期刊
EMBO REPORTS
卷 11, 期 12, 页码 936-942出版社
WILEY
DOI: 10.1038/embor.2010.164
关键词
exosome; RNA degradation; cryo-electron microscope; macromolecular complex
资金
- European Union
- EUxosome European Science Foundation
- EMBO [N N301 160335, N N301 251336]
- Foundation for Polish Science
- Wellcome Trust
- Max Planck Gesellschaft
- German Research Foundation
- Federation of European Biochemical Societies and Human Frontier Science Program
- Medical Research Council
- [SFB646]
- BBSRC [BB/F010281/1] Funding Source: UKRI
- MRC [G0600084] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F010281/1] Funding Source: researchfish
- Medical Research Council [G0600084] Funding Source: researchfish
The eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models of the ten-subunit Saccharomyces cerevisiae exosome in the unbound and RNA-bound states. In the RNA-bound structures, extra density that is visible at the entry and exit sites of the exosome channel indicates that a substrate-threading mechanism is used by the eukaryotic exosome. This channelling mechanism seems to be conserved in exosome-like complexes from all domains of life, and might have been present in the most recent common ancestor.
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