Article
Biodiversity Conservation
Jeannette Kanefsky, Seth Smith, Kim T. Scribner
Summary: This study presents a melt curve analysis method for sex identification in lake sturgeon using qPCR assays, which is highly accurate and easy to use. The method allows rapid determination of sex in individuals of this threatened species for conservation efforts and research.
Article
Multidisciplinary Sciences
Jennifer J. Labisch, G. Philip Wiese, Kalpana Barnes, Franziska Bollmann, Karl Pflanz
Summary: The study developed a real-time imaging method for quantifying the infectious titer of lentiviral vectors, which showed lower standard deviation and a broader linear range compared to traditional methods, enabling real-time titer results.
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Health Care Sciences & Services
Mazen A. A. Atiq, Sandra E. E. Peterson, Loralie J. J. Langman, Linnea M. M. Baudhuin, John L. L. Black, Ann M. M. Moyer
Summary: CYP2D6 duplication has pharmacogenomic implications and the duplicated allele can be reliably determined using real-time PCR-based genotyping with copy number variation (CNV) detection.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Veterinary Sciences
Ciro D. Cordeiro, Nesim Gonceer, Steve Dorus, James E. Crill, Vardit Moshayoff, Amit Lachman, Asaf Moran, Dan Vilenchik, Shlomit Fedida-Metula
Summary: The demand for poultry meat and eggs is growing consistently, and there is a pressing need for improved protocols to determine chicken sex, especially in the layer industry. Extensive efforts are being made to develop in-ovo sexing detection methods to avoid the culling of male chicks. In this study, a fast, cost-effective, and accurate method for genotyping sex chromosomes in chickens using small volumes of blood was developed and validated.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Biophysics
Qi Xu, Jinze Li, Zhiqi Zhang, Qi Yang, Wei Zhang, Jia Yao, Yaxin Zhang, Yueye Zhang, Zhen Guo, Chao Li, Shuli Li, Changsong Zhang, Chuanxin Wang, Lutao Du, Chuanyu Li, Lianqun Zhou
Summary: Real-time digital polymerase chain reaction (qdPCR) integrates absolute quantification with process information, improving precision in molecular diagnostics. We propose a novel approach to determine optimal reaction conditions for qdPCR using real-time fluorescence monitoring in microwells. By leveraging an ultrathin laminated chip (UTL chip), we achieve faster amplification and higher accuracy, showing promise for molecular diagnostics.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Multidisciplinary Sciences
Walid Hossain, Mohabbat Hossain, Khalid Arafath, Subarna Sayed Ety, Mahade Hasan Shetu, Mazbahul Kabir, Farjana Akther Noor, Kalissar Mannoor
Summary: The study aimed to reduce the run time of multiplex RT-PCR for detecting SARS CoV-2 N1 and N2 sequences and human RNase P (RP) sequence. By optimizing the Fast PCR protocol and comparing it with conventional PCR, the researchers found that Fast PCR significantly shortened the run time while yielding comparable results to conventional PCR.
Article
Agriculture, Multidisciplinary
A-Tai Truong, Seonmi Kim, Byoungsu Yoon
Summary: A novel molecular method using UR-qPCR was developed for rapid amplification and quantification of maize residual DNA in honey, showing the ability to identify a minimum of 10% adulteration of corn syrup in honey. Detection of both pollen and residual DNA of maize in honey is crucial in identifying the source of maize residual DNA present in honey. This rapid PCR assay is a useful tool for specific identification of the corn syrup used for honey adulteration.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Analytical
Yuwei Qiu, Kunlun Jiang, Jingrui Wu, Yung-Kang Peng, Jeong-Wook Oh, Jung- Hoon Lee
Summary: A plasmonic photothermal quantitative immuno-PCR (PPT-qiPCR) assay is developed for biomarker detection using multifunctional plasmonic immunomagnetic nanoparticles (PIMNs). This assay has real-time and end-point dual readout and is simple, time-saving, and inexpensive compared to conventional iPCR platforms. It can quickly discriminate target proteins and improve assay sensitivity with in-situ end-point fluorescence or colorimetric detection using only the human eye.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Multidisciplinary Sciences
Anna Poma, Patrizia Cesare, Antonella Bonfigli, Anna Rita Volpe, Sabrina Colafarina, Giulia Vecchiotti, Alfonso Forgione, Osvaldo Zarivi
Summary: Molecular biology techniques are increasingly used in identifying the sex of skeletal remains, especially when traditional anthropometric analyzes are not successful. In this study, a qPCR-duplex method was used to determine the sex of both ancient and modern DNA samples. The validity of the method was verified on modern DNA, showing 100% accuracy in gender identification. This molecular technique is sensitive and precise, even on degraded DNA.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Wenhuan Kuang, Congcong Yuan, Jie Zhang
Summary: The authors propose a deep learning method to determine the source focal mechanism of earthquakes in real-time. They trained their network with approximately 800k synthetic samples and managed to successfully estimate the focal mechanism of four 2019 Ridgecrest earthquakes with magnitudes larger than Mw 5.4.
NATURE COMMUNICATIONS
(2021)
