期刊
EMBO JOURNAL
卷 30, 期 2, 页码 427-438出版社
WILEY
DOI: 10.1038/emboj.2010.320
关键词
Importin13; karyopherin; nuclear import; Ubc9-SUMO; X-ray crystallography
资金
- Max Planck Gesellshaft
- DFG [BO3588/1-1]
Importin13 (Imp13) is an unusual beta-karyopherin that is able to both import and export cargoes in and out of the nucleus. In the cytoplasm, Imp13 associates with different cargoes such as Mago-Y14 and Ubc9, and facilitates their import into the nucleus where RanGTP binding promotes the release of the cargo. In this study, we present the 2.8 angstrom resolution crystal structure of Imp13 in complex with the SUMO E2-conjugating enzyme, Ubc9. The structure shows an uncommon mode of cargo-karyopherin recognition with Ubc9 binding at the N-terminal portion of Imp13, occupying the entire RanGTP-binding site. Comparison of the Imp13-Ubc9 complex with Imp13-Mago-Y14 shows the remarkable plasticity of Imp13, whose conformation changes from a closed ring to an open superhelix when bound to the two different cargoes. The structure also shows that the binding mode is compatible with the sumoylated states of Ubc9. Indeed, we find that Imp13 is able to bind sumoylated Ubc9 in vitro and suppresses autosumoylation activity in the complex. The EMBO Journal (2011) 30, 427-438. doi:10.1038/emboj.2010.320; Published online 7 December 2010
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据