Article
Cell Biology
Sophie J. F. van der Spek, Nikhil J. Pandya, Frank Koopmans, Iryna Paliukhovich, Roel C. van der Schors, Mylene Otten, August B. Smit, Ka Wan Li
Summary: AMPA glutamate receptor (AMPA-R) is the major excitatory ionotropic receptor at synapses in the brain. Different subunits and subtypes of AMPA receptors have different effects on synaptic plasticity and interact with various associated proteins. However, there is a lack of systematic analysis on the specific interactome of AMPA receptor subtypes.
Article
Biochemistry & Molecular Biology
Preeti Dhaka, Ankur Singh, Shweta Choudhary, Rama Krishna Peddinti, Pravindra Kumar, Gaurav Kumar Sharma, Shailly Tomar
Summary: This study identifies potential antiviral molecules against SARS-CoV-2 by targeting the RNA-binding site in the N-terminal domain of the nucleocapsid protein. Twelve high-affinity molecules that bind to the RNA binding site are identified using a structure-based repurposing approach. Two compounds, Telmisartan and BMS-189453, show potential antiviral activity against SARS-CoV-2.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Diego Cantoni, Matthew J. Murray, Mphatso D. Kalemera, Samuel J. Dicken, Lenka Stejskal, Georgina Brown, Spyros Lytras, Jonathon D. Coey, James McKenna, Stephen Bridgett, David Simpson, Derek Fairley, Lucy G. Thorne, Ann-Kathrin Reuschl, Calum Forrest, Maaroothen Ganeshalingham, Luke Muir, Machaela Palor, Lisa Jarvis, Brian Willett, Ultan F. Power, Laura E. McCoy, Clare Jolly, Greg J. Towers, Katie J. Doores, David L. Robertson, Adrian J. Shepherd, Matthew B. Reeves, Connor G. G. Bamford, Joe Grove
Summary: The emergence of SARS-CoV-2 variants with mutations in the spike glycoprotein, particularly in the N-terminal domain (NTD) flexible loops, has significant implications for viral transmission and immune response. Variations in NTD loop length can impact virus entry and may provide a mechanism for the virus to adapt and optimize its functionality in a complex selection environment.
Article
Biology
Fatemeh Bayani, Negin Safaei Hashkavaei, Vladimir N. Uversky, Sina Mozaffari-Jovin, Yahya Sefidbakht
Summary: This study investigated the impact of mutations in the NTD and RBD domains of the spike protein on the development of COVID-19 vaccines using computational analysis. The results showed that long glycans can reduce the accessibility of protein epitopes, thereby affecting the binding efficiency and neutralizing potency of specific antibodies. Additionally, the presence of long glycans enhanced the stability and affinity of the RBD to ACE2. The study also identified key residues that play a role in increasing the structural stability of the protein. These findings contribute to a better understanding of the structural changes in the spike protein and have implications for the development of novel therapeutics.
COMPUTERS IN BIOLOGY AND MEDICINE
(2022)
Article
Immunology
Huibin Lv, Owen Tak-Yin Tsang, Ray T. Y. So, Yiquan Wang, Meng Yuan, Hejun Liu, Garrick K. Yip, Qi Wen Teo, Yihan Lin, Weiwen Liang, Jinlin Wang, Wilson W. Ng, Ian A. Wilson, J. S. Malik Peiris, Nicholas C. Wu, Chris K. P. Mok
Summary: Specific antibodies against SARS-CoV-2 NTD can be induced during infection, but show less cross-reactivity with the 2003 pandemic strain SARS-CoV. When used as an immunogen, NTD rarely induces neutralizing antibodies, suggesting it may not be suitable for vaccine development.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anja Goeder, Torsten Ginter, Thorsten Heinzel, Svenja Stroh, Joerg Fahrer, Andreas Henke, Oliver H. Kraemer
Summary: The N-terminal domain (NTD) of STAT1 plays a crucial role in IFN-induced signaling, affecting the phosphorylation, nuclear translocation, and gene expression regulation of STAT1.
Article
Multidisciplinary Sciences
Chao-Hua Jiang, Mengping Wei, Chen Zhang, Yun Stone Shi
Summary: Long-term potentiation (LTP) is a crucial cellular mechanism for learning and memory, with the amino-terminal domain (ATD) of GluA1 interacting with neuroplastin-65 (Np65) playing a key role in anchoring AMPARs at the postsynaptic membrane during LTP.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Jiale Shi, Yuejun Shi, Ruixue Xiu, Gang Wang, Rui Liang, Yuzhou Jiao, Zhou Shen, Chengliang Zhu, Guiqing Peng
Summary: This study identified a neutralizing epitope on the N-terminal domain (NTD) of the alphacoronavirus HCoV-229E spike protein and characterized a neutralizing antibody targeting this epitope. The study also revealed that the epitopes on the NTD can change over time, providing a theoretical basis for the prevention and control of HCoV-229E or related coronaviruses.
