期刊
EMBO REPORTS
卷 10, 期 8, 页码 851-856出版社
WILEY
DOI: 10.1038/embor.2009.96
关键词
SNARE; VAMP3; VAMP4; VAMP7; VAMP8
资金
- Medical Research Council
- Medical Research Council [G120/952] Funding Source: researchfish
- MRC [G120/952] Funding Source: UKRI
The sorting of post-Golgi R-SNAREs (vesicle-associated membrane protein (VAMP) 1, 2, 3, 4, 7 and 8) is still poorly understood. To address this, we developed a system to investigate their localization, trafficking and cell-surface levels. Here, we show that the distribution and internalization of VAMPs 3 and 8 are determined solely through a new conserved mechanism that uses coiled-coil interactions, and that VAMP4 does not require these interactions for its trafficking. We propose that VAMPs 3 and 8 are trafficked while in a complex with Q-SNAREs. We also show that the dileucine motif of VAMP4 is required for both its internalization and retrieval to the trans-Golgi network. However, when the dileucine motif is mutated, the construct can still be internalized potentially through coiled-coil interactions with Q-SNAREs.
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