Article
Biochemistry & Molecular Biology
Yuluan Wang, Charlotte Soneson, Anna L. Malinowska, Artur Laski, Souvik Ghosh, Alexander Kanitz, Luca F. R. Gebert, Mark D. Robinson, Jonathan Hall
Summary: The study introduced a new class of miR-CLIP probes to capture targetomes of miR-124 and miR-132, revealing overlapping targetomes between isoforms and potential synergistic effects of isomirs. Interestingly, it was found that pre-miR-124 processed into isomiRs can repress targets more strongly than individual treatments with miR-124 and iso-miR-124. Mining the miR-CLIP targetome identified G-bulged target-sites regulated at the protein level by miR-124 but not isomiR-124, proposing a model involving AGO2 helix-7 for site engagement.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Oncology
Hossein Sendi, Mostafa Yazdimamaghani, Mengying Hu, Nikhila Sultanpuram, Jie Wang, Amber S. Moody, Ellie McCabe, Jiajie Zhang, Amanda Graboski, Liantao Li, Juan D. Rojas, Paul A. Dayton, Leaf Huang, Andrew Z. Wang
Summary: Efficient delivery of miR-122 to hepatocytes using a nanomedicine-based formulation has the potential to prevent and treat colorectal cancer liver metastasis. Mechanistically, it downregulates key genes involved in metastatic and cancer inflammation pathways, increases CD8(+)/CD4(+) T-cell ratio, and decreases immunosuppressive cell infiltration, making the liver more conducive to antitumor immune response.
Article
Endocrinology & Metabolism
Xiao-Yan Huang, Ji-Xiong Chen, Yi Ren, Li-Chun Fan, Wei Xiang, Xiao-Jie He
Summary: This study found that miR-122-enriched exosomes promote adipogenesis by regulating the VDR/SREBF1 axis. Inhibition of miR-122 can alleviate obesity.
Article
Orthopedics
K. M. Scott, D. J. Cohen, M. Hays, D. W. Nielson, M. W. Grinstaff, T. B. Lawson, B. D. Snyder, B. D. Boyan, Z. Schwartz
Summary: Both miR-122 and miR-451 were upregulated in OA in a rat bilateral anterior cruciate ligament transection (ACLT) model. miR-122 prevented the effects of IL-1 beta on rArCs, while miR-451 exacerbated them.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Biochemistry & Molecular Biology
Chuang Li, Fang Qin, Wei Wang, Yifan Ni, Mingyu Gao, Mingxiong Guo, Guihong Sun
Summary: The study revealed that miR-122-5p is selectively sorted and secreted into lung cancer EVs through binding to RNA-binding protein hnRNPA2B1, promoting the migration of liver cells. This may contribute to establishing a pre-metastatic micro-environment and hepatic metastasis of lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Harsha Ganesan, Suman K. K. Nandy, Antara Banerjee, Surajit Pathak, Hong Zhang, Xiao-Feng Sun
Summary: This study aimed to investigate the role of miR-122 in colorectal cancer (CRC) and identified a closely related group of genes influencing the prognosis of CRC. These genes can be silenced through RNA interference and may serve as effective therapeutic targets in understanding and treating CRC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Microbiology
Mamata Panigrahi, Michael A. Palmer, Joyce A. Wilson
Summary: This review explores the unique relationship between liver-specific microRNA miR-122 and Hepatitis C virus (HCV), as well as the role of miR-122 in the virus life cycle, viral tropism, and pathogenesis. The review also discusses the impact of anti-miR-122 therapy on viral replication and how viruses manipulate host factors during the initial stage of infection to establish a successful infection.
Article
Cell Biology
Yin Zhang, Ye-Ya Tan, Pei-Pei Chen, Hui Xu, Shu-Juan Xie, Shi-Jun Xu, Bin Li, Jun-Hao Li, Shun Liu, Jian-Hua Yang, Hui Zhou, Liang-Hu Qu
Summary: Liver development is a complex process involving the interplay of epigenetic regulators, transcription factors, and miRNAs. This study identified the genome-wide miRNA targetomes during mouse liver development and revealed a novel mechanism of terminal differentiation of hepatocytes regulated by the miR-122/Hippo pathway. The findings provide insights into the function of miR-122 in liver pathology, particularly in processes like inflammation, fibrosis, and hepatocellular carcinoma.
