Review
Biochemistry & Molecular Biology
John M. Ryniawec, Gregory C. Rogers
Summary: Centrosome instability is closely associated with diseases such as cancer, highlighting the importance of understanding the wide range of centrosome functions for inspiring new discoveries.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biology
Zhenguang Ying, Kaifang Wang, Junfeng Wu, Mingyu Wang, Jing Yang, Xia Wang, Guowei Zhou, Haibin Chen, Hongwu Xu, Stephen Cho Wing Sze, Feng Gao, Chunman Li, Ou Sha
Summary: This study found that HCR is localized and stabilized at the centrosome by directly binding to astrin. Both HCR and astrin play key roles in maintaining normal microtubule assembly and genomic stability.
Article
Biochemistry & Molecular Biology
Peter Tatrai, Fanni Gergely
Summary: This study shows the critical role of CDK5RAP2 and centrosomes in spindle formation during blood production. Lack of CDK5RAP2 leads to defective erythroid differentiation and the emergence of macrocytic anemia. The study suggests that disruption of centrosome and spindle function could contribute to the development of macrocytic anemias.
Article
Cell Biology
Akiko Okada, Shigehiko Yumura
Summary: Accurate placement of the cleavage furrow is crucial for successful cell division. Dictyostelium cells were used to validate previous models, and it was found that the rotational movements of the mitotic spindles stopped when the spindle elongated. Astral microtubules reaching and fixing the spindle axis caused cell elongation and division along the spindle axis.
Article
Biochemistry & Molecular Biology
Grace Y. Liu, Shiau-Chi Chen, Gang-Hui Lee, Kritika Shaiv, Pin-Yu Chen, Hsuan Cheng, Shi-Rong Hong, Wen-Ting Yang, Shih-Han Huang, Ya-Chu Chang, Hsien-Chu Wang, Ching-Lin Kao, Pin-Chiao Sun, Ming-Hong Chao, Yian-Ying Lee, Ming-Jer Tang, Yu-Chun Lin
Summary: Microtubules, a crucial cellular structure, have been studied using chemo and optogenetics methods to disassemble specific types of microtubules. The results provide insights into their roles in cellular trafficking, organelle reorganization, and cell stiffness.
Article
Physiology
Zhen Chen, Yating Xu, Dupeng Ma, Changrong Li, Ziqi Yu, Cong Liu, Tingyu Jin, Ziye Du, Zejia Li, Qi Sun, Yumin Xu, Rong Liu, Yuerong Wu, Mengcheng Luo
Summary: Cep72 is a critical component of the centrosome, participating in regulating sperm morphology, but it is dispensable for fertility in mice.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Daniela Londono-Vasquez, Alexander Jurkevich, Ahmed Z. Balboula
Summary: Here, a multi-photon laser ablation method is described to selectively deplete endogenously labeled mcMTOCs in oocytes collected from Cep192-eGfp reporter mice. This method contributes to the understanding of the molecular mechanisms underlying spindle positioning and migration in mammalian oocytes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biology
Thomas S. McAlear, Susanne Bechstedt
Summary: This study reveals that CKAP2 is the most potent microtubule growth factor, as it strongly affects nucleation and growth rates of microtubules, and suppresses catastrophes. These findings are crucial for understanding the important role of CKAP2 in cell division.
Article
Biochemistry & Molecular Biology
Anne Nehlig, Cynthia Seiler, Yulia Steblyanko, Florent Dingli, Guillaume Arras, Damarys Loew, Julie Welburn, Claude Prigent, Marin Barisic, Clara Nahmias
Summary: Depletion of microtubule-associated protein ATIP3 reduces metaphase spindle length by interacting with Kif2A and its partner Dda3 in an Aurora kinase A-dependent manner. The absence of ATIP3 leads to the accumulation of Kif2A and Dda3 at spindle poles, affecting poleward microtubule flux and spindle shortening. ATIP3 maintains Aurora A activity on the poles to control Kif2A targeting and spindle size, highlighting their mutual regulation in controlling spindle length.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Fabiola Mascanzoni, Roberta Iannitti, Antonino Colanzi
Summary: The dynamic association between the Golgi complex and the centrosome plays a crucial role in cellular processes such as cell polarization and division.
Article
Oncology
Girish Rajendraprasad, Susana Eibes, Claudia Guasch Boldu, Marin Barisic
Summary: TH588 has been identified as a promising anti-cancer compound that stabilizes microtubules in mitotic cells, leading to mitotic arrest or cell death. Its anticancer properties largely depend on its ability to target microtubules.