JOURNAL OF VIROLOGY
(2022)
Article
Cell Biology
Shoji F. Kenji, Keerthi Kurma, Brigitte Collet, Christelle Oblet, Laure Debure, Carmelo Di Primo, Laetitia Minder, Franck Verite, Yannic Danger, Mickael Jean, Aubin Penna, Nicolas Levoin, Patrick Legembre
Summary: CD95 is a death receptor that promotes oncogenesis through various molecular mechanisms. This receptor can undergo cleavage and modification in both cancer cells and normal cells, with the modified N-terminal region potentially regulating receptor aggregation and death signaling.
CELL DEATH & DISEASE
(2022)
Article
Virology
Hua Guo, Ang Li, Hao-Feng Lin, Mei-Qin Liu, Jing Chen, Ting-Ting Jiang, Bei Li, Yi Wang, Michael C. Letko, Wenjie Peng, Zheng-Li Shi
Summary: The N-terminal domains of sarbecoviruses are highly diverse, and their function is not well studied compared to the receptor-binding domains. This study demonstrates that the N-terminal domains of sarbecovirus can be divided into five clades and show varying levels of glycan binding. The researchers also found that sarbecoviruses interact with cell surface glycans and that the presence of sialic acids on human lung cells inhibits sarbecovirus infection, which is different from other respiratory coronaviruses.
JOURNAL OF VIROLOGY
(2022)
Article
Neurosciences
Ruiting Li, Ruhar Singh, Tara Kashav, ChunMin Yang, Ravi Datta Sharma, Andrew M. Lynn, Rajendra Prasad, Amresh Prakash, Vijay Kumar
Summary: This study investigated the folding and unfolding of the N-terminal domain (NTD) of TDP-43 protein at high temperatures using molecular dynamics simulations. Results showed that the unfolding process of NTD at high temperature involved the formation of different conformational states with varying stability and free energy. The presence of structurally heterogeneous intermediate ensembles during unfolding may facilitate NTD oligomerization and subsequent TDP-43 oligomerization.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Longying Jiang, Huajun Zhang, Desheng Xiao, Hudie Wei, Yongheng Chen
Summary: FXR is a crucial bile acid activated nuclear receptor that regulates key genes involved in bile acid metabolism. With the approval of OCA as the first small molecule targeting FXR, many other FXR-targeting small molecules are currently being evaluated in clinical trials.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Review
Neurosciences
Mark L. Mayer
Summary: This review summarizes structural studies on kainate receptors, explaining their unique functional properties. Insights into the key role mechanisms in assembly of kainate receptors have been revealed through various structures and biochemical studies. Additionally, novel structures for the desensitized state of homomeric and heteromeric kainate receptors have been discovered, differing significantly from AMPA receptors.
Article
Biochemistry & Molecular Biology
Naveenchandra Suryadevara, Swathi Shrihari, Pavlo Gilchuk, Laura A. VanBlargan, Elad Binshtein, Seth J. Zost, Rachel S. Nargi, Rachel E. Sutton, Emma S. Winkler, Elaine C. Chen, Mallorie E. Fouch, Edgar Davidson, Benjamin J. Doranz, Rita E. Chen, Pei-Yong Shi, Robert H. Carnahan, Larissa B. Thackray, Michael S. Diamond, James E. Crowe
Summary: The study found that a subset of human monoclonal antibodies derived from convalescent SARS-CoV-2 patients possess neutralizing activity, with two antibodies capable of inhibiting infection. Mechanistic studies revealed these antibodies neutralize in part by inhibiting post-attachment steps in the infection cycle.
Article
Microbiology
Denise Haslwanter, M. Eugenia Dieterle, Anna Z. Wec, Cecilia M. O'Brien, Mrunal Sakharkar, Catalina Florez, Karen Tong, C. Garrett Rappazzo, Gorka Lasso, Olivia Vergnolle, Ariel S. Wirchnianski, Robert H. Bortz, Ethan Laudermilch, J. Maximilian Fels, Amanda Mengotto, Ryan J. Malonis, George Georgiev, Jose A. Quiroz, Daniel Wrapp, Nianshuang Wang, Kathryn E. Dye, Jason Barnhill, John M. Dye, Jason S. McLellan, Johanna P. Daily, Jonathan R. Lai, Andrew S. Herbert, Laura M. Walker, Kartik Chandran, Rohit K. Jangra
Summary: The study identified a panel of potent RBD and NTD nAbs against SARS-CoV-2, with the most potent RBD nAb targeting a residue at F490 and the most potent NTD nAb epitope including Y145, K150, and W152. Combining RBD and NTD nAbs did not enhance neutralization potential but limited the development of neutralization-escape mutants in vitro, suggesting a potential strategy to mitigate the emergence of VOC.
