Article
Endocrinology & Metabolism
Mark N. Adams, Laura V. Croft, Aaron Urquhart, Mohamed Ashick Mohamed Saleem, Anja Rockstroh, Pascal H. G. Duijf, Patrick B. Thomas, Genevieve P. Ferguson, Idris Mohd Najib, Esha T. T. Shah, Emma Bolderson, Shivashankar Nagaraj, Elizabeth D. Williams, Colleen C. Nelson, Kenneth J. O'Byrne, Derek J. Richard
Summary: This study evaluated the role of hSSB1/NABP2 in modulating the cellular response to androgens and ionizing radiation in prostate cancer. The expression of hSSB1 in prostate cancer is correlated with genomic instability and it regulates pathways involved in cell cycle progression and transcription. Additionally, hSSB1 is involved in modulating androgen response through transcriptional regulation. Exploiting hSSB1 in prostate cancer treatment might improve patient outcomes in ADT and/or radiotherapy.
Review
Genetics & Heredity
Yongxin Li, Yan Tong, Jiaqi Liu, Jianlin Lou
Summary: DNA is crucial for organism development and function. MiRNA, a type of non-coding RNA, may play an important role in the DNA damage response by influencing processes like cell cycle, DNA repair, and apoptosis, thereby impacting genomic stability and tumorigenesis.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Yuejing Jiang, Xiaoji Cong, Shangwen Jiang, Ying Dong, Lei Zhao, Yi Zang, Minjia Tan, Jia Li
Summary: AMP-activated protein kinase (AMPK) plays regulatory roles in DNA repair through phosphorylation of various substrates and modulation of histone acetylation. Specifically, AMPK promotes apoptosis through phosphorylation of apoptosis-stimulating of p53 protein 2 (ASPP2), and regulates histone acetylation through phosphorylation of histone deacetylase 9 (HDAC9). Additionally, disrupting histone acetylation enhances the sensitivity of AMPK-deficient cells to irradiation.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Cell Biology
Qilin Long, Marek Sebesta, Katerina Sedova, Vojtech Haluza, Adele Alagia, Zhichao Liu, Richard Stefl, Monika Gullerova
Summary: In this study, it was discovered that the damage-activated tyrosine kinase c-Abl phosphorylates hSSB1, enabling its interaction with Y1P RNAPII at DSBs, and participates in the regulation of transcription at DSBs. The SOSS1 complex, together with RNAPII, forms dynamic liquid-like repair compartments at DSBs in response to DNA damage. This study highlights the importance of the SOSS1 complex in R-loop-dependent DNA damage response.
Article
Oncology
Jiajing Niu, Jiamei Wang, Qi Zhang, Zhihua Zou, Yushuang Ding
Summary: The study revealed that sublethal doses of cinobufagin suppressed the viability of cancer cells by inducing oxidative stress, leading to DNA damage and cell cycle regulation that ultimately resulted in the induction of apoptosis.
CANCER CELL INTERNATIONAL
(2021)
Article
Oncology
Ming Tang, Joshua T. Burgess, Mark Fisher, Didier Boucher, Emma Bolderson, Neha S. Gandhi, Kenneth J. O'Byrne, Derek J. Richard, Amila Suraweera
Summary: This study aimed to explore the binding pose of COMMD4-H2B and develop a H2B peptide that disrupts the COMMD4-H2B interaction, which could serve as a potential therapeutic target for non-small cell lung cancer (NSCLC).
BRITISH JOURNAL OF CANCER
(2023)
Article
Engineering, Environmental
Hongrui Guo, Yujuan Ouyang, Jiaqi Wang, Hengmin Cui, Huidan Deng, Xinyue Zhong, Zhijie Jian, Huan Liu, Jing Fang, Zhicai Zuo, Xun Wang, Ling Zhao, Yi Geng, Ping Ouyang, Huaqiao Tang
Summary: Copper is essential but over-exposure can lead to adverse health effects. CuSO4 was found to induce spermatogenesis disorder through oxidative stress-mediated DNA damage and apoptosis, impairing male reproductive function.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Yanting Yang, Xiuhong Zhu, Guohua Yu, Jinbo Ma
Summary: This study demonstrates that Pyxinol can mitigate cisplatin-induced renal injury by attenuating DNA damage response and tubular cell apoptosis. Furthermore, Pyxinol can enhance the in vivo anti-tumor efficacy of cisplatin against xenograft tumors in nude mice. The combination of Pyxinol with cisplatin may represent a beneficial adjunct therapy for cisplatin-based chemotherapeutic regimens in the clinic.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Cell Biology
Karla E. Lopez, Lisa Bouchier-Hayes
Summary: Members of the caspase family have established roles in cell death, but recent evidence suggests they also play a separate role in the DNA damage response (DDR). This review explores the apoptotic and non-apoptotic functions of caspases in the DDR and their impact on genomic stability and cancer treatment.
