Article
Multidisciplinary Sciences
Himanshu Pandey, Emanuel Reithmann, Alina Goldstein-Levitin, Jawdat Al-Bassam, Erwin Frey, Larisa Gheber
Summary: The study reveals clustering-induced directionality switching of the bidirectional kinesin-5 Cin8, where the motor protein is able to change direction under the influence of opposing forces. By developing a model, researchers successfully predicted and explained this mechanism, which can account for the reversal of direction in bidirectional motor proteins under different experimental conditions.
Article
Biochemistry & Molecular Biology
Himanshu Pandey, Sudhir Kumar Singh, Mayan Sadan, Mary Popov, Meenakshi Singh, Geula Davidov, Sayaka Inagaki, Jawdat Al-Bassam, Raz Zarivach, Steven S. Rosenfeld, Larisa Gheber
Summary: This study investigates two modes of motility of the bi-directional kinesin-5 motor protein Cin8 and how motor-microtubule interactions affect these modes. The findings suggest that the large insert in loop 8 (L8) within the motor domain of Cin8 plays a crucial role in enabling flexible anchoring to microtubules, allowing for both context-dependent directionality reversal and bi-directional motility modes. Furthermore, the study demonstrates that L8-induced flexible microtubule anchoring is necessary for bi-directional motility of Cin8 at the single molecule level.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Jessica J. A. Hummel, Casper C. Hoogenraad
Summary: In this study, the authors systematically investigated the selectivity and regulation of KIF1A-driven transport of different types of vesicles, emphasizing the importance of phosphorylation for vesicle binding and the role of specific adaptors in achieving cargo specificity. They propose a model where motor dimerization, posttranslational modifications, and specific adaptors regulate selective KIF1A cargo trafficking.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Byron Hunter, Matthieu P. M. H. Benoit, Ana B. Asenjo, Caitlin Doubleday, Daria Trofimova, Corey Frazer, Irsa Shoukat, Hernando Sosa, John S. Allingham
Summary: This study reveals how Kinesin-8 alternates between promotility and pro-microtubule-depolymerization states by regulating its tubulin structure through the loop-2 region. Structural changes induced by curved microtubule ends and straight protofilaments result in different functional outcomes.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Rui Jiang, William O. Hancock
Summary: The study presents a method to measure the on-rate of motors by using a modified microtubule gliding assay on a supported lipid bilayer, detecting motor binding through an increase in fluorescence.
Article
Cell Biology
Linda Balabanian, Dominique Lessard, Karthikeyan Swaminathan, Pamela Yaninska, Muriel Sebastien, Samuel Wang, Piper W. Stevens, Paul W. Wiseman, Christopher L. Berger, Adam G. Hendricks
Summary: Microtubule-associated proteins (MAPs) regulate the motility of kinesin and dynein along microtubules, controlling the transport of vesicles and organelles. Different cargoes have diverse responses to MAP tau, likely due to the types of kinesin motors driving their transport.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Review
Cell Biology
Omayma Al Azzam, Cameron Lee Trussell, Dana N. Reinemann
Summary: By combining the precision of optical tweezers with reconstituted ensemble systems of microtubules, MAPs, and kinesins, researchers have gained insights into how these unique elements work together to perform cellular tasks such as building the mitotic spindle. This approach not only developed novel techniques for studying motor proteins in vitro, but also illuminated ensemble filament and motor synergy, bridging the mechanistic knowledge gap between previous single molecule and cell level studies.
Editorial Material
Biochemistry & Molecular Biology
Marco Tarantola, Tim Meyer, Christoph F. Schmidt, Wolfram-Hubertus Zimmermann
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Raju Baskar, Anna Bahkrat, Tetsuhisa Otani, Housei Wada, Geula Davidov, Himanshu Pandey, Larisa Gheber, Raz Zarivach, Shigeo Hayashi, Uri Abdu
Article
Biochemistry & Molecular Biology
Radoslaw Katana, Chonglin Guan, Damiano Zanini, Matthew E. Larsen, Diego Giraldo, Bart R. H. Geurten, Christoph F. Schmidt, Steven G. Britt, Martin C. Goepfert
Article
Biology
Tatyana Bodrug, Elizabeth M. Wilson-Kubalek, Stanley Nithianantham, Alex F. Thompson, April Alfieri, Ignas Gaska, Jennifer Major, Garrett Debs, Sayaka Inagaki, Pedro Gutierrez, Larisa Gheber, Richard J. McKenney, Charles Vaughn Sindelar, Ronald Milligan, Jason Stumpff, Steven S. Rosenfeld, Scott T. Forth, Jawdat Al-Bassam
Article
Chemistry, Analytical
Mohammad T. Rabbani, Christoph F. Schmidt, Alexandra Ros
ANALYTICAL CHEMISTRY
(2020)
Article
Medicine, Legal
A. Lange, K. Keller, S. Lakamper
Article
Multidisciplinary Sciences
Himanshu Pandey, Emanuel Reithmann, Alina Goldstein-Levitin, Jawdat Al-Bassam, Erwin Frey, Larisa Gheber
Summary: The study reveals clustering-induced directionality switching of the bidirectional kinesin-5 Cin8, where the motor protein is able to change direction under the influence of opposing forces. By developing a model, researchers successfully predicted and explained this mechanism, which can account for the reversal of direction in bidirectional motor proteins under different experimental conditions.
