Article
Chemistry, Applied
Ahmad Nasrollahzadeh, Samaneh Mollaei Tavani, Edris Arjeh, Seid Mahdi Jafari
Summary: Conjugated linoleic acid (CLA) has gained significant attention as a health-promoting compound. Microbial biosynthesis of CLA is a practical and controllable method for commercial production, and understanding the interplay of different factors is essential in optimizing its quantity and composition.
Article
Biochemistry & Molecular Biology
Xiao Li, Tengfei Zheng, Yu Zhang, Yachao Zhao, Fengming Liu, Shen Dai, Xiaolin Liu, Mei Zhang
Summary: This study found that DKK1 promotes smooth muscle cell foam cell formation during atherosclerosis by reducing CYP4A11-20-HETE/SREBP2-mediated ABCA1 expression.
Article
Agriculture, Dairy & Animal Science
L. Vogel, M. Gnott, C. Kroeger-Koch, S. Goers, J. M. Weitzel, E. Kanitz, A. Hoeflich, A. Tuchscherer, A. Troescher, J. J. Gross, R. M. Bruckmaier, A. Starke, L. Bachmann, H. M. Hammon
Summary: The study found that treating cows with CLA can increase the concentration of plasma glucose and insulin, while stimulating the action of the somatotropic axis; whereas treating with EFA can stimulate the increase in eGP, but does not affect the mRNA abundance related to eGP.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Food Science & Technology
Yongchao Mei, Haiqin Chen, Bo Yang, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: This article reviews the current knowledge about Bifidobacterium CLA producers, the complex factors regulating CLA production in Bifidobacterium, the role of CLA production in Bifidobacterium, and the potential mechanism underlying Bifidobacterium CLA production. It offers novel insights into the production of food-grade CLA as well as the rational design of health-promoting fermented foods or synbiotics.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Lei Li, Yun Fang, Yongmei Xia, Chunling Bo, Ye Fan
Summary: By using specific small molecules, the pH window of fatty acid vesicles (FAV) can be extended, allowing for the potential application of FAV in drug delivery systems.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Review
Pharmacology & Pharmacy
Elisabetta Murru, Gianfranca Carta, Claudia Manca, Valeria Sogos, Marco Pistis, Miriam Melis, Sebastiano Banni
Summary: CLA, as a group of isomers of linoleic acid, plays a crucial role in brain health by binding to PPARα, regulating fatty acid metabolism and exerting anti-neuroinflammatory effects. Further studies are needed to explore the potential anti-inflammatory activity of dietary CLA, especially in neurodegenerative diseases and neuropsychiatric disorders with a neuroinflammatory basis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Applied
Ana Luiza Fontes, Bruna Neves, Tiago Conde, Daniela Couto, Ligia Leao Pimentel, Luis Miguel Rodriguez-Alcala, M. Rosario Domingues, Ana Maria Gomes
Summary: This study investigated potential fatty acid byproducts during microbial enrichment of CLA/CLNA and found that no additional side-fatty acid metabolites were released except for stearic acid in the presence of linoleic acid.
Article
Agriculture, Multidisciplinary
Baixi Zhang, Lijuan Ni, Xiaoshu Tang, Xuemei Chen, Bo Hu
Summary: This study achieved a high yield of t10, c12-CLA through genetic modification and reduced its degradation rate. The highest t10, c12-CLA yield reported to date was obtained in a bioreactor using low-cost carbon sources.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Yafang He, Kun Xu, Yunfeng Li, Huan Chang, Xia Liao, Hang Yu, Tian Tian, Chao Li, Yuan Shen, Qian Wu, Xin Liu, Lin Shi
Summary: The study found that CLA can improve body composition, with changes in the metabolome predicting responsiveness to intervention. Post-intervention metabolite alterations were associated with changes in body composition, showing the potential of baseline metabolite profiling in predicting CLA-induced benefits.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Microbiology
Nho-Eul Song, Na-Jeong Kim, Young-Hun Kim, Sang-Ho Baik
Summary: The study evaluated the probiotic properties of 10 strains of high CLA-producing lactic acid bacteria isolated from fermented seafood. Some of these strains produced significant amounts of CLA and demonstrated potential as probiotics with immune-modulating and antimicrobial activities in both cell and animal models.
Article
Medicine, Research & Experimental
Aiyang Liu, Ruizhi Li, Farah Zaaboul, Mengxue He, Xue Li, Jiachen Shi, Yuanfa Liu, Yong -Jiang Xu
Summary: This study aimed to investigate the effect of astaxanthin on foam cells and identified the protein expression changes using proteomic approaches. The results showed that total cholesterol and free cholesterol increased in foam cells treated with astaxanthin. Proteomics analysis revealed the involvement of PI3K/CDC42 and PI3K/RAC1/TGF-beta 1 pathways in lipid metabolism and cholesterol efflux, leading to reduced inflammation in foam cells. These findings provide new insights into the mechanism of astaxanthin in regulating lipid metabolism in macrophage foam cells.