Article
Pharmacology & Pharmacy
Sara Cimini, Giorgio Giaccone, Fabrizio Tagliavini, Matteo Costantino, Paola Perego, Giacomina Rossi
Summary: Tau protein, which plays a key role in neurons, is associated with neurodegenerative diseases and has been found to be linked to cancer. Mutations in the MAPT gene, which codes for tau, lead to dysregulation of cancer-relevant processes and decreased sensitivity to microtubule and DNA perturbation.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Cell Biology
Yu Jia, Zixiu Cheng, Sakshibeedu R. Bharath, Qiangzu Sun, Nannan Su, Jun Huang, Haiwei Song
Summary: The research reveals the crucial role of the interaction between INTS3 and INTS6 in DSB repair, where INTS3 dimerization plays a key role in recognizing long ssDNA, and disruption of INTS3c/INTS6c interaction can impair the DSB repair process.
Article
Biochemistry & Molecular Biology
Su-Ling Liu, Kun-Han Yang, Che-Wei Yang, Min-Yu Lee, Ya-Ting Chuang, Yan-Ning Chen, Fang-Rong Chang, Chung-Yi Chen, Hsueh-Wei Chang
Summary: Burmannic acid (BURA) derived from Indonesian cinnamon selectively inhibits oral cancer cell proliferation with little toxicity to normal cells, through perturbing cell cycle, inducing DNA damage, and promoting apoptosis. Oxidative stress is identified as a critical factor in these anticancer effects.
Article
Multidisciplinary Sciences
Alejandro Damian La Greca, Nelba Perez, Sheila Castaneda, Paula Melania Milone, Maria Agustina Scarafia, Alan Miqueas Mobbs, Ariel Waisman, Lucia Natalia Moro, Gustavo Emilio Sevlever, Carlos Daniel Luzzani, Santiago Gabriel Miriuka
Summary: Cell death experiments are crucial for drug development, and predicting cell death with neural networks can outperform human ability. A simple Python tool has been developed by the research team for broadly detecting cell death.
Article
Biology
David Murray, Razmik Mirzayans
Summary: The cellular response to genotoxic and/or oxidative stress caused by agents such as ionizing radiation exhibits a nonlinear relationship, with p53 playing a key role in coordinating these responses. In solid tumor-derived cell lines, moderate doses of radiation primarily invoke a dose-dependent cytostatic response.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dimitra T. Stefanou, Marousa Kouvela, Dimitris Stellas, Konstantinos Voutetakis, Olga Papadodima, Konstantinos Syrigos, Vassilis L. Souliotis
Summary: The deregulated DNA damage response network is associated with the onset and progression of lung cancer. This study found that lung cancer patients have higher levels of endogenous DNA damage, which may be caused by oxidative stress and defective DNA repair mechanisms. The findings suggest that oxidative stress and DDR-related aberrations contribute to the accumulation of endogenous DNA damage in lung cancer patients.
