期刊
ENERGIES
卷 5, 期 8, 页码 3019-3032出版社
MDPI
DOI: 10.3390/en5083019
关键词
duckweed; Landoltia punctata; pectinase; ethanol fermentation; surface response design; Saccharomyces cerevisiae
资金
- National Sci-tech Pillar R&D Program of China [2011BAD22B03]
- China Agriculture Research System [CARS-11-A-04]
- Innovative Programs of The Chinese Academy of Sciences [KSCX2-EW-J-22, KSCX2-EW-G-1-1]
Landoltia punctata, a widely distributed duckweed strain with the ability to accumulate starch, was used as a novel feedstock for bioethanol production by Saccharomyces cerevisiae. To improve ethanol production, pectinase pretreatment was used to release much more glucose from L. punctata mash and the pretreatment conditions (enzyme loading, temperature and pretreatment time) for the duckweed were optimized by using a surface response design. The results showed that maximum glucose yield was 218.64 +/- 3.10 mg/g dry matter, which is a 142% increase compared to the untreated mash, with a pectinase dose of 26.54 pectin transeliminase unit/g mash at 45 degrees C for 300 min. Pectinase pretreatment apparently changed the ultrastructure of L. punctata, as evidenced by scanning electron microscopy analysis. Further fermentation experiments were performed and 30.8 +/- 0.8 g/L of ethanol concentration, 90.04% of fermentation efficiency and 2.20 g/L(/)h of productivity rate were achieved. This is the highest ethanol concentration reported to date using duckweed as the feedstock.
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