Article
Biochemistry & Molecular Biology
Endri Karaj, Samkeliso Dlamini, Radhika Koranne, Shaimaa H. Sindi, Lalith Perera, William R. Taylor, L. M. Viranga Tillekeratne
Summary: This study reports a new class of imidazole-based chalcones as potential antimitotic agents and investigates their structure-activity relationship. The second-generation analogs in this class show superior bioactivity and exhibit a privileged pharmacological pharmacophore of chalcones. These compounds have unique mechanisms of cytotoxic activity.
BIOORGANIC CHEMISTRY
(2022)
Article
Oncology
Jose Thaiparambil, Chandra S. Amara, Subrata Sen, Nagireddy Putluri, Randa El-Zein
Summary: The exposure to cigarette smoke can induce centrosome amplification and clustering in lung epithelial cells, which may contribute to lung carcinogenesis. The cyclin D2-mediated centrosome clustering pathway is critical for mitosis and its inhibition can lead to cell death.
Article
Cell Biology
Takashi Hotta, Yuh-Ru Julie Lee, Takumi Higaki, Takashi Hashimoto, Bo Liu
Summary: Plant cells form acentrosomal spindles with microtubules converged toward two structurally undefined poles by employing MT minus end-directed Kinesin-14 motors. The functionally redundant Kinesin-14A motors ATK1 and ATK5 form oligomeric motor complexes that translocate microtubules toward the spindle pole to establish spindles with convergent poles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Ramiro Tomasina, Fabiana C. Gonzalez, Erica S. Martins-Duarte, Philippe Bastin, Mathieu Gissot, Maria E. Francia
Summary: Centrosomes are crucial for cell division and formation of the mitotic spindle. This study reveals the important role of the inner core protein TgCep250L1 in Toxoplasma gondii cell division, as its absence leads to nuclear segregation defects and failure to assemble the mitotic spindle.
Article
Biochemistry & Molecular Biology
Tsubasa Ohashi, Tadashi Yamamoto, Yuji Yamanashi, Miho Ohsugi
Article
Cell Biology
Shou Soeda, Kaori Yamada-Nomoto, Miho Ohsugi
JOURNAL OF CELL SCIENCE
(2016)
Article
Multidisciplinary Sciences
Keishi Shintomi, Fukashi Inoue, Hiroshi Watanabe, Keita Ohsumi, Miho Ohsugi, Tatsuya Hirano
Article
Biochemistry & Molecular Biology
Akinori Takahashi, Chisato Kikuguchi, Masahiro Morita, Tetsuhiro Shimodaira, Noriko Tokai-Nishizumi, Kazumasa Yokoyama, Miho Ohsugi, Toru Suzuki, Tadashi Yamamoto
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2012)
Correction
Biochemistry & Molecular Biology
Tomoya S. Kitajima, Miho Ohsugi, Jan Ellenberg
Article
Biochemistry & Molecular Biology
Tomoya S. Kitajima, Miho Ohsugi, Jan Ellenberg
Article
Cell Biology
Natsuko Masuda, Tetsuhiro Shimodaira, Shu-Jen Shiu, Noriko Tokai-Nishizumi, Tadashi Yamamoto, Miho Ohsugi
CELL STRUCTURE AND FUNCTION
(2011)
Article
Cell Biology
Sung-Joon Park, Makiko Komata, Fukashi Inoue, Kaori Yamada, Kenta Nakai, Miho Ohsugi, Katsuhiko Shirahige
GENES & DEVELOPMENT
(2013)
Article
Cell Biology
Shou Soeda, Kaori Yamada, Miho Ohsugi
Article
Biochemistry & Molecular Biology
Yuko Ozaki, Hirotaka Matsui, Hiroya Asou, Akiko Nagamachi, Daisuke Aki, Hiroaki Honda, Shin'ichiro Yasunaga, Yoshihiro Takihara, Tadashi Yamamoto, Shunsuke Izumi, Miho Ohsugi, Toshiya Inaba
Article
Biochemistry & Molecular Biology
Sung-Joon Park, Katsuhiko Shirahige, Miho Ohsugi, Kenta Nakai
NUCLEIC ACIDS RESEARCH
(2015)
Article
Cell Biology
Shou Soeda, Kaori Yamada-Nomoto, Tatsuo Michiue, Miho Ohsugi
DEVELOPMENTAL CELL
(2018)
Article
Multidisciplinary Sciences
Takaya Totsuka, Miho Ohsugi
SCIENTIFIC REPORTS
(2020)
Meeting Abstract
Cell Biology
N. Tokai-Nishizumi, M. Ohsugi, T. Yamamoto
MOLECULAR BIOLOGY OF THE CELL
(2011)