Article
Multidisciplinary Sciences
Koen K. A. Van Rompay, Lark L. Coffey, Tania Kapoor, Anna Gazumyan, Rebekah I. Keesler, Andrea Jurado, Avery Peace, Marianna Agudelo, Jennifer Watanabe, Jodie Usachenko, Anil Singapuri, Ramya Immareddy, Amir Ardeshir, Jackson B. Stuart, Stylianos Bournazos, Jeffrey V. Ravetch, Paul J. Balderes, Ivo C. Lorenz, Shannon R. Esswein, Jennifer R. Keeffe, Pamela J. Bjorkman, Qiao Wang, Charles M. Rice, Margaret R. MacDonald, Michel C. Nussenzweig, Davide F. Robbiani
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Shannon R. Esswein, Harry B. Gristick, Andrea Jurado, Avery Peace, Jennifer R. Keeffe, Yu E. Lee, Alisa V. Voll, Mohsan Saeed, Michel C. Nussenzweig, Charles M. Rice, Davide F. Robbiani, Margaret R. MacDonald, Pamela J. Bjorkman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Christopher O. Barnes, Anthony P. West, Kathryn E. Huey-Tubman, Magnus A. G. Hoffmann, Naima G. Sharaf, Pauline R. Hoffman, Nicholas Koranda, Harry B. Gristick, Christian Gaebler, Frauke Muecksch, Julio C. Cetrulo Lorenzi, Shlomo Finkin, Thomas Hagglof, Arlene Hurley, Katrina G. Millard, Yiska Weisblum, Fabian Schmidt, Theodora Hatziioannou, Paul D. Bieniasz, Marina Caskey, Davide F. Robbiani, Michel C. Nussenzweig, Pamela J. Bjorkman
Article
Microbiology
Qiao Wang, Eleftherios Michailidis, Yingpu Yu, Zijun Wang, Arlene M. Hurley, Deena A. Oren, Christian T. Mayer, Anna Gazumyan, Zhenmi Liu, Yunjiao Zhou, Till Schoofs, Kai-hui Yao, Jan P. Nieke, Jianbo Wu, Qingling Jiang, Chenhui Zou, Mohanmmad Kabbani, Corrine Quirk, Thiago Oliveira, Kalsang Chhosphel, Qianqian Zhang, William M. Schneider, Cyprien Jahan, Tianlei Ying, Jill Horowitz, Marina Caskey, Mila Jankovic, Davide F. Robbiani, Yumei Wen, Ype P. de Jong, Charles M. Rice, Michel C. Nussenzweig
CELL HOST & MICROBE
(2020)
Editorial Material
Immunology
Davide F. Robbiani
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Article
Immunology
Alexandra Schafer, Frauke Muecksch, Julio C. C. Lorenzi, Sarah R. Leist, Melissa Cipolla, Stylianos Bournazos, Fabian Schmidt, Rachel M. Maison, Anna Gazumyan, David R. Martinez, Ralph S. Baric, Davide F. Robbiani, Theodora Hatziioannou, Jeffrey Ravetch, Paul D. Bieniasz, Richard A. Bowen, Michel C. Nussenzweig, Timothy P. Sheahan
Summary: The study shows that combined use of hu-mAbs is effective for prevention and therapy of SARS-CoV-2 infection, but in vivo protection is influenced by intact effector function.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Christian Gaebler, Zijun Wang, Julio C. C. Lorenzi, Frauke Muecksch, Shlomo Finkin, Minami Tokuyama, Alice Cho, Mila Jankovic, Dennis Schaefer-Babajew, Thiago Y. Oliveira, Melissa Cipolla, Charlotte Viant, Christopher O. Barnes, Yaron Bram, Gaelle Breton, Thomas Hagglof, Pilar Mendoza, Arlene Hurley, Martina Turroja, Kristie Gordon, Katrina G. Millard, Victor Ramos, Fabian Schmidt, Yiska Weisblum, Divya Jha, Michael Tankelevich, Gustavo Martinez-Delgado, Jim Yee, Roshni Patel, Juan Dizon, Cecille Unson-O'Brien, Irina Shimeliovich, Davide F. Robbiani, Zhen Zhao, Anna Gazumyan, Robert E. Schwartz, Theodora Hatziioannou, Pamela J. Bjorkman, Saurabh Mehandru, Paul D. Bieniasz, Marina Caskey, Michel C. Nussenzweig
Summary: After infection with SARS-CoV-2, antibody levels against the spike protein decrease significantly, but the number of memory B cells remain unchanged, indicating an evolving humoral response at 6.2 months after infection.
