kumar Gupta
Summary: This study reports the preparation of a nanocatalyst using date seed and evaluates its impact on cellulolytic enzyme production. The nanocatalyst significantly improves the production of the enzyme, with 30 IU/gds filter paper activity achieved under optimized conditions. In addition, the nanocatalyst enhances glucose production through the hydrolysis of acidic-pretreated date seeds, and promotes biohydrogen production through cocultured dark fermentation.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Donglin Xin, Benedikt M. Blossom, Xiaoyun Lu, Claus Felby
Summary: This study evaluated the effects of ascorbic acid and gallic acid on cellulase hydrolytic action and found that ascorbic acid increased the hydrolysis of cellulose by cellulases, while gallic acid had no effect. The effect of ascorbic acid differed for different types of cellulase enzymes.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Dan Li, Kang Li, Yanzehua Liu, Liping Wang, Na Liu, Shaomeng Huang
Summary: A mixed bacterial community (MBC) with excellent biodegradation ability of PAHs was developed from the activated sludge of a coking wastewater plant. The degradation rates of phenanthrene and pyrene by MBC were over 99% after 5 days. The dominant bacteria in MBC were affected by intermediate metabolites.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Agricultural Engineering
Meena Sankar, Reshma M. Mathew, Anoop Puthiyamadam, Athiraraj Sreeja-Raju, Meera Christopher, Digambar Vitthal Gokhale, Rajeev K. Sukumaran
Summary: Secretome analyses showed that the types of proteins secreted by SSF and SmF enzymes were similar, but SSF had high abundance of specific beta glucosidases, lytic polysaccharide monooxygenases and hemicellulolytic enzymes, resulting in efficient initiation, low cellobiose accumulation and high initial reaction rates.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Cuiyi Liang, Qiong Wang, Wen Wang, Carol Sze Ki Lin, Yunzi Hu, Wei Qi
Summary: This study reports an efficient cellulase cocktail produced by co-cultivation of several Penicillium strains with lytic polysaccharide monooxygenases (LPMOs) producing strains. When blending the consortium-derived enzyme cocktail with commercial cellulase preparation, the highest hydrolysis yield reached 88.7%, indicating its potential for enhancing lignocellulose biodegradation.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agricultural Engineering
Debasree Sinha, Sandipan Banerjee, Subhrangshu Mandal, Aman Basu, Aishiki Banerjee, Srinivasan Balachandran, Narayan Chandra Mandal, Shibani Chaudhury
Summary: The study isolated cellulolytic bacteria from termite-gut and soil, identifying them as Microbacterium sp. and Arthrobacter sp. These bacteria showed significant cellulase activity and could enhance biogas production. Bioaugmentation with these bacteria improved biogas production from Lantana camara biomass substrate.
BIORESOURCE TECHNOLOGY
(2021)
Article
Ecology
Ling-Dong Shi, Pan-Long Lv, Simon J. McIlroy, Zhen Wang, Xiao-Li Dong, Angela Kouris, Chun-Yu Lai, Gene W. Tyson, Marc Strous, He-Ping Zhao
Summary: This study provides evidence of interspecies electron transfer between bacterial populations in a community where selenate reduction is linked to methane oxidation. Methylocystis is identified as the most abundant methanotroph, while Pseudoxanthomonas, Piscinibacter, and Rhodocyclaceae populations are responsible for selenate reduction.
Article
Oncology
Anna Grenda, Ewelina Iwan, Pawel Krawczyk, Malgorzata Frak, Izabela Chmielewska, Arkadiusz Bomba, Aleksandra Giza, Anna Rolska-Kopinska, Michal Szczyrek, Robert Kieszko, Tomasz Kucharczyk, Bozena Jarosz, Dariusz Wasyl, Janusz Milanowski
Summary: The composition of gut microbiota may affect the effectiveness of immunotherapy and the survival period of patients with non-small cell lung cancer.
Article
Agriculture, Multidisciplinary
Weixin Zhang, Junqi Guo, Xiaoxiao Wu, Yajing Ren, Chunyan Li, Xiangfeng Meng, Weifeng Liu
Summary: The engineered T. reesei strain showed significant differences in secreted protein composition and enzymatic activity compared to the wild-type strain, demonstrating great potential for improving saccharification efficiency of hemicellulosic constituents within corn fiber.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Engineering, Environmental
Ruomu Chen, Junwei Huang, Xiaomeng Li, Chen Yang, Xiangwei Wu
Summary: In this study, an IBU-mineralizing consortium C6 was obtained and two IBU degraders, Streptomyces sp. D218 and Pseudomonas sp. M20, were isolated. The results suggest that the degradation of IBU in the consortia C1 and C6 may be mainly mediated by key players of Streptomyces and Pseudomonas, respectively.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Touijer Hanane, Benchemsi Najoua, Hamdi Salsabil, Janati Idrissi Abdellatif, Bousta Dalila, Irfan Ahmad, Sayyad Ali Raza BukharI, Muhammad Irfan, Lijing Chen, Bekkari Hicham
Summary: This study found that yeast strains isolated from the dung of ruminants and the gut of coprophagous insects can produce cellulases and other hydrolytic enzymes. They are also able to utilize various carbon sources and tolerate high concentrations of glucose, salt, and ethanol.
FERMENTATION-BASEL
(2022)
Article
Agricultural Engineering
Esteban Orellana, Leandro D. Guerrero, Carol Davies-Sala, Melisa Altina, Rodrigo M. Pontiggia, Leonardo Erijman
Summary: This study used gene and genome-centric metagenomics to examine microbiome dynamics in anaerobic digesters. The results showed that changes in feedstock composition can establish new microbial niches for bacteria with the capacity to degrade newly added substrates. Food waste concentration correlated with extracellular enzymes and metagenome-assembled genomes (MAGs) involved in the degradation of complex carbohydrates commonly found in fruits and plant cell walls.
