期刊
EMBO REPORTS
卷 14, 期 1, 页码 73-79出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/embor.2012.182
关键词
chromatin modifications; embryonic stem cells; endogenous retroviruses; epigenetic silencing; primer-binding site
资金
- NCI [R37 CA 30488]
- NYSTEM grant from the New York State Department of Health [N08G-152, C024329]
Embryonic stem cells repress retroviral infection through transcriptional silencing of proviral DNAs. We characterized two distinct mechanisms of silencing in embryonic mouse cells infected by Moloney murine leukaemia virus (MLV): a highly efficient one targeting the proline transfer RNA primer-binding site (PBSpro), and a less efficient one operating independently of the PBS. Rare virus-expressing populations were isolated, and the timing and efficiency of establishment of silencing were determined. Superinfection of the selected virus-expressing cells with a second virus carrying a distinguishable reporter revealed that the PBSpro-directed silencing was still largely intact, whereas the PBS-independent silencing was partially reduced. The timing and stability of silencing, and the associated chromatin modifications on newly established and endogenous proviruses were determined. The results indicate that epigenetic mechanisms with different specificity and efficiency are used to silence the exogenous retroviral sequences in embryonic cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据