Article
Engineering, Chemical
Xiangzhi Zhang, Xiawei Xu, Yong Ren, Yuying Yan, Aiguo Wu
Summary: An Y-Y shaped microfluidic device was designed to separate NSCLC cells using a dielectrophoresis approach, with a separation efficiency of around 99% achieved under optimal operating conditions. This study presents a potentially efficient, facile, and low-cost route for circulating tumor cell separation, which was optimized through numerical simulations.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Reza Derakhshan, Amirhosein Ghasemi, Reza Moradi, Abas Ramiar
Summary: This paper presents a comprehensive numerical investigation on particle/cell separation in circular microchannels using dielectrophoresis (DEP). The results show that the direction of particle deflection can be controlled by varying the geometrical parameters. By applying DEP, efficient separation of particles and cells based on their size and physical properties is achieved.
ADVANCED POWDER TECHNOLOGY
(2023)
Review
Engineering, Chemical
Ioannis H. Karampelas, Jenifer Gomez-Pastora
Summary: The demand for precise separation of particles, cells, and other biological matter has increased in recent years, leading to increased scientific interest in the topic. This article establishes the theoretical basis for fluid flow and particle separation and summarizes computational work done in the field of particle and cell separation, with a focus on magnetic, dielectric, and acoustic methods.
Article
Biochemical Research Methods
Mohammad Mehdi Keumarsi, Pouria Feyzi Oskouei, Rasool Dezhkam, Amir Shamloo, Farzad Vatandoust, Hoseyn A. Amiri
Summary: An innovative integrated double stair-shaped microchannel is designed for blood separation and chemical lysis, with the ability to control lysis intensity by adjusting the concentration of lysis reagent. Insulator-based dielectrophoresis (iDEP) is utilized for maximum separation efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Engineering, Chemical
Haotian Cha, Hoseyn A. Amiri, Sima Moshafi, Ali Karimi, Ali Nikkhah, Xiangxun Chen, Hang T. Ta, Nam-Trung Nguyen, Jun Zhang
Summary: Inertial microfluidics is a technique that uses the finite inertia of fluid at high flow speed to manipulate and separate microparticles. Embedding periodic micro-obstacles into curvilinear channels has been found to be an effective strategy to improve inertial focusing and separation. This study systematically investigated the influence of micro-obstacles on inertial focusing and developed a high-resolution microfluidic device for particle and cell separation. The results showed that concave obstacles were more effective in tuning particle inertial focusing and separation compared to convex obstacles, and the square concave obstacle channel offered the highest separation resolution. The developed microfluidic device showed high-efficiency separation of polystyrene beads and U87MG cancer cells from blood.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Biochemical Research Methods
Joseph Bentor, Amirreza Malekanfard, Mahmud Kamal Raihan, Sen Wu, Xinxiang Pan, Yongxin Song, Xiangchun Xuan
Summary: Experimental study shows that rheological properties of fluids significantly impact the focusing and trapping effects in iDEP microdevices. Strongly viscoelastic polyacrylamide solution demonstrates the best performance, while xanthan gum solution is not suitable for iDEP focusing.
Article
Nanoscience & Nanotechnology
Ngoc-Viet Nguyen, Tu Le Manh, Tang Son Nguyen, Viet Thong Le, Nguyen Van Hieu
Summary: DEP is a cost-effective technique for manipulating biological particles in microfluidics. This study presents an advanced solution strategy for focusing and separating cancerous cells, with theoretical calculations and a simulation model determining optimal parameters. The proposed method shows potential as a simple, low-cost approach to enhance cancer detection at early stages.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2021)
Article
Mechanics
Mohammad Charjouei Moghadam, Armin Eilaghi, Pouya Rezai
Summary: This study used numerical simulation to investigate elasto-inertial focusing of microparticles in a straight microchannel, proposing a new threshold for particle dispersion prediction. An empirical non-dimensional correlation was developed to predict elasto-inertial particle dispersion in straight square cross-sectional microchannels, reducing the predicted dispersion variation from about 15% to less than about 5%.
