Article
Biochemistry & Molecular Biology
Marin Barisic, Girish Rajendraprasad
Summary: Recent research has shown that in human cells, the continuous poleward motion of microtubules in mitotic spindles is driven by four mitotic kinesins, with specific contributions at different stages of mitosis. While the molecular mechanism behind this cellular phenomenon has been elucidated, the functional roles of microtubule poleward flux during cell division remain largely unknown.
Review
Cell Biology
Angela R. R. Bunning, Mohan L. L. Gupta Jr.
Summary: Accurate chromosome segregation is essential for cell and organismal viability. The mitotic spindle, composed mainly of dynamic microtubules, plays a crucial role in this process by forming a bipolar configuration and generating tension across sister kinetochores. Recent advances have shed light on the role of tension as a biophysical signal that ensures accurate chromosome segregation by destabilizing aberrant kinetochore-microtubule attachments and reinforcing correct connections.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soumitra Polley, Helen Mueschenborn, Melina Terbeck, Anna De Antoni, Ingrid R. Vetter, Marileen Dogterom, Andrea Musacchio, Vladimir A. Volkov, Pim J. Huis In 't Veld
Summary: During cell division, the Ndc80 complex plays a crucial role in linking chromosomes to spindle microtubules. This study demonstrates that the Ndc80 loop promotes direct interactions between adjacent Ndc80 complexes, which are necessary for stable end-on kinetochore-microtubule attachment and spindle assembly checkpoint satisfaction. Mutations in the loop impair these interactions and lead to cell arrest in mitosis.
Review
Cell Biology
Masamitsu Sato, Yasutaka Kakui, Mika Toya
Summary: Meiosis is a specialized cell division process for gamete production in eukaryotes, with chromosomes and microtubules mutually influencing each other. Studies on the fission yeast Schizosaccharomyces pombe have revealed the organization of chromosomes, cytoskeleton, and cell cycle progression in mitosis and meiosis. Meiosis is strategically designed for faithful genetic material segregation and genetic diversity production through meiosis-specific and general factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Janet K. Jang, Amy C. Gladstein, Arunika Das, Joanatta G. Shapiro, Zachary L. Sisco, Kim S. McKim
Summary: The study highlights the critical role of PP2A in maintaining spindle assembly and sister chromatid cohesion in Drosophila oocytes, showing an interplay with Aurora B activity.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biology
William Conway, Robert Kiewisz, Gunar Fabig, Colm P. Kelleher, Hai-Yin Wu, Maya Anjur-Dietrich, Thomas Mueller-Reichert, Daniel J. Needleman
Summary: During eukaryotic cell division, the distribution of kinetochore microtubules (KMTs) in the mitotic spindle is crucial for accurate chromosome segregation. This study investigates the processes that give rise to the observed distribution of KMTs in human cells. The results suggest that KMTs grow away from kinetochores along defined trajectories, with longer KMTs growing more slowly than shorter ones, leading to a mathematical model of kinetochore-fiber self-organization in human mitotic spindles.
Review
Genetics & Heredity
Andrew D. McAinsh, Adele L. Marston
Summary: This paper presents the components, assembly, structure, and function of kinetochores. The authors outline a blueprint for kinetochore assembly and discuss how functions are mapped onto this architecture. They also explore how the underlying chromatin influences kinetochore shape.
ANNUAL REVIEW OF GENETICS
(2022)
Review
Cell Biology
Joao Barbosa, Claudio E. Sunkel, Carlos Conde
Summary: This review provides an overview of the molecular strategies that monitor and fine-tune KT-MT attachment formation during mitosis, to ensure accurate segregation of sister chromatids. This process is crucial for safe mitotic progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Olivera Mitevska, Pak Wing Lam, Lydia Daly, Philip Auckland
Summary: Mitotic cell division requires the formation of microtubule attachments for chromosome segregation and mitotic progression. The shedding of the fibrous corona, which is mediated by dynein, plays a crucial role in this process. Little is known about the regulation of dynein stripping and its response to attachment maturation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Benjamin Craske, Thibault Legal, Julie P. I. Welburn
Summary: In this study, we characterized the motility of human CENP-E in vitro and found that the full-length form exhibits longer run length and residency times on microtubules compared to motor truncations. We also discovered that the non-motor coiled-coil regions self-regulate the motor activity. These findings provide a useful tool to study the mechanistic basis of how human CENP-E drives chromosome congression and spindle organization.