Article
Cell Biology
Zijun Wang, Julio C. C. Lorenzi, Frauke Muecksch, Shlomo Finkin, Charlotte Viant, Christian Gaebler, Melissa Cipolla, Hans-Heinrich Hoffman, Thiago Y. Oliveira, Deena A. Oren, Victor Ramos, Lilian Nogueira, Eleftherios Michailidis, Davide F. Robbiani, Anna Gazumyan, Charles M. Rice, Theodora Hatziioannou, Paul D. Bieniasz, Marina Caskey, Michel C. Nussenzweig
Summary: The IgA response to SARS-CoV-2 was characterized in a cohort of 149 convalescent individuals after COVID-19 diagnosis. IgA responses in plasma generally correlated with IgG responses, and B cells producing IgM, IgG, and IgA were derived from common progenitor cells. Notably, IgA dimers were found to be 15 times more potent than IgA monomers against SARS-CoV-2, suggesting their potential value for protection and vaccine efficacy.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Hematology
Tomasz Sewastianik, Juerg R. Straubhaar, Jian-Jun Zhao, Mehmet K. Samur, Keith Adler, Helen E. Tanton, Vignesh Shanmugam, Omar Nadeem, Peter S. Dennis, Vinodh Pillai, Jianli Wang, Meng Jiang, Jianhong Lin, Ying Huang, Daniel Brooks, Mary Bouxsein, David M. Dorfman, Geraldine S. Pinkus, Davide F. Robbiani, Irene M. Ghobrial, Bogdan Budnik, Petr Jarolim, Nikhil C. Munshi, Kenneth C. Anderson, Ruben D. Carrasco
Summary: The deletion of miR-15a/16-1 cluster in murine GC B cells induces moderate but widespread molecular and functional changes, leading to the development of mature B-cell neoplasms resembling human extramedullary plasmacytoma and lymphoma. The low expression levels of miR-15a/16 in human primary EP, primarily associated with del(13q) loss, highlights the tumor-suppressor role of this cluster in plasma cell and B-cell malignancies.
Article
Immunology
Marianna Agudelo, Martin Palus, Jennifer R. Keeffe, Filippo Bianchini, Pavel Svoboda, Jiri Salat, Avery Peace, Anna Gazumyan, Melissa Cipolla, Tania Kapoor, Francesca Guidetti, Kai-Hui Yao, Jana Elsterova, Dana Teislerova, Ales Chrdle, Vaclav Honig, Thiago Oliveira, Anthony P. West, Yu E. Lee, Charles M. Rice, Margaret R. MacDonald, Pamela J. Bjorkman, Daniel Ruzek, Davide F. Robbiani, Michel C. Nussenzweig
Summary: The study revealed the human neutralizing antibody response to TBEV, showing that the most potent neutralizing antibodies were found in individuals who recovered from natural infection. These antibodies also neutralized other tick-borne flaviviruses, and structural analysis identified a conserved epitope near the lateral ridge of EDIII. Prophylactic or early therapeutic antibody administration was effective at low doses in mice lethally infected with TBEV.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Raoul De Gasparo, Mattia Pedotti, Luca Simonelli, Petr Nickl, Frauke Muecksch, Irene Cassaniti, Elena Percivalle, Julio C. C. Lorenzi, Federica Mazzola, Davide Magri, Tereza Michalcikova, Jan Haviernik, Vaclav Honig, Blanka Mrazkova, Natalie Polakova, Andrea Fortova, Jolana Tureckova, Veronika Iatsiuk, Salvatore Di Girolamo, Martin Palus, Dagmar Zudova, Petr Bednar, Ivana Bukova, Filippo Bianchini, Dora Mehn, Radim Nencka, Petra Strakova, Oto Pavlis, Jan Rozman, Sabrina Gioria, Jose Camilla Sammartino, Federica Giardina, Stefano Gaiarsa, Qiang Pan-Hammarstrom, Christopher O. Barnes, Pamela J. Bjorkman, Luigi Calzolai, Antonio Piralla, Fausto Baldanti, Michel C. Nussenzweig, Paul D. Bieniasz, Theodora Hatziioannou, Jan Prochazka, Radislav Sedlacek, Davide F. Robbiani, Daniel Ruzek, Luca Varani
Summary: The bispecific IgG1-like molecule CoV-X2 can simultaneously bind two independent sites on the RBD, prevent spike binding to ACE2, neutralize SARS-CoV-2 and its variants, and protect mice from disease in a mouse model of SARS-CoV-2 infection. This demonstrates the feasibility and efficacy of targeting non-overlapping RBD epitopes with IgG-like bispecific antibodies.
