Article
Microbiology
Alessandra Carattoli, Gabriele Arcari, Giulia Bibbolino, Federica Sacco, Dario Tomolillo, Federica Maria Di Lella, Maria Trancassini, Luigi Faino, Mario Venditti, Guido Antonelli, Giammarco Raponi
Summary: A study conducted in a university hospital in Rome, Italy identified multiple CZA-resistant KPC-3 protein variants, including novel variants that had not been reported before. These variants showed different sensitivities and activities to antibiotics, possibly due to mutations caused by amino acid substitutions, insertions, and deletions.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Lin Huang, Min Cao, Yanyan Hu, Rong Zhang, Yufei Xiao, Gongxiang Chen
Summary: The trend of fosfomycin resistance among KPC-KP strains is increasing in China, with the two major mechanisms of resistance being plasmid-mediated fosfomycin resistance gene fosA3 and mutation of the target gene glpT.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Microbiology
Jacqueline Findlay, Laurent Poirel, Mario Juhas, Patrice Nordmann
Summary: This study evaluated the impact of KPC variants on CZA resistance, finding increased susceptibility to cephalosporins and carbapenems but increased resistance to ceftazidime and piperacillin-tazobactam. The KPC variants exhibited increased affinity towards ceftazidime and slightly decreased sensitivity to avibactam, impacting CZA resistance while concurrently negatively impacting carbapenemase activities.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Federica Bovo, Tiziana Lazzarotto, Simone Ambretti, Paolo Gaibani
Summary: The aim of this study was to compare the BMD method with the DD test and strip test in assessing the antimicrobial activity of CFD against KPC-Kp strains. The CA between the BMD method and DD test was 92%, while the CA between the BMD method and strip test was 90.7%. KPC-Kp strains showing resistance to CFD were 36% by three methods. The DD test was found to be a valid alternative to the BMD method, while the strip test exhibited major limitations.
Article
Infectious Diseases
Constantinos Tsioutis, Vanessa M. Eichel, Nico T. Mutters
Summary: This critical review aimed to evaluate the effectiveness of measures to control the spread of KPC-KP in healthcare settings, finding that successful control during outbreaks doesn't necessarily indicate the effectiveness of individual infection control measures, due to low-quality evidence and lack of controlled intervention studies. The distribution of studies, reported cases, and high mortality rates highlight that KPC-KP remains a major healthcare problem globally.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Carolina Sabenca, Eliana Costa, Sara Sousa, Lillian Barros, Ana Oliveira, Sonia Ramos, Gilberto Igrejas, Carmen Torres, Patricia Poeta
Summary: The emergence of Klebsiella pneumoniae strains producing ESBL and KPC has become a major public health concern. The ability of these strains to form biofilms is worrisome as it can facilitate the spread of antibiotic resistance and prolong infections in healthcare facilities.
Article
Infectious Diseases
Eva Gato, Paula Guijarro-Sanchez, Isaac Alonso-Garcia, Rosa Pedraza-Merino, Adrian Conde, Emilio Lence, Soraya Rumbo-Feal, Andrea Pena-Escolano, Cristina Lasarte-Monterrubio, Tania Blanco-Martin, Ana Fernandez-Gonzalez, M. del Carmen Fernandez-Lopez, Romina Maceiras, Marta Martinez-Guitian, Juan Carlos Vazquez-Ucha, Luis Martinez-Martinez, Concepcion Gonzalez-Bello, Jorge Arca-Suarez, Alejandro Beceiro, German Bou
Summary: By studying high-risk Klebsiella pneumoniae strains, we found that imipenem/relebactam was more effective than ceftazidime/avibactam in preventing resistance mutations. In some strains, imipenem/relebactam resistance developed faster and reached higher levels compared to ceftazidime/avibactam. Lineages that evolved in the presence of ceftazidime/avibactam showed KPC substitutions associated with high-level resistance, while lineages that evolved in the presence of imipenem/relebactam showed specific substitutions related to imipenem/relebactam resistance.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Jee Hong Kim, Yun Young Cho, Ji Young Choi, Yu Mi Wi, Kwan Soo Ko
Summary: This study investigated the characteristics of KPC-2-producing Klebsiella pneumoniae isolates from a hospital in South Korea. It identified two main clones, ST11 and ST307, with different resistance profiles and virulence levels. Continuous monitoring of these clones, which show different characteristics including resistance and virulence, is necessary.