Review
Cell Biology
Shreyas Sridhar, Tatsuo Fukagawa
Summary: The assembly of functional kinetochore is crucial for accurate chromosome segregation during mitosis. Internal and external structures of kinetochore face multiple challenges, but linker proteins play an important role in force propagation and recruitment of outer kinetochore. Understanding these linkages and regulatory pathways is essential for understanding kinetochore structural diversity and plasticity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Yuqing Zhang, Chunlin Song, Lei Wang, Hongfei Jiang, Yujing Zhai, Ying Wang, Jing Fang, Gang Zhang
Summary: This review summarizes the research progress on the role of Bub1 in mitosis over the past three decades. The studies have revealed that Bub1 plays important roles in checkpoint regulation and chromosome alignment. The essentiality of Bub1 in higher eukaryotic cells is discussed, along with some unresolved questions raised for future study.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Marco A. Andonegui-Elguera, Rodrigo E. Caceres-Gutierrez, Alejandro Lopez-Saavedra, Fernanda Cisneros-Soberanis, Montserrat Justo-Garrido, Jose Diaz-Chavez, Luis A. Herrera
Summary: During mitosis, chromatin undergoes various activities regulated by histone post-translational modifications (HPTMs). Specific HPTMs play roles in chromosome condensation, centromere formation, and the assembly of regulatory protein complexes. Additionally, environmental factors can modify HPTMs, affecting chromosome segregation and genome stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Pablo Lara-Gonzalez, Jonathon Pines, Arshad Desai
Summary: The mitotic spindle is a macromolecular machine responsible for faithful chromosome segregation during cell division. Assembly of the spindle is guided by the 'Search & Capture' principle in structured space. Nucleation of noncentrosomal microtubules near kinetochores and changes in kinetochore architecture promote rapid and accurate assembly of the spindle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Aamir Ali, P. Todd Stukenberg
Summary: Cell division events are regulated to occur in a distinct order. The traditional view is that events are ordered based on changes in Cyclin Dependent Kinase (CDK) activities. However, recent studies suggest that events are actually ordered based on the position of chromatids during anaphase. This positioning is regulated by a gradient of Aurora B kinase activity and Aurora A kinase activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Norihiko Kobayashi, Yutaka Suzuki, Lori W. Schoenfeld, Carolin A. Mueller, Conrad Nieduszynski, Kenneth H. Wolfe, Tomoyuki U. Tanakal
Article
Cell Biology
Maria Kalantzaki, Etsushi Kitamura, Tongli Zhang, Akihisa Mino, Bele Novak, Tomoyuki U. Tanaka
NATURE CELL BIOLOGY
(2015)
Correction
Cell Biology
Maria Kalantzaki, Etsushi Kitamura, Tongli Zhang, Akihisa Mino, Bela Novak, Tomoyuki U. Tanaka
NATURE CELL BIOLOGY
(2015)
Article
Cell Biology
David Dickerson, Marek Gierlinski, Vijender Singh, Etsushi Kitamura, Graeme Ball, Tomoyuki U. Tanaka, Tom Owen-Hughes
Article
Cell Biology
Vanya Vasileva, Marek Gierlinski, Zuojun Yue, Nicola O'Reilly, Etsushi Kitamura, Tomoyuki U. Tanaka
JOURNAL OF CELL BIOLOGY
(2017)
Article
Cell Biology
Zuojun Yue, Shinya Komoto, Marek Gierlinski, Debora Pasquali, Etsushi Kitamura, Tomoyuki U. Tanaka
JOURNAL OF CELL SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Shuyu Li, Zuojun Yue, Tomoyuki U. Tanaka
Review
Biochemistry & Molecular Biology
Tomoyuki U. Tanaka, Lesley Clayton, Toyoaki Natsume
Article
Cell Biology
Nazan Saner, Jens Karschau, Toyoaki Natsume, Marek Gierlinski, Renata Retkute, Michelle Hawkins, Conrad A. Nieduszynski, J. Julian Blow, Alessandro P. S. de Moura, Tomoyuki U. Tanaka
JOURNAL OF CELL BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Toyoaki Natsume, Carolin A. Mueller, Yuki Katou, Renata Retkute, Marek Gierlinski, Hiroyuki Araki, J. Julian Blow, Katsuhiko Shirahige, Conrad A. Nieduszynski, Tomoyuki U. Tanaka
Article
Cell Biology
John K. Eykelenboom, Marek Gierlinski, Zuojun Yue, Nadia Hegarat, Hilary Pollard, Tatsuo Fukagawa, Helfrid Hochegger, Tomoyuki U. Tanaka
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biology
Alexander J. R. Booth, Zuojun Yue, John K. Eykelenboom, Tom Stiff, G. W. Gant Luxton, Helfrid Hochegger, Tomoyuki U. Tanaka
Review
Cell Biology
John K. Eykelenboom, Tomoyuki U. Tanaka
Article
Cell Biology
Harinath Doodhi, Taciana Kasciukovic, Lesley Clayton, Tomoyuki U. Tanaka
Summary: This study reconstructed the in vitro interface of budding yeast kinetochore-microtubule interactions, showing that Aurora B kinase weakens end-on attachments by phosphorylating Dam1, promoting the exchange to a new lateral attachment for error correction.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Cell Biology
Tomoyuki U. Tanaka, Tongli Zhang
Summary: This article discusses how eukaryotic cells ensure correct chromosome segregation during mitosis, focusing on the mechanisms of error correction through the SWAP, SWITCH, and STABILIZE processes. Key players in this process include Aurora B kinase, Mps1 kinase, and Stu2 microtubule polymerase. The study mainly examines mechanisms in budding yeast, where error correction mechanisms are relatively simpler due to a single microtubule attachment at biorientation.