Editorial Material
Immunology
Davide F. Robbiani, Daniel Ruzek
Summary: The NS1 protein of flaviviruses has become a focus of research for its potential in vaccine and immunotherapeutic development. However, a recent study has revealed a potential dark side to NS1, linking it to the development of self-reactive antibodies.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Allison J. Greaney, Tyler N. Starr, Christopher O. Barnes, Yiska Weisblum, Fabian Schmidt, Marina Caskey, Christian Gaebler, Alice Cho, Marianna Agudelo, Shlomo Finkin, Zijun Wang, Daniel Poston, Frauke Muecksch, Theodora Hatziioannou, Paul D. Bieniasz, Davide F. Robbiani, Michel C. Nussenzweig, Pamela J. Bjorkman, Jesse D. Bloom
Summary: The study found that although the human immune system can produce antibodies that target diverse RBD epitopes, in practice, the polyclonal response to infection tends to be skewed towards a single class of antibodies targeting an epitope that is already undergoing rapid evolution.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Filippo Bianchini, Virginia Crivelli, Morgan E. Abernathy, Concetta Guerra, Martin Palus, Jonathan Muri, Harold Marcotte, Antonio Piralla, Mattia Pedotti, Raoul De Gasparo, Luca Simonelli, Milos Matkovic, Chiara Toscano, Maira Biggiogero, Veronica Calvaruso, Pavel Svoboda, Tomas Cervantes Rincon, Tommaso Fava, Lucie Podesvova, Akanksha A. Shanbhag, Andrea Celoria, Jacopo Sgrignani, Michal Stefanik, Vaclav Honig, Veronika Pranclova, Tereza Michalcikova, Jan Prochazka, Giuditta Guerrini, Dora Mehn, Annalisa Clabbattini, Hassan Abolhassani, David Jarrossay, Mariagrazia Uguccioni, Donata Medaglini, Qiang Pan-Hammarstroem, Luigi Calzolai, Daniel Fernandez, Fausto Baldanti, Alessandra Franzetti-Pellanda, Christian Garzoni, Radislav Sedlacek, Daniel Ruzek, Luca Varani, Andrea Cavalli, Christopher O. Barnes, Davide F. Robbiani
Summary: Using coldspot-guided antibody discovery, neutralizing antibodies to highly conserved viral epitopes were identified. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1 and synergizes with antibody rbd.042 for neutralization.
SCIENCE IMMUNOLOGY
(2023)
Article
Immunology
Jonathan Muri, Valentina Cecchinato, Andrea Cavalli, Akanksha A. A. Shanbhag, Milos Matkovic, Maira Biggiogero, Pier Andrea Maida, Jacques Moritz, Chiara Toscano, Elaheh Ghovehoud, Raffaello Furlan, Franca Barbic, Antonio Voza, Guendalina De Nadai, Carlo Cervia, Yves Zurbuchen, Patrick Taeschler, Lilly A. Murray, Gabriela Danelon-Sargenti, Simone Moro, Tao Gong, Pietro Piffaretti, Filippo Bianchini, Virginia Crivelli, Lucie Podesvova, Mattia Pedotti, David Jarrossay, Jacopo Sgrignani, Sylvia Thelen, Mario Uhr, Enos Bernasconi, Andri Rauch, Antonio Manzo, Adrian Ciurea, Marco B. L. Rocchi, Luca Varani, Bernhard Moser, Barbara Bottazzi, Marcus Thelen, Brian A. Fallon, Onur Boyman, Alberto Mantovani, Christian Garzoni, Alessandra Franzetti-Pellanda, Mariagrazia Uguccioni, Davide F. Robbiani
Summary: We found that antibodies against specific chemokines are commonly present in COVID-19 convalescents and are associated with favorable disease outcomes and a decreased risk of long COVID development at 1 year post-infection. These chemokine antibodies are also found in HIV-1 infection and autoimmune disorders, but target different chemokines compared to COVID-19. Monoclonal antibodies derived from COVID-19 convalescents that bind to the chemokine N-loop impair cell migration. Naturally occurring chemokine antibodies may modulate the inflammatory response and have therapeutic potential.