CELL DEATH & DISEASE
(2021)
Article
Biochemical Research Methods
Hideyuki Nakanishi, Hirohide Saito, Keiji Itaka
Summary: Synthetic mRNA is a valuable tool for medical applications, as it allows for transgene expression without the need for nuclear import and the risk of insertional mutagenesis. In this study, a protein-responsive translational regulation system was developed using Caliciviral VPg-based translational activator, inteins, and target protein-binding nanobodies. This system enables both activation and repression of translation in a target protein-dependent manner, with the ability to easily change the target protein. This versatile design offers a safe approach to target protein-responsive translational regulation therapeutics.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Cell Biology
Lori A. Passmore, Jeff Coller
Summary: Recent studies have shown that poly(A) tails play a complex role in the cytoplasm, including promoting translation and controlling mRNA stability through deadenylation. The length of poly(A) tails is correlated with translation rate and mRNA lifespan, indicating a significant role in gene regulation.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Heze Liu, Bo He, Wen Hu, Kexin Liu, Yongguo Dai, Dingmei Zhang, Hui Wang
Summary: Studies have demonstrated that prenatal dexamethasone exposure can lead to nonalcoholic fatty liver disease in adult male rat offspring, with mechanisms including inhibition of miR-122 expression, increased YY1 expression, and inhibition of the ACE2-MAS1 signaling pathway, resulting in increased hepatic triglyceride synthesis and decreased oxidative function.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Sabrina E. Iskandar, Lilly F. Chiou, Tina M. Leisner, Devan J. Shell, Jacqueline L. Norris-Drouin, Cyrus Vaziri, Kenneth H. Pearce, Albert A. Bowers
Summary: This study reports a method to identify covalent cyclic peptide inhibitors in mRNA display. By combining co- and post-translational library diversification strategies, cyclic libraries with reactive dehydroalanines (Dhas) were created and used for selections against two model targets. The most potent hits showed low nanomolar inhibitory activities and disrupted known protein-protein interactions with their selected targets. Overall, this study establishes Dhas as electrophiles for covalent inhibition and demonstrates the synergy of separate library diversification methods in mRNA display for novel applications like covalent inhibitor discovery.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Yuta Yoshino, Bhaskar Roy, Yogesh Dwivedi
Summary: Chronic stress is a key factor in major depressive disorder, and this study found that miR-218-5p plays a role in the mechanism of depression by inducing chromatin-based transcriptional changes of several CNS genes. The upregulation of miR-218-5p in response to chronic stress was observed in the prefrontal cortex of rats. This research provides insights into the potential therapeutic target of miR-218-5p in clinical depression.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Jianling Xie, Eric P. Kusnadi, Luc Furic, Luke A. Selth
Summary: Estrogen and androgen receptors are key drivers in breast and prostate cancer, regulating mRNA translation to influence cellular protein production and enhance cancer cell growth. Targeting mRNA translation could be a potential strategy for treating breast and prostate cancer.
Article
Cell Biology
Wei Yan, Minghui Cao, Xianhui Ruan, Li Jiang, Sylvia Lee, Adriana Lemanek, Majid Ghassemian, Donald P. Pizzo, Yuhao Wan, Yueqing Qiao, Andrew R. Chin, Erika Duggan, Dong Wang, John P. Nolan, Jeffrey D. Esko, Simon Schenk, Shizhen Emily Wang
Summary: Breast cancer suppresses O-GlcNAc protein modification in muscle through miR-122, leading to dysregulated skeletal muscle proteolysis with aberrant Ca2+ levels and calpain protease activation.
NATURE CELL BIOLOGY
(2022)