Article
Food Science & Technology
Neha Baliyan, Antim Kumar Maurya, Anil Kumar, Vijai Kant Agnihotri, Rakshak Kumar
Summary: This study isolated probiotic strains from raw cow milk of the Lahaul valley capable of producing cis-9, trans-11 CLA isomer, and exhibiting antioxidant and food formulation abilities. Genome sequencing revealed the presence of antibiotic-resistance and virulence genes in Enterococcus faecalis LJM:05. The strain Latilactobacillus curvatus LGM:16 was used to develop curdled milk with superior probiotic features, antioxidant activity, and CLA-producing ability.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiao Li, Tengfei Zheng, Yu Zhang, Yachao Zhao, Fengming Liu, Shen Dai, Xiaolin Liu, Mei Zhang
Summary: This study reveals that DKK1 promotes foam cell formation in smooth muscle cells (SMCs) during atherosclerosis by reducing CYP4A11-20-HETE/SREBP2-mediated ABCA1 expression. This finding is important for understanding the pathogenesis of atherosclerosis and developing related therapeutic strategies.
Article
Chemistry, Applied
Junjun Qing, Cheng Peng, Hongbing Chen, Haixing Li, Xiaohua Liu
Summary: The study aimed to investigate the impact of fermentation conditions on c9,t11-conjugated linoleic acid (CLA) synthesis by Lactobacillus casei, as well as its effects on whey syneresis, water holding capacity (WHC), and texture characteristics of set yogurt. The addition of 0.1% linoleic acid (LA) decreased whey syneresis by about 30%. UV-Vis absorption spectroscopy, 3D fluorescence spectroscopy, and CD spectroscopy revealed the interaction between LA and casein (CS), fi-lactoglobulin (fi-Lg), and bovine serum albumin (BSA). LA changed the microenvironment and polarity around amino acids, as well as the conformation of the three milk proteins. Scanning electron microscope (SEM) analysis showed that the addition of LA resulted in a more uniform and compact microstructure of the set yogurt, suggesting that LA can promote the crosslink of milk proteins, which may explain the reduction in whey syneresis.
Article
Agriculture, Dairy & Animal Science
Daria Mrugala, Jessica L. Leatherwood, Elizabeth F. Morris, Emily C. Dickson, Christine M. Latham, Randi N. Owen, Marcy M. Beverly, Stanley F. Kelley, Sarah H. White-Springer
Summary: Conjugated linoleic acid (CLA) supplementation in lightly exercising horses may result in mitochondrial adaptations and protection against myofiber perturbation in skeletal muscle. Significant differences were observed in mitochondrial enzyme activities and antioxidant status between horses supplemented with soybean oil and CLA. Superoxide dismutase activity tended to increase over time in the soybean oil group, while glutathione peroxidase activity tended to be higher in the soybean oil group compared to the CLA group at week 12.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Neurosciences
Jayanth Anantha, Susan R. Goulding, Eszter Tuboly, Adam G. O'Mahony, Gerard M. Moloney, Gareth Lomansey, Cathal M. McCarthy, Louise M. Collins, Aideen M. Sullivan, Gerard W. O'Keeffe
Summary: Parkinson's disease is a neurodegenerative disease characterized by the progressive degeneration of midbrain dopaminergic neurons and axonal degeneration. Research suggests that the neurotrophic factor NME1 may have potential as a therapeutic agent for axonal protection in PD, enhancing mitochondrial function and promoting neurite growth in cell models.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Neurosciences
Erin McCarthy, Aaron Barron, Noelia Morales-Prieto, Martina Mazzocchi, Cathal M. McCarthy, Louise M. Collins, Aideen M. Sullivan, Gerard W. O'Keeffe
Summary: The study suggests a potential therapeutic target for Parkinson's disease in ZNHIT1, which can protect against dopaminergic degeneration and mitochondrial dysfunction induced by alpha-synuclein. Overexpression of ZNHIT1 increases cellular ATP synthesis and prevents impairments in mitochondrial function caused by alpha-synuclein.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Larissa Traxler, Joseph R. Herdy, Davide Stefanoni, Sophie Eichhorner, Silvia Pelucchi, Attila Szucs, Alice Santagostino, Yongsung Kim, Ravi K. Agarwal, Johannes C. M. Schlachetzki, Christopher K. Glass, Jessica Lagerwall, Douglas Galasko, Fred H. Gage, Angelo D'Alessandro, Jerome Mertens
Summary: This study investigated the metabolic changes and transcriptional alterations in sporadic Alzheimer's disease (AD) utilizing induced neurons (iNs) from AD patients. The pathological isoform switching of PKM2 was found to contribute to neuronal vulnerability and loss in AD. Chemical modulation of PKM2 could reverse these changes and enhance neuronal resilience against cell death.