Article
Chemistry, Multidisciplinary
Yu Heng Lau, Yuteng Wu, Maxim Rossmann, Ban Xiong Tan, Peterson de Andrade, Yaw Sing Tan, Chandra Verma, Grahame J. McKenzie, Ashok R. Venkitaraman, Marko Hyvoenen, David R. Spring
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2015)
Review
Biochemical Research Methods
Gerhard Fischer, Maxim Rossmann, Marko Hyvoenen
CURRENT OPINION IN BIOTECHNOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Yuteng Wu, Lasse B. Olsen, Yu Heng Lau, Claus Hatt Jensen, Maxim Rossmann, Ysobel R. Baker, Hannah F. Sore, Suil Collins, David R. Spring
Article
Multidisciplinary Sciences
Jonathan Elegheert, Wataru Kakegawa, Jordan E. Clay, Natalie F. Shanks, Ester Behiels, Keiko Matsuda, Kazuhisa Kohda, Eriko Miura, Maxim Rossmann, Nikolaos Mitakidis, Junko Motohashi, Veronica T. Chang, Christian Siebold, Ingo H. Greger, Terunaga Nakagawa, Michisuke Yuzaki, A. Radu Aricescu
Article
Biochemistry & Molecular Biology
Maxim Rossmann, Sandra J. Greive, Tommaso Moschetti, Michael Dinan, Marko Hyvonen
PROTEIN ENGINEERING DESIGN & SELECTION
(2017)
Article
Biochemistry & Molecular Biology
Maxim Rossmann, Madhav Sukumaran, Andrew C. Penn, Dmitry B. Veprintsev, M. Madan Babu, Ingo H. Greger
Article
Biochemistry & Molecular Biology
Alessandra Beltrami, Maxim Rossmann, Maria Teresa Fiorillo, Fabiana Paladini, Rosa Sorrentino, Wolfram Saenger, Pravin Kumar, Andreas Ziegler, Barbara Uchanska-Ziegler
JOURNAL OF BIOLOGICAL CHEMISTRY
(2008)
Article
Biochemistry & Molecular Biology
Daria Slowik, Maxim Rossmann, Petr V. Konarev, Klaus-D Irrgang, Wolfram Saenger
JOURNAL OF MOLECULAR BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Maxim Rossmann, Robert Schultz-Heienbrok, Joachim Behlke, Natascha Remmel, Claudia Alings, Konrad Sandhoff, Wolfram Saenger, Timm Maier
Article
Chemistry, Multidisciplinary
Yu Heng Lau, Peterson de Andrade, Soo-Tng Quah, Maxim Rossmann, Luca Laraia, Niklas Skoeld, Tze Jing Sum, Pamela J. E. Rowling, Thomas L. Joseph, Chandra Verma, Marko Hyvoenen, Laura S. Itzhaki, Ashok R. Venkitaraman, Christopher J. Brown, David P. Lane, David R. Spring
Article
Multidisciplinary Sciences
Matej Janecek, Maxim Rossmann, Pooja Sharma, Amy Emery, David J. Huggins, Simon R. Stockwell, Jamie E. Stokes, Yaw S. Tan, Estrella Guarino Almeida, Bryn Hardwick, Ana J. Narvaez, Marko Hyvonen, David R. Spring, Grahame J. McKenzie, Ashok R. Venkitaraman
SCIENTIFIC REPORTS
(2016)
Article
Chemistry, Multidisciplinary
Daniel J. Cole, Matej Janecek, Jamie E. Stokes, Maxim Rossmann, John C. Faver, Grahame J. McKenzie, Ashok R. Venkitaraman, Marko Hyvonen, David R. Spring, David J. Huggins, William L. Jorgensen
CHEMICAL COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Vladimir Zaichick, Sofia Zaichick, Maxim Rossmann
AIMS MOLECULAR SCIENCE
(2016)
Article
Biochemistry & Molecular Biology
Silvia Locatelli-Hoops, Natascha Remmel, Ralf Klingenstein, Bernadette Breiden, Maksim Rossocha, Maike Schoeniger, Christine Koenigs, Wolfram Saenger, Konrad Sandhoff
JOURNAL OF BIOLOGICAL CHEMISTRY
(2006)