BIORESOURCE TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Ran Du, Chong Li, Weichao Lin, Carol Sze Ki Lin, Jianbin Yan
Summary: This study constructed an artificial consortium with improved biomass conversion capability and good operation stability for consolidated bioprocessing (CBP) in biomass biorefinery. The diversity of microbial community structure and gene functions of the domesticated consortium was analyzed, showing a simplified microbial structure and aggregation of functional genes related to conversion of cellulosic materials for biofuel production. CBP of wheat straw using the domesticated consortium achieved the highest yield ever reported for ethanol and solvent production. These results highlight the potential of the domesticated consortium in lignocellulosic biomass biorefinery.
Article
Microbiology
Regina Rettenmaier, Wolfgang Liebl, Vladimir V. Zverlov
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2020)
Article
Microbiology
Regina Rettenmaier, Martina Schneider, Bernhard Munk, Michael Lebuhn, Sebastian Juenemann, Alexander Sczyrba, Irena Maus, Vladimir Zverlov, Wolfgang Liebl
Article
Biotechnology & Applied Microbiology
Nils Thieme, Johanna C. Panitz, Claudia Held, Birgit Lewandowski, Wolfgang H. Schwarz, Wolfgang Liebl, Vladimir Zverlov
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Microbiology
Regina Rettenmaier, Yat Kei Lo, Larissa Schmidt, Bernhard Munk, Ilias Lagkouvardos, Klaus Neuhaus, Wolfgang Schwarz, Wolfgang Liebl, Vladimir Zverlov
Article
Biotechnology & Applied Microbiology
Oksana V. Berezina, Sergey V. Rykov, Angelina K. Polyakova, Marine E. Bozdaganyan, Anna V. Sidochenko, Melanie Baudrexl, Wolfgang H. Schwarz, Vladimir V. Zverlov, Sergey V. Yarotsky
Summary: By mutating the thermostable endo-processive xyloglucanase MtXgh74, the influence of aromatic amino acids on the enzyme's mode of action and ability to reduce xyloglucan viscosity was investigated. Mutants Mut I and Mut II showed different K-m values and modes of action, with Mut II demonstrating superior reduction of xyloglucan viscosity. Mutant Mut III performed similarly to the wild-type enzyme MtXgh74, but lost the ability to cleave xyloglucan building blocks.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Asli Isci, Nils Thieme, Anne Lamp, Vladimir Zverlov, Martin Kaltschmitt
Summary: This study investigated the effect of microwave assisted deep eutectic solvent pretreatment on the solubilization of xylan and release of xylo-oligosaccharides from wheat straw. It was found that the increase in microwave energy led to linear decrease in xylan yield in the solid fraction, while the xylose monomer and oligosaccharide yields in the liquid fraction initially increased and then declined. Addition of water in deep eutectic solvent resulted in approximately 68% reduction in furfural yield.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Microbiology
Yajing Liu, Sonja Vanderhaeghen, Werner Feiler, Angel Angelov, Melanie Baudrexl, Vladimir Zverlov, Wolfgang Liebl
Summary: Arabinofuranosidases are important enzymes in the degradation of arabinose-containing polysaccharides. Two arabinofuranosidases, AmAraf51 and AmAraf43, from Acetivibrio mesophilus showed different substrate preferences and optimal working temperatures. AmAraf51 displayed exo-beta-xylosidase side activity and high thermostability, making it a promising candidate for industrial applications.
Article
Biotechnology & Applied Microbiology
Sergey Rykov, Alina Selimzyanova, Alena Y. Nikolaeva, Vladimir A. Lazarenko, Nikita Tsurin, Philipp Akentyev, Vladimir V. Zverlov, Wolfgang Liebl, Wolfgang H. Schwarz, Oksana Berezina
Summary: In this study, the properties and structures of three fungal GH12 enzymes were compared. It was found that the Egh12 endoglucanase and Bgh12A endoglucanase both had activity on linear beta-glucan, while the Xgh12B xyloglucanase did not. The importance of the endoglucanase-specific loop 1 in correct placement of substrates was demonstrated through comparative analysis and docking studies. These findings provide potential targets for improving enzymatic properties.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Regina Rettenmaier, Nils Thieme, Johanna Streubel, Luca Di Bello, Marie-Louise Kowollik, Liren Huang, Irena Maus, Andreas Klingl, Wolfgang Liebl, Vladimir V. Zverlov
Summary: Strain MD1(T) is an anaerobic, Gram-stain-negative bacterium isolated from a lab-scale biogas fermenter fed with maize silage. It displays unique morphological, metabolic, and genomic characteristics, suggesting its potential important role in the biogas fermentation process.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Nils Thieme, Wolfgang Liebl, Vladimir V. Zverlov
Summary: Clostridium beijerinckii strain mbf-VZ-132, isolated from soil in Bavaria, Germany, showed a high sequence identity with Clostridium diolis DSM 15410. The draft genome sequence of C. beijerinckii mbf-VZ-132 was obtained using PacBio sequencing in this study.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Nils Thieme, Regina Rettenmaier, Wolfgang Liebl, Vladimir V. Zverlov
Summary: In this study, the draft genome sequence of Mobilitalea sibirica strain P3M-3(T) was presented based on Illumina paired-end sequencing. This strictly anaerobic, halotolerant, organotrophic bacterium of the family Lachnospiraceae can utilize various plant-derived polysaccharides as its carbon source. The strain was originally isolated from a microbial mat in western Siberia (Russia).
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Simon Heinze, Ilias Lagkouvardos, Wolfgang Liebl, Wolfgang H. Schwarz, Petra Kornberger, Vladimir V. Zverlov
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)