Article
Biochemical Research Methods
Reza Derakhshan, Ali Bozorgzadeh, Abas Ramiar
Summary: In recent years, microfluidic-based particle/cell manipulation techniques, especially dielectrophoresis (DEP), have brought significant advances in various scientific fields. This study presents the design and investigation of a sheathless 3D microchannel with a wall-mounted trapezoidal obstacle for continuous-flow separation of polystyrene particles using DEP. A simulation code is developed and validated with experimental tests. The numerical investigation reveals that obstacle height, applied voltage, electrode pairs angle, and flow rate significantly affect efficient particle separation. The proposed design shows promise for integration with lab-on-a-chip and clinical diagnosis applications due to its advantages.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Acoustics
Lizhu Liu, Jian Zhou, Kaitao Tan, Hui Zhang, Xin Yang, Huigao Duan, YongQing Fu
Summary: This study develops a simplified but effective 3D SSAW microfluidic model to investigate the separation and manipulation of particles. The model considers propagation attenuation of surface waves to increase modeling accuracy and simplifies the modeling of piezoelectric substrates and microchannel walls to reduce computational complexity. Simulation results are compared with experimental data, demonstrating the reliability of the model.
Review
Chemistry, Analytical
Malihe Farasat, Ehsan Aalaei, Saeed Kheirati Ronizi, Atin Bakhshi, Shaghayegh Mirhosseini, Jun Zhang, Nam-Trung Nguyen, Navid Kashaninejad
Summary: Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics has enabled the miniaturization of analytical devices by controlling the motion of fluid in micrometer-sized channels and chambers. Among different microfluidic methods, dielectrophoresis (DEP) offers advantages for manipulating neutral bioparticles using unique dielectric characteristics. This review focuses on signal-based DEP methods that utilize signal parameters such as frequency, amplitude, phase, and shape for cell/particle separation and manipulation.
Article
Engineering, Aerospace
Robert E. Weber, Janusz J. Petkowski, Monika U. Weber
Summary: Based on evidence of chemical disequilibria and other anomalous observations, it is motivated that there may be life within the Venusian atmosphere. The Fluid-Screen technology, which utilizes dielectrophoretic microbial particle capture, concentration, and separation, is capable of capturing and separating various biological particles. This article discusses the potential implementation of Fluid-Screen in the context of the Venus Life Finder missions and highlights its unique scientific output.
Review
Chemistry, Multidisciplinary
Yalin Li, Yan Wang, Keming Wan, Mingxue Wu, Lei Guo, Xiaomin Liu, Gang Wei
Summary: This review summarizes the recent progress of dielectrophoresis (DEP) technology in biomedical applications, including the design and functional tailoring of DEP systems, as well as representative biomedical application examples. The article aims to provide new ideas for designing novel high-throughput DEP micro/nanoplatforms.
Article
Physics, Applied
D. Ince, H. Turhan, S. Cadirci, L. Trabzon
Summary: Passive cell separation methods have gained significant attention due to their ease of fabrication, precise manipulation, cost-effectiveness, sensitivity, and simplicity. This study investigated the influence of channel curvature angle on particle focusing using both experimental and numerical methods. The results showed that an increase in the channel curvature angle is a determining factor for particle focusing. The study also emphasized the importance of selecting appropriate particle size and concentration to achieve a single focus line.
JOURNAL OF APPLIED PHYSICS
(2022)
Article
Chemistry, Analytical
Arash Dalili, Hossein Montazerian, Kabilan Sakthivel, Nishat Tasnim, Mina Hoorfar
Summary: This study investigated the factors affecting the displacement of particles in electrophoresis-based microseparators, finding that volumetric throughput has the greatest impact on particle position, with channel length being the most effective factor. The study also successfully demonstrated size-selective sorting or focusing of particles, providing valuable insights into designing microfluidic platforms for selectively separating different cell types in biofluids.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Optics
Peyman Malekzadeh, Gholam-Mohammad Parsanasab, Hamed Nikbakht, Ezeddin Mohajerani, Majid Taghavi, Hamid Latifi
Summary: A novel method of increasing the extinction ratio of a plasmonic waveguide polarizer was proposed in this paper by coating aluminum in a multi-strip pattern. The simulation and experimental results showed that the extinction ratio of the multi-strip polarizer outperformed single-strip polarizers due to coupling loss and scattering effects. This polarizer demonstrated high extinction ratios at wavelengths of 1550 nm and 980 nm.
