期刊
ENDOCRINOLOGY
卷 153, 期 5, 页码 2082-2087出版社
ENDOCRINE SOC
DOI: 10.1210/en.2011-2019
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资金
- National Institutes of Health [DK50610]
- Diabetes Research Center at the University of Chicago [DK020595]
- Canadian Institutes of Health Research [MOP-14682]
Dysfunctional islet amyloid polypeptide (IAPP) biosynthesis and/or processing are thought contribute to formation of islet amyloid in type 2 diabetes. However, it is unclear how normal pro-IAPP biosynthesis and processing are regulated to be able to define such dysfunction. Here, it was found that acute exposure to high glucose concentrations coordinately regulated the biosynthesis of pro-IAPP, proinsulin, and its proprotein convertase PC1/3 in normal isolated rat islets, without affecting their respective mRNA levels. Pro-7B2 biosynthesis, like that of pro-PC2, did not appreciably change, but this was likely due to a much higher expression in pancreatic alpha-cells masking glucose regulation of their biosynthesis in beta-cells. Biosynthesis of pro-SAAS, the putative PC1/3 chaperone, was unaffected by glucose, consistent with its scarce expression in beta-cells. We conclude that translational control of pro-IAPP biosynthesis, in parallel to the pro-PC1/3, pro-PC2, and pro-7B2 proprotein-processing endopeptidases/chaperones, is the predominate mechanism to produce IAPP in islet beta-cells. (Endocrinology 153: 2082-2087, 2012)
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