Article
Immunology
Zsolt Czimmerer, Laszlo Halasz, Bence Daniel, Zsofia Varga, Krisztian Bene, Apolka Domokos, Marten Hoeksema, Zeyang Shen, Wilhelm K. Berger, Timea Cseh, Karoly Jambrovics, Zsuzsanna Kolostyak, Ferenc Fenyvesi, Judit Varadi, Szilard Poliska, Gyorgy Hajas, Istvan Szatmari, Christopher K. Glass, Attila Bacsi, Laszlo Nagy
Summary: Prior exposure to microenvironmental signals can alter the response of macrophages. IL-4-polarized macrophages were found to exhibit hyperinflammatory gene expression upon LPS exposure, contrary to previous beliefs. This extended synergy was supported by epigenomic remodeling, NF-kappa B-p65 cistrome expansion, and increased enhancer activity.
Article
Neurosciences
Kristina Battis, Jazmin B. Florio, Michael Mante, Addison Lana, Isabel Naumann, Carina Gauer, Vera Lambrecht, Simon Julian Mueller, Isidoro Cobo, Bethany Fixsen, Ha Yeon Kim, Eliezer Masliah, Christopher K. Glass, Johannes C. M. Schlachetzki, Robert A. Rissman, Juergen Winkler, Alana Hoffmann
Summary: This study reveals the complex role of myeloid cells in multiple system atrophy and emphasizes the importance of balancing their beneficial and adverse effects before clinical translation.
JOURNAL OF NEUROSCIENCE
(2022)
Review
Gastroenterology & Hepatology
Daniel Q. Huang, Michael Downes, Ronald M. Evans, Joseph L. Witztum, Christopher K. Glass, Rohit Loomba
Summary: The global burden of nonalcoholic fatty liver disease (NAFLD) is increasing, with individuals with NAFLD at higher risk for cardiovascular disease, sharing multiple disease mechanisms.
SEMINARS IN LIVER DISEASE
(2022)
Article
Cell & Tissue Engineering
Joseph R. Herdy, Larissa Traxler, Ravi K. Agarwal, Lukas Karbacher, Johannes C. M. Schlachetzki, Lena Boehnke, Dina Zangwill, Doug Galasko, Christopher K. Glass, Jerome Mertens, Fred H. Gage
Summary: The concept of senescence extends beyond proliferating cells, as senescence-like features have been observed in neurons, including those affected by Alzheimer's disease. Targeting senescent neurons could be a strategy for preventing or treating AD.
Article
Neurosciences
Johannes C. M. Schlachetzki, Yi Zhou, Christopher K. Glass
Summary: Cognitive impairment is prevalent in individuals with HIV despite antiretroviral therapy, with evidence suggesting that the brain may serve as a sanctuary for HIV persistence. Recent advances in understanding the diversity of microglia in HIV, including their epigenome, transcriptome, and function, highlight their potential role in driving HIV-associated neurocognitive disorders.
CURRENT OPINION IN NEUROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kei-ichiro Arimoto, Sayuri Miyauchi, Ty D. Troutman, Yue Zhang, Mengdan Liu, Samuel A. Stoner, Amanda G. Davis, Jun-Bao Fan, Yi-Jou Huang, Ming Yan, Christopher K. Glass, Dong-Er Zhang
Summary: Immunotherapy is an effective cancer treatment, but not for all patients, prompting the need for alternative strategies. Inducing cancer immunogenic cell death (ICD) shows promise in promoting robust immune responses against tumor-associated antigens. Depletion of USP18, a negative regulator of interferon signaling, selectively induces ICD in cancer cells, suggesting targeting USP18 as a potential cancer immunotherapy.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Reem Abdel-Haq, Johannes C. M. Schlachetzki, Joseph C. Boktor, Thaisa M. Cantu-Jungles, Taren Thron, Mengying Zhang, John W. Bostick, Tahmineh Khazaei, Sujatha Chilakala, Livia H. Morais, Greg Humphrey, Ali Keshavarzian, Jonathan E. Katz, Matthew Thomson, Rob Knight, Viviana Gradinaru, Bruce R. Hamaker, Christopher K. Glass, Sarkis K. Mazmanian
Summary: Parkinson's disease is a movement disorder characterized by neuroinflammation, alpha-synuclein pathology, and neurodegeneration. The gut microbiome, which is altered in PD, may impact motor and GI symptoms. Feeding a prebiotic high-fiber diet can improve motor deficits and reduce alpha-synuclein aggregation in PD-like mice, potentially through its effects on the gut microbiome and microglial activation.