OPTICS COMMUNICATIONS
(2021)
Article
Engineering, Electrical & Electronic
Fatemeh Hamedi, Omid Reza Ranjbar-Naeini, Azam Layeghi, Amir Heidariazar, Mohammad Ismail Zibaii, Hamid Latifi
Summary: This paper presents a fiber-optic Fabry-Perot microcavity sensor based on splicing a catastrophic fused fiber (CFF) to a single-mode fiber (SMF) for simultaneous measurement of refractive index (RI) and temperature. By analyzing spatial frequency and wavelength shift, RI and temperature were effectively measured simultaneously.
OPTICAL FIBER TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Amir Asadollahi, Hamid Latifi, Shahriar Zeynali, Manojit Pramanik, Hamed Qazvini
Summary: This study investigates the accuracy of peak-power-compensation (PPC) in fiber-guided and free-space AR-PAM systems. The results show that tightly focused light confocal with the acoustic focus in a transparent medium can enhance the homogeneity of the illuminated sample image, while free-space propagation of laser pulses leads to more accurate PPC.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Behavioral Sciences
Leila Mohammadzadeh, Amir Mohammad Alizadeh, Mohammad Sadegh Feiz, Shole Jamali, Mohaddeseh Abedi, Hamid Latifi, Abbas Haghparast
Summary: This study found that acute administration of morphine increased neural activity and connectivity in the prefrontal cortex, while chronic administration of morphine decreased them. These effects were further enhanced during withdrawal syndrome in morphine-dependent rats.
BEHAVIOURAL BRAIN RESEARCH
(2022)
Article
Optics
Majid Taghavi, Abolfazl Abedi, Gholam-Mohammad Parsanasab, Mojtaba Rahimi, Mohammad Noori, Hamzeh Nourolahi, Hamid Latifi
Summary: In this paper, a closed-loop micro-opto-electro-mechanical system (MOEMS) accelerometer based on the Fabry-Pdrot interferometer is presented. By using a closed-loop detection method, it is possible to increase the measurement range without losing the resolution. The results show that the accelerometer has a linear response in the range of +/- 5 g.
Article
Medicine, Research & Experimental
Mahban Rahimifard, Zeinab Bagheri, Mahmoudreza Hadjighassem, Razieh Pourahmad Jaktaji, Ebrahim Behroodi, Hamed Haghi-Aminjan, Mahsa Azami Movahed, Hamid Latifi, Saereh Hosseindoost, Afshin Zarghi, Jalal Pourahmad
Summary: This study demonstrates that the new pyrazino[1,2-a]benzimidazole derivatives L1 and L2 can reduce the invasion of GBM cells by controlling the expression of genes related to migration and proliferation. Additionally, the design of a microfluidic platform and related cell culture protocols facilitate the broad screening of 3D multicellular tumor spheroids derived from GBM tumor biopsies and provide effective support for drug development for brain gliomas.
Article
Biochemical Research Methods
Behnam B. K. Shariati, Seyyede Sarvenaz Khatami, Mohammad Ali Ansari, Fazel Jahangiri, Hamid Latifi, Valery V. Tuchin
Summary: This paper proposes a new strategy of optical clearing called time-dependent or temporal tissue optical clearing (TTOC) to enhance the optical imaging quality. By altering the pulse width, the absorption and scattering of light in tissue can be regulated in the TTOC technique. Experimental investigations and computational simulations were conducted to study the dependence of optical properties on pulse width in gelatin-based phantom and modeled human skin tissue. The results provide insights into the penetration depth and reflectance of light in tissue for different pulse widths.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biotechnology & Applied Microbiology
Shabnam Shahrivari, Neda Aminoroaya, Roya Ghods, Hamid Latifi, Seyedeh Atiyeh Afjei, Neda Saraygord-Afshari, Zeinab Bagheri
Summary: Evaluating the effectiveness of drugs on an individual basis before prescribing them is crucial in determining the right treatment plan. Microfluidic devices have emerged as a promising platform for mimicking natural microenvironments, allowing for more accurate drug testing. This study introduces a truncated microfluidic gradient maker that creates a linear concentration gradient of trastuzumab and investigates its effects on SK-BR-3 spheroid cells. The results show that trastuzumab has dose-dependent effects in both 2D and spheroid cell cultures, with lower IC50 values observed in the 3D spheroid models. Additionally, the microchip method requires fewer samples and reagents, reducing the risk of fluctuations and contamination.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Optics
Hamed Qazvini, Hamid Latifi, Amir Asadollahi
Summary: One of the main challenges in scanning microscopy is to increase the scanning speed without compromising the spatial resolution of the image. This paper presents a solution using Lissajous scanning and phase modulation techniques to achieve higher imaging speeds while maintaining resolution. Experimental results show that this approach can significantly improve both temporal and spatial resolution.
