期刊
EMBO JOURNAL
卷 32, 期 21, 页码 2872-2883出版社
WILEY
DOI: 10.1038/emboj.2013.206
关键词
fimbriae; gene regulation; Salmonella
资金
- Public Health Service Grant [AI040124, GM059969, AI097116, AI074693]
- Deutsche Forschungsgemeinschaft
A hierarchical control of fimbrial gene expression limits laboratory grown cultures of Salmonella enterica serovar typhimurium (S. typhimurium) to the production of type I fimbriae encoded by the fimAICDHF operon. Here we show that an unlikely culprit, namely the 50-untranslated region (50-UTR) of a messenger (m) RNA, coordinated the regulation. Binding of CsrA to the 50-UTR of the pefACDEF transcript was required for expression of plasmid-encoded fimbriae. The 50-UTR of the fimAICDHF transcript cooperated with two small untranslated RNAs, termed CsrB and CsrC, in antagonizing the activity of the RNA binding protein CsrA. Through this post-transcriptional mechanism, the 50-UTR of the fimAICDHF transcript prevented production of PefA, the major structural subunit of plasmid-encoded fimbriae. This regulatory mechanism limits the costly expression of plasmid-encoded fimbriae to host environments in a mouse model. Collectively, our data suggest that the 50-UTR of an mRNA coordinates a hierarchical control of fimbrial gene expression in S. typhimurium.
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