Article
Physiology
Aaron Barron, Samprikta Manna, Colm J. McElwain, Andrea Musumeci, Fergus P. McCarthy, Gerard W. O'Keeffe, Cathal M. McCarthy
Summary: Pre-eclampsia (PE) is a common and serious hypertensive disorder of pregnancy that can have detrimental effects on both maternal and neonatal health. Exposure to maternal serum from women with PE has been found to increase neurite growth and mitochondrial function in neuronal cells, which could potentially contribute to the development of neurodevelopmental disorders in offspring.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemical Research Methods
Samprikta Manna, Julia Scheel, Aisling Noone, Colm J. J. McElwain, Caitriona Scaife, Shailendra Gupta, Jane English, Cathal McCarthy, Fergus P. P. McCarthy
Summary: This study characterized the protein networks of healthy term placentas using mass spectrometry. The results showed significant differences in protein expression and composition among different sub-anatomical regions of the placenta. Decreased anti-senescent proteins in the maternal region and increased proteins related to energy metabolism in the middle and fetal regions were observed. Serial sectioning methodology is recommended for consistent placental research.
CLINICAL PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Samprikta Manna, Colm J. J. Mc Elwain, Gillian M. M. Maher, Marta Giralt Martin, Andrea Musumeci, Fergus P. P. McCarthy, Cathal McCarthy
Summary: This study investigated the cellular senescence phenotypes of pre-eclampsia and intrauterine growth restriction (IUGR) pregnancies by measuring multiple biomarkers of senescence. The results showed signs of premature senescence in IUGR pregnancies, while pre-eclampsia activated cell cycle checkpoint regulators but with a phenotype of cell repair and proliferation rather than senescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Neurosciences
Rosa C. Paolicelli, Amanda Sierra, Beth Stevens, Marie-Eve Tremblay, Adriano Aguzzi, Bahareh Ajami, Ido Amit, Etienne Audinat, Ingo Bechmann, Mariko Bennett, Frederick Bennett, Alain Bessis, Knut Biber, Staci Bilbo, Mathew Blurton-Jones, Erik Boddeke, Dora Brites, Bert Brone, Guy C. Brown, Oleg Butovsky, Monica J. Carson, Bernardo Castellano, Marco Colonna, Sally A. Cowley, Colm Cunningham, Dimitrios Davalos, Philip L. De Jager, Bart de Strooper, Adam Denes, Bart J. L. Eggen, Ukpong Eyo, Elena Galea, Sonia Garel, Florent Ginhoux, Christopher K. Glass, Ozgun Gokce, Diego Gomez-Nicola, Berta Gonzalez, Siamon Gordon, Manuel B. Graeber, Andrew D. Greenhalgh, Pierre Gressens, Melanie Greter, David H. Gutmann, Christian Haass, Michael T. Heneka, Frank L. Heppner, Soyon Hong, David A. Hume, Steffen Jung, Helmut Kettenmann, Jonathan Kipnis, Ryuta Koyama, Greg Lemke, Marina Lynch, Ania Majewska, Marzia Malcangio, Tarja Malm, Renzo Mancuso, Takahiro Masuda, Michela Matteoli, Barry W. McColl, Veronique E. Miron, Anna Victoria Molofsky, Michelle Monje, Eva Mracsko, Agnes Nadjar, Jonas J. Neher, Urte Neniskyte, Harald Neumann, Mami Noda, Bo Peng, Francesca Peri, V. Hugh Perry, Phillip G. Popovich, Clare Pridans, Josef Priller, Marco Prinz, Davide Ragozzino, Richard M. Ransohoff, Michael W. Salter, Anne Schaefer, Dorothy P. Schafer, Michal Schwartz, Mikael Simons, Cody J. Smith, Wolfgang J. Streit, Tuan Leng Tay, Li-Huei Tsai, Alexei Verkhratsky, Rommy von Bernhardi, Hiroaki Wake, Valerie Wittamer, Susanne A. Wolf, Long-Jun Wu, Tony Wyss-Coray
Summary: Microglial research has made significant progress, but the current classification system fails to accurately describe their diversity, leading to misconceptions about their functions. To address this issue, a group of multidisciplinary experts has proposed a naming framework and recommendations to help researchers better understand and describe the different states and functions of microglia.
Meeting Abstract
Clinical Neurology
Emily Hansen, Anna S. Warden, Cristina Mora, Gabriela Ramirez, Samantha Mak, Sanjana Narayan, Samantha Trescott, Shreya Shriram, Zahara Keulen, Christopher K. Glass, Nicole G. Coufal
ANNALS OF NEUROLOGY
(2022)