期刊
EMBO JOURNAL
卷 30, 期 18, 页码 3864-3874出版社
WILEY
DOI: 10.1038/emboj.2011.279
关键词
bacterial aggregation and biofilm formation; Haemophilus influenzae Hap adhesin; oligomerization; self-associating autotransporter; structural biology
资金
- Shanghai Institute of Higher Learning
- National Natural Science Foundation of China [31070645]
- NIH [R01 AI49322]
- MRC [G0800002] Funding Source: UKRI
- Medical Research Council [G0800002] Funding Source: researchfish
Bacterial biofilms are complex microbial communities that are common in nature and are being recognized increasingly as an important determinant of bacterial virulence. However, the structural determinants of bacterial aggregation and eventual biofilm formation have been poorly defined. In Gram-negative bacteria, a major subgroup of extracellular proteins called self-associating autotransporters (SAATs) can mediate cell-cell adhesion and facilitate biofilm formation. In this study, we used the Haemophilus influenzae Hap autotransporter as a prototype SAAT to understand how bacteria associate with each other. The crystal structure of the H. influenzae HapS passenger domain (harbouring the SAAT domain) was determined to 2.2 angstrom by X-ray crystallography, revealing an unprecedented intercellular oligomerization mechanism for cell-cell interaction. The C-terminal SAAT domain folds into a triangular-prism-like structure that can mediate Hap-Hap dimerization and higher degrees of multimerization through its F1-F2 edge and F2 face. The intercellular multimerization can give rise to massive buried surfaces that are required for overcoming the repulsive force between cells, leading to bacterial cell-cell interaction and formation of complex microcolonies. The EMBO Journal (2011) 30, 3864-3874. doi: 10.1038/emboj.2011.279; Published online 12 August 2011
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