期刊
ELECTROPHORESIS
卷 35, 期 8, 页码 1160-1164出版社
WILEY
DOI: 10.1002/elps.201300588
关键词
Bacterial aggregation; Capillary electrophoresis; Modified cell surface charge; Zeta potential
资金
- National Science Centre in Warsaw, Poland [N N204 369040]
- European Social Fund
- Polish National Budget
- Kujawsko-Pomorskie Region as part of the Krok w przyszlosc (Step into the Future) Sectoral Operational Program-Human Resources
Uncontrolled aggregation of bacterial cells is a significant disadvantage of electrophoretic separations. Various aspects of the electrophoretic behavior of different strains of Gram-positive Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus(1), and Micrococcus luteus bacteria and Gram-negative Escherichia coli bacteria were investigated in this study. Our findings indicate that bacteria can be rapidly analyzed by CZE with surface charge modification by calcium ions (Ca2+). Bound Ca2+ ions increase zeta potential to more than 2.0 mV and significantly reduce repulsive forces. Under the above conditions, bacterial cells create compact aggregates, and fewer high-intensity signals are observed in electropherograms. The above can be attributed to the bridging effect of Ca2+ between bacterial cells. CE was performed to analyze bacterial aggregates in an isotachophoretic mode. A single peak was observed in the electropherogram.
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