Article
Engineering, Electrical & Electronic
Mohammadreza Salehi Moghaddam, Hamid Latifi, Elham Abedini, Mohsen Ghazanfar, Ebrahim Behroodi, Mojde Salmani Sadeghi
Summary: The optical pH sensor based on smart hydrogel material was designed for microfluidic applications. The sensor made use of chitosan-TEOS interpenetrating network as a pH-sensitive component. By bonding the chitosan-TEOS-PDMS layer to a thin layer of PDMS, the sensor can be easily integrated into microfluidic chips. The thickness of the PDMS layer can be adjusted to control the sensitivity of the sensor. The sensor's response to pH changes was measured using the optical astigmatism method, and the experimental results showed its stability and sensitivity.
IEEE SENSORS JOURNAL
(2023)
Article
Engineering, Mechanical
Hosein Eghdami Estarki, Zahra Saeedian Tareie, Hamid Latifi
Summary: This paper presents simulation and experimental results of measuring pressure drop in a rectangular microfluidic channel using two Fabry-Perot (FP) flow sensors. The sensors have high sensitivity, no hysteresis, and a resolution of 33 nL/min, according to the experimental results.
FLOW MEASUREMENT AND INSTRUMENTATION
(2023)
Article
Chemistry, Medicinal
Afshin Zare, Alireza Afshar, Arezoo Khoradmehr, Neda Baghban, Gholamhossein Mohebbi, Alireza Barmak, Adel Daneshi, Afshar Bargahi, Iraj Nabipour, Sahar Almasi-Turk, Alireza Arandian, Mohammad Ismail Zibaii, Hamid Latifi, Amin Tamadon
Summary: Currently, there is a growing interest in using natural resources, such as oceans and marine environments, for the treatment of various types of cancers. This study focused on the anticancer properties of the venom from Cassiopea andromeda and Catostylus mosaicus jellyfish species against human pulmonary adenocarcinoma cells. The results showed that both venoms exhibited dose-dependent anti-tumoral effects and induced apoptosis in the cancer cells.
Article
Optics
A. Heidariazar, H. Latifi, M. Lotfollahi, M. Barzi
Summary: This article experimentally investigates the effect of seed power spectral density (PSD) on spectral broadening in a high-power narrow-linewidth fiber amplifier based on fiber Bragg grating (FBG) structure. The study demonstrates that increasing the PSD of the seed source can reduce the spectral bandwidth of the amplifier output. The experimental results provide valuable insights for power scaling in the spectral beam combining technique.
Article
Optics
A. Heidariazar, H. Latifi, M. Lotfollahi, M. Barzi, H. Asgharzadeh
Summary: This work presents experimental investigations on the variation of spectral broadening and optical efficiency in a kW-level narrow linewidth MOPA fiber laser under different pumping configurations. The results show that the backward-pumping scheme can reduce the spectral broadening and enhance the optical efficiency compared with the forward-pumping scheme.
Article
Nanoscience & Nanotechnology
Esra Ermis, Zeinab Bagheri, Ebrahim Behroodi, Hamid Latifi, Mahban Rahimifard, Elham Ajorlou
Summary: In this study, a one-pot, water-based synthesis method was presented for the production of red emissive biocompatible carbon dots (R-CDs) used for live bioimaging of U87 microglia tumor spheroids in a microwell-based microfluidic device. The optical and physical properties of the synthesized R-CDs were validated using various analytical methods. The prepared R-CDs exhibited low cytotoxicity and were successfully applied for live 3D cell imaging.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2022)