Article
Microbiology
Qiwei Chen, Lizhang Liu, Xiaofang Hu, Xu Jia, Xiaowei Gong, Youjun Feng, Man Huang
Summary: The study focuses on the diversity of KPC-2-producing plasmids in highly transmissible ST11 clones of carbapenem-resistant K. pneumoniae (CRKP). The researchers report a clinical case of a rare ST437 CRKP isolate, K186, which produces KPC-2. Whole-genome sequencing revealed three distinct resistance plasmids, one of which, pK186_KPC, is a small IncN-type plasmid of 26,012bp. The study further suggests that the carbapenem-resistant pK186_KPC might have arisen from the integration of ancestral IncN and IncFII plasmids.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Lina Zhao, Xinxin Xia, Ting Yuan, Junying Zhu, Zhen Shen, Min Li
Summary: This study analyzed genomic data of carbapenemase-carrying Klebsiella pneumoniae (KPN) strains in China, revealing rich diversity in sequence types and capsular serotypes. The study also found that carbapenemase has spread to hypervirulent KPN, mammals, poultry, and insects. The prevalence of different capsular serotypes has changed over time, with ST11 replacing KL47 as the dominant serotype. Different genic indicators can be used to differentiate between types of KPN.
Article
Microbiology
Agnes B. Jousset, Saoussen Oueslati, Cecile Emeraud, Remy A. Bonnin, Laurent Dortet, Bogdan I. Iorga, Thierry Naas
Summary: Resistance to the combination of ceftazidime (CAZ) and avibactam (AVI) is increasing, with a CAZ-AVI-resistant Klebsiella pneumoniae strain reported here. This strain, belonging to the high-risk sequence type 307 (ST307) clone, produces Klebsiella pneumoniae carbapenemase 39 (KPC-39), a single-amino-acid variant of KPC3. The study found that KPC-39 has lost carbapenemase activity but shows increased affinity for CAZ, suggesting potential silent dissemination in European healthcare settings.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
Theodoros Karampatakis, Charalampos Zarras, Styliani Pappa, Eleni Vagdatli, Elias Iosifidis, Emmanuel Roilides, Anna Papa
Summary: This study genetically characterized four ST39 CRKP isolates simultaneously producing VIM-1 and KPC-2, obtained in a Greek tertiary hospital. The isolates were pandrug-resistant or extensively drug-resistant, and carried multiple antimicrobial resistance genes and plasmids. The analysis revealed a close clonal relatedness among the isolates.
MICROBIAL PATHOGENESIS
(2022)
Article
Biology
Natalia C. Rosas, Jonathan Wilksch, Jake Barber, Jiahui Li, Yanan Wang, Zhewei Sun, Andrea Rocker, Chaille T. Webb, Laura Perlaza-Jimenez, Christopher J. Stubenrauch, Vijaykrishna Dhanasekaran, Jiangning Song, George Taiaroa, Mark Davies, Richard A. Strugnell, Qiyu Bao, Tieli Zhou, Michael J. McDonald, Trevor Lithgow
Summary: This study reconstructed the genetic and evolutionary mechanisms of carbapenem resistance in a clinical isolate of Klebsiella quasipneumoniae. It found that multiple genetic loci are necessary for acquiring carbapenem resistance, and the selection of one gene by an antibiotic can facilitate the mutation of another gene, further enhancing the evolution of carbapenem resistance.
Article
Microbiology
Yanqin Huang, Karol Sokolowski, Amisha Rana, Nidhi Singh, Jiping Wang, Ke Chen, Yinzhi Lang, Jieqiang Zhou, Neera Kadiyala, Fiorella Krapp, Egon A. Ozer, Alan R. Hauser, Jian Li, Jurgen B. Bulitta, Zackery P. Bulman
Summary: Combining aminoglycosides with ceftazidime/avibactam shows promising synergy against KPC-producing Klebsiella pneumoniae infections, leading to reduced bacterial counts. Selecting aminoglycosides based on genes or susceptibilities can enhance the pharmacodynamic activity of the combination therapy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Chunlei Wang, Jiankang Zhao, Zhibo Liu, Aihua Sun, Lingxiao Sun, Binbin Li, Binghuai Lu, Yingmei Liu, Bin Cao
Summary: This study describes the in vivo evolution of carbapenem and ceftazidime-avibactam resistance in a pneumonia patient by analyzing four longitudinal Klebsiella pneumoniae clinical isolates. The results showed a dynamic change of resistance phenotype and the emergence of resistance to ceftazidime-avibactam and carbapenems.
FRONTIERS IN MICROBIOLOGY
(2021)