Article
Chemistry, Multidisciplinary
Sufi O. Raja, Alexey Chizhik, Christoph F. Schmidt, Joerg Enderlein, Arindam Ghosh
Summary: This study used GIET imaging to observe the changes in mitochondrial membranes before and during ATP synthesis, showing that the inner membrane approaches the outer membrane while the inter-membrane space is reduced by approximately 2 nm.
Article
Biology
Alina Goldstein-Levitin, Himanshu Pandey, Kanary Allhuzaeel, Itamar Kass, Larisa Gheber
Summary: This study analyzed intracellular functions and motile properties of neck-linker variants of the bi-directional kinesin-5 motor Cin8. The results showed that exact N-latch stabilization during NL docking is critical for the function of bi-directional kinesin-5 Cin8.
Article
Physics, Multidisciplinary
Kengo Nishi, Fred C. MacKintosh, Christoph F. Schmidt
Summary: The mechanical properties of soft materials can be probed on small length scales by filament-based microrheology using high-aspect-ratio semiflexible filaments as less invasive probes. By imaging transverse bending modes, the micromechanical response of the medium can be simultaneously determined on multiple length scales. The use of semiflexible single-walled carbon nanotubes as probes allows for accurate and rapid imaging with stable near-IR fluorescence, providing results in good agreement with conventional micro- and macrorheology measurements of viscoelastic properties of solutions such as sucrose, polyethylene oxide, and hyaluronic acid.
PHYSICAL REVIEW LETTERS
(2021)
Article
Developmental Biology
Daniel Haertter, Xiaolei Wang, Stephanie M. Fogerson, Nitya Ramkumar, Janice M. Crawford, Kenneth D. Poss, Stefano Di Talia, Daniel P. Kiehart, Christoph F. Schmidt
Summary: This study presents DeepProjection, a trainable projection algorithm based on deep learning, for efficient extraction of image data from curved tissue sheets in volumetric imaging data. The algorithm can predict binary masks containing the target content and generate background-free 2D images. It can also be applied to other fluorescent channels or extract tissue curvature.
Article
Medicine, Research & Experimental
Michael Scholz, Stefan Lakaemper, Kristina Keller, Akos Dobay, Andrea Eva Steuer, Hans-Peter Landolt, Thomas Kraemer
Summary: This study aims to identify the impact of insufficient sleep on metabolism and search for biomarkers of sleepiness. By observing changes in metabolic profiles and behavioral outcomes under different sleep-wake schedules, this is the first trial to comprehensively investigate the combination of metabolic profiles and performance monitoring. The findings of this study will have significant value for various related disciplines.
Article
Nanoscience & Nanotechnology
Christina Jayachandran, Arindam Ghosh, Meenakshi Prabhune, Jonathan Bath, Andrew J. J. Turberfield, Lara Hauke, Jorg Enderlein, Florian Rehfeldt, Christoph F. F. Schmidt
Summary: Mechanical forces play a critical role in various biological processes, including wound healing, tumor formation, cell migration, and differentiation. This study presents a FRET-based DNA-protein tension sensor designed to measure transient forces in actin networks. The sensor utilizes two actin-binding motifs with a fast off-rate attached to a central DNA hairpin loop. The conformational state of the sensor is determined using fluorescence lifetime imaging, enabling robust measurements even with intensity variations. The sensor's potential is demonstrated through confocal microscopy and monitoring of crosslinking activity in vitro using bulk rheology.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Physical
D. Mizuno, C. Tardin, C. F. Schmidt
Article
Nanoscience & Nanotechnology
Eugenia Butkevich, Navneet C. Verma, Nazar Oleksiievets, Ingo Gregor, Christoph F. Schmidt, Joerg Enderlein, Chayan K. Nandi, Alexey I. Chizhik
ACS APPLIED NANO